Somatostatin Receptor 1/SSTR1 Antibody [F9M21]

Catalog No.: F2483

Application: Reactivity: Human

Lane 1: Human fetal lung, Lane 2: Human small intestine

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:500 IF1:250 - 1:500

Application
WB, IF

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
43 kDa

Datasheet & SDS

生物学的記述

Specificity
Somatostatin Receptor 1/SSTR1 Antibody [F9M21] detects endogenous levels of total Somatostatin Receptor 1/SSTR1 protein.

Clone
F9M21

Synonym(s)
Somatostatin receptor type 1; SS-1-R; SS1-R; SS1R; SST1; SRIF-2; SSTR1

Background
Somatostatin Receptor 1 (SSTR1) is a G protein-coupled receptor (GPCR) subtype belonging to the somatostatin receptor family that mediates inhibitory effects of the peptide hormone somatostatin, regulating hormone secretion, cell proliferation, and neurotransmission in the brain, kidney, and lung. SSTR1 features the GPCR hallmark of seven transmembrane α-helices, an extracellular N-terminus, and intracellular C-terminus, with conserved motifs such as the DRY motif in the third intracellular loop critical for signal transduction. Key ligand-binding residues include Q6.55 and S7.34, which form part of the extracellular loops that create the ligand-binding pocket, showing higher affinity for somatostatin-14 (SRIF-14) over SRIF-28. The SSTR1 gene is intronless and located on chromosome 14q13. It couples to pertussis toxin-sensitive Giα proteins to inhibit adenylyl cyclase, reducing intracellular cAMP levels, modulate Ca2+ and K+ channels to hyperpolarize cells, and activate protein tyrosine phosphatases that mediate antiproliferative effects via MAPK pathway modulation. SSTR1 can form homo- and heterodimers (with SSTR5), which influence receptor signaling. Regulation of SSTR1 includes agonist-induced phosphorylation, rapid desensitization, and notably, unlike other SSTRs, limited internalization leading to potential upregulation on the cell surface, affecting therapeutic outcomes. Clinically, SSTR1 is highly expressed in neuroendocrine tumors, acromegaly, and various cancers (pituitary, colorectal, breast, gliomas), where it modulates hormone secretion, tumor growth, and neuronal signaling.

Background

Somatostatin Receptor 1 (SSTR1) is a G protein-coupled receptor (GPCR) subtype belonging to the somatostatin receptor family that mediates inhibitory effects of the peptide hormone somatostatin, regulating hormone secretion, cell proliferation, and neurotransmission in the brain, kidney, and lung. SSTR1 features the GPCR hallmark of seven transmembrane α-helices, an extracellular N-terminus, and intracellular C-terminus, with conserved motifs such as the DRY motif in the third intracellular loop critical for signal transduction. Key ligand-binding residues include Q6.55 and S7.34, which form part of the extracellular loops that create the ligand-binding pocket, showing higher affinity for somatostatin-14 (SRIF-14) over SRIF-28. The SSTR1 gene is intronless and located on chromosome 14q13. It couples to pertussis toxin-sensitive Giα proteins to inhibit adenylyl cyclase, reducing intracellular cAMP levels, modulate Ca2+ and K+ channels to hyperpolarize cells, and activate protein tyrosine phosphatases that mediate antiproliferative effects via MAPK pathway modulation. SSTR1 can form homo- and heterodimers (with SSTR5), which influence receptor signaling. Regulation of SSTR1 includes agonist-induced phosphorylation, rapid desensitization, and notably, unlike other SSTRs, limited internalization leading to potential upregulation on the cell surface, affecting therapeutic outcomes. Clinically, SSTR1 is highly expressed in neuroendocrine tumors, acromegaly, and various cancers (pituitary, colorectal, breast, gliomas), where it modulates hormone secretion, tumor growth, and neuronal signaling.

References
[1] Nagarajan SK, et al. ACS Omega. 2020 Aug 11;5(33):21145-21161. [2] Wang Y, et al. Proc Natl Acad Sci U S A. 2024 Oct 8;121(41):e2400298121.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%