Syntaxin-4 Antibody [C14K1]

Catalog No.: F4585

Application: Reactivity: Human

Lane 1: A172, Lane 2: K562, Lane 3: HCT116, Lane 4: IMR-32

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50 IF1:50

Application
WB, IP, IF

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
35 kDa

Datasheet & SDS

生物学的記述

Specificity
Syntaxin-4 Antibody [C14K1] detects endogenous levels of total Syntaxin-4 protein.

Clone
C14K1

Synonym(s)
Syntaxin-4; Renal carcinoma antigen NY-REN-31; STX4; STX4A

Background
Syntaxin-4 (STX4) is a t-SNARE protein of the SNARE superfamily that plays a pivotal role in intracellular vesicle trafficking and regulated membrane fusion across various cell types. Syntaxin-4 has a long cytoplasmic N-terminal region with two coiled-coil domains, the Hab domain (involved in auto-inhibition) and the SNARE motif, a single C-terminal transmembrane domain anchoring it to the plasma membrane, and a short extracellular C-terminus. The Hab domain can fold back onto the SNARE motif in a “closed” conformation stabilized by Munc18c binding, with key N-peptide residues mediating this interaction. Syntaxin-4 forms trans-SNARE complexes with SNAP23/25 and VAMP2/7/8 to mediate membrane fusion, thereby regulating processes such as GLUT4 translocation in muscle and adipose tissue for insulin-stimulated glucose uptake, insulin granule exocytosis in β-cells, AMPA receptor trafficking in neurons, lytic granule release in cytotoxic T cells, and hepatitis C virus (HCV) particle secretion. Its activity cycles between closed (Munc18c-bound, inactive) and open (SNARE-competent) states, transitions triggered by Rab GTPases and phosphorylation events (e.g., by PKC/PKA at N-terminal sites), and integrates with PI3K/Akt and Ca²⁺ signaling pathways. Dysregulation of Syntaxin-4 is linked to diabetes (via impaired GLUT4 trafficking), neurodegeneration, and viral pathogenesis.

Background

Syntaxin-4 (STX4) is a t-SNARE protein of the SNARE superfamily that plays a pivotal role in intracellular vesicle trafficking and regulated membrane fusion across various cell types. Syntaxin-4 has a long cytoplasmic N-terminal region with two coiled-coil domains, the Hab domain (involved in auto-inhibition) and the SNARE motif, a single C-terminal transmembrane domain anchoring it to the plasma membrane, and a short extracellular C-terminus. The Hab domain can fold back onto the SNARE motif in a “closed” conformation stabilized by Munc18c binding, with key N-peptide residues mediating this interaction. Syntaxin-4 forms trans-SNARE complexes with SNAP23/25 and VAMP2/7/8 to mediate membrane fusion, thereby regulating processes such as GLUT4 translocation in muscle and adipose tissue for insulin-stimulated glucose uptake, insulin granule exocytosis in β-cells, AMPA receptor trafficking in neurons, lytic granule release in cytotoxic T cells, and hepatitis C virus (HCV) particle secretion. Its activity cycles between closed (Munc18c-bound, inactive) and open (SNARE-competent) states, transitions triggered by Rab GTPases and phosphorylation events (e.g., by PKC/PKA at N-terminal sites), and integrates with PI3K/Akt and Ca²⁺ signaling pathways. Dysregulation of Syntaxin-4 is linked to diabetes (via impaired GLUT4 trafficking), neurodegeneration, and viral pathogenesis.

References
[1] Kennedy MJ, et al. Cell. 2010 Apr 30;141(3):524-35. [2] Teng FY, et al. Genome Biol. 2001;2(11):REVIEWS3012.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%