SOX10 Antibody [E22E2]

Catalog No.: F4814

Application: Reactivity: Mouse, Rat, Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000-1:10000 IP1:30 IHC1:1000

Application
WB, IP, IHC

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
270 kDa 314 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
SOX10 Antibody [E22E2] detects endogenous levels of total SOX10 protein.

Clone
E22E2

Synonym(s)
Transcription factor SOX-10

Background
SOX10, a SOX E-group transcription factor, is a master regulator of neural crest cell fate, orchestrating their specification, survival, migration, and differentiation into melanocytes, Schwann cells, and oligodendrocytes via DNA binding and coactivator recruitment. It consists of a central HMG-box DNA-binding domain that bends DNA at specific motifs, an N-terminal dimerization domain, dual transactivation domains (acidic K2/TAM and potent C-terminal TA/TAC), and regulatory phosphorylation and acetylation sites that fine-tune its activity. SOX10 primarily acts by occupying enhancers and promoters as a monomer and synergizing with lineage-defining transcription factors, such as Pax3→Mitf for melanocytes, Krox20→Egr2 for Schwann cells, and Olig1/2→Myrf for oligodendrocytes, to trigger stage-specific gene expression cascades, enforced through self-regulatory and partner-dependent mechanisms. This ensures proper formation of neural crest-derived glial cells in both the peripheral and central nervous systems, as well as the development of the inner ear, enteric nervous system, and oligodendroglial maturation. Loss of SOX10 disrupts neural crest cell development, while mutations cause disorders like Waardenburg syndrome, PCWH, and increase melanoma risk; its overexpression in tumors promotes stemness and cancer progression, all modulated by intricate auto-regulation and partner interactions.

Background

SOX10, a SOX E-group transcription factor, is a master regulator of neural crest cell fate, orchestrating their specification, survival, migration, and differentiation into melanocytes, Schwann cells, and oligodendrocytes via DNA binding and coactivator recruitment. It consists of a central HMG-box DNA-binding domain that bends DNA at specific motifs, an N-terminal dimerization domain, dual transactivation domains (acidic K2/TAM and potent C-terminal TA/TAC), and regulatory phosphorylation and acetylation sites that fine-tune its activity. SOX10 primarily acts by occupying enhancers and promoters as a monomer and synergizing with lineage-defining transcription factors, such as Pax3→Mitf for melanocytes, Krox20→Egr2 for Schwann cells, and Olig1/2→Myrf for oligodendrocytes, to trigger stage-specific gene expression cascades, enforced through self-regulatory and partner-dependent mechanisms. This ensures proper formation of neural crest-derived glial cells in both the peripheral and central nervous systems, as well as the development of the inner ear, enteric nervous system, and oligodendroglial maturation. Loss of SOX10 disrupts neural crest cell development, while mutations cause disorders like Waardenburg syndrome, PCWH, and increase melanoma risk; its overexpression in tumors promotes stemness and cancer progression, all modulated by intricate auto-regulation and partner interactions.

References
[1] Pingault V, et al. J Med Genet. 2022 Feb;59(2):105-114. [2] Wahlbuhl M, et al. Nucleic Acids Res. 2012 Jan;40(1):88-101.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%