RAMP1 Antibody [N11L21]

Catalog No.: F2813

Application: Reactivity: Mouse, Rat, Human

Lane 1: Caco-2, Lane 2: Mouse brain, Lane 3: Rat brain

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:10000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
17 kDa

Datasheet & SDS

生物学的記述

Specificity
RAMP1 Antibody [N11L21] detects endogenous levels of total RAMP1 protein.

Clone
N11L21

Synonym(s)
Receptor activity-modifying protein 1; Calcitonin-receptor-like receptor activity-modifying protein 1; CRLR activity-modifying protein 1; RAMP1

Background
RAMP1 (Receptor Activity-Modifying Protein 1) is a single-transmembrane accessory protein belonging to the RAMP family (RAMP1-3) that partners with Class B GPCRs, particularly calcitonin receptor-like receptor (CLR), to form the functional CGRP receptor essential for CGRP signaling. RAMP1 consists of 148 amino acids with a large extracellular N-terminal domain adopting a three-helix bundle fold stabilized by three disulfide bonds, a single α-helical transmembrane domain, and a short intracellular C-terminal tail; key residues such as Y66, F93, H97, and F101 cluster on one face of the extracellular domain to mediate CLR binding near the plasma membrane. RAMP1 acts as a chaperone facilitating CLR maturation, terminal glycosylation, and trafficking from the endoplasmic reticulum/Golgi to the cell surface, while its extracellular domain forms a composite ligand-binding pocket with CLR's N-terminus, conferring CGRP specificity over related peptides like adrenomedullin. The CLR-RAMP1 heterodimer couples to Gαs, activating adenylyl cyclase to produce cAMP and downstream effects including vasodilation and neurogenic inflammation. RAMP1 dysregulation contributes to migraine pathophysiology via enhanced CGRP signaling and cancer progression through altered receptor trafficking.

Background

RAMP1 (Receptor Activity-Modifying Protein 1) is a single-transmembrane accessory protein belonging to the RAMP family (RAMP1-3) that partners with Class B GPCRs, particularly calcitonin receptor-like receptor (CLR), to form the functional CGRP receptor essential for CGRP signaling. RAMP1 consists of 148 amino acids with a large extracellular N-terminal domain adopting a three-helix bundle fold stabilized by three disulfide bonds, a single α-helical transmembrane domain, and a short intracellular C-terminal tail; key residues such as Y66, F93, H97, and F101 cluster on one face of the extracellular domain to mediate CLR binding near the plasma membrane. RAMP1 acts as a chaperone facilitating CLR maturation, terminal glycosylation, and trafficking from the endoplasmic reticulum/Golgi to the cell surface, while its extracellular domain forms a composite ligand-binding pocket with CLR's N-terminus, conferring CGRP specificity over related peptides like adrenomedullin. The CLR-RAMP1 heterodimer couples to Gαs, activating adenylyl cyclase to produce cAMP and downstream effects including vasodilation and neurogenic inflammation. RAMP1 dysregulation contributes to migraine pathophysiology via enhanced CGRP signaling and cancer progression through altered receptor trafficking.

References
[1] Barwell J, et al. Adv Exp Med Biol. 2012;744:13-24. [2] Hay DL, et al. Annu Rev Pharmacol Toxicol. 2016;56:469-87.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%