SESN1 Antibody [C3P2]

Catalog No.: F3463

Application: Reactivity: Mouse, Rat, Human

Lane 1: Ramos, Lane 2: K562, Lane 3: Hela, Lane 4: C2C12

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:10000 FCM1:100 - 1:500

Application
WB, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
57 kDa 65 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
SESN1 Antibody [C3P2] detects endogenous levels of total SESN1 protein.

Clone
C3P2

Synonym(s)
PA26; SEST1; SESN1; Sestrin-1; p53-regulated protein PA26

Background
SESN1 (Sestrin-1) is a highly conserved stress-inducible protein of the Sestrin family predominantly expressed in metabolically active tissues, including liver, skin, and heart. SESN1 contains conserved N-terminal and C-terminal domains responsible for its oxidoreductase function, crucial for regenerating peroxiredoxins and reducing intracellular peroxide levels, thereby protecting against oxidative stress. SESN1 acts as a critical regulator of cellular metabolism and homeostasis by activating AMP-activated protein kinase (AMPK) and inhibiting the mechanistic target of rapamycin complex 1 (mTORC1) pathway through interaction with TSC2, facilitating autophagy and suppressing protein synthesis under stress conditions. SESN1 also modulates reactive oxygen species (ROS) levels to prevent DNA damage and protein oxidation, linking it to the p53 tumor suppressor network and providing protection against cancer and neurodegenerative diseases. Through these mechanisms, SESN1 integrates cellular energetic status with adaptive stress responses, highlighting its role in tumor suppression, metabolic regulation, and neuroprotection. Its dysregulation or deficiency has been implicated in cancer progression and age-related pathologies.

Background

SESN1 (Sestrin-1) is a highly conserved stress-inducible protein of the Sestrin family predominantly expressed in metabolically active tissues, including liver, skin, and heart. SESN1 contains conserved N-terminal and C-terminal domains responsible for its oxidoreductase function, crucial for regenerating peroxiredoxins and reducing intracellular peroxide levels, thereby protecting against oxidative stress. SESN1 acts as a critical regulator of cellular metabolism and homeostasis by activating AMP-activated protein kinase (AMPK) and inhibiting the mechanistic target of rapamycin complex 1 (mTORC1) pathway through interaction with TSC2, facilitating autophagy and suppressing protein synthesis under stress conditions. SESN1 also modulates reactive oxygen species (ROS) levels to prevent DNA damage and protein oxidation, linking it to the p53 tumor suppressor network and providing protection against cancer and neurodegenerative diseases. Through these mechanisms, SESN1 integrates cellular energetic status with adaptive stress responses, highlighting its role in tumor suppression, metabolic regulation, and neuroprotection. Its dysregulation or deficiency has been implicated in cancer progression and age-related pathologies.

References
[1] Chen Y, et al. Cell Mol Biol Lett. 2022 Jan 3;27(1):2. [2] Rai N, et al. Ageing Res Rev. 2020 Dec;64:101186.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%