TEAD1 Antibody [H6K12]

Catalog No.: F4878

Application: Reactivity: Mouse, Rat, Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100 IHC1:1000 CHIP1:2000

Application
WB, IP, IHC, ChIP

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
48 kDa 50,70, 52 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
TEAD1 Antibody [H6K12] detects endogenous levels of total TEAD1 protein.

Clone
H6K12

Synonym(s)
TCF13; TEF1; TEAD1; Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; Transcription factor 13; TEAD-1; TCF-13

Background
TEAD1 is a member of the TEA domain (TEAD) family of transcription factors (TEAD1-4), which serve as key downstream effectors of the evolutionarily conserved Hippo signaling pathway that controls organ size, cell proliferation, and tumor suppression by regulating YAP/TAZ activity. Its N-terminal TEA DNA-binding domain (TEAD) adopts a three-helix bundle homeodomain fold, with key structural features including an L1 loop essential for cooperative binding to tandem M-CAT DNA sites, unstructured regions (e.g., residues 1–11, 24–31), and a hydrophobic core of 12 residues (e.g., Ile-13, Leu-21); chemical shift perturbations identify helix H3, L2 loop, and the back surface as the primary DNA-binding interface. TEAD1 primarily drives gene expression for cell proliferation and anti-apoptosis upon nuclear translocation of unphosphorylated YAP, forming YAP/TEAD1 complexes that bind TEA/ATTS consensus sequences to activate pro-growth genes, with critical roles in cardiac, skeletal, and smooth muscle development. In the Hippo-off state, YAP escapes LATS1/2 phosphorylation and 14-3-3 sequestration to enable this activity, while Hippo-on signaling inactivates it; TEAD1’s tissue-specific expression underscores its developmental importance. Dysregulated TEAD1 activity contributes to oncogenesis, as seen in YAP-driven hepatocellular carcinoma where dominant-negative TEAD1 suppresses tumorigenesis.

Background

TEAD1 is a member of the TEA domain (TEAD) family of transcription factors (TEAD1-4), which serve as key downstream effectors of the evolutionarily conserved Hippo signaling pathway that controls organ size, cell proliferation, and tumor suppression by regulating YAP/TAZ activity. Its N-terminal TEA DNA-binding domain (TEAD) adopts a three-helix bundle homeodomain fold, with key structural features including an L1 loop essential for cooperative binding to tandem M-CAT DNA sites, unstructured regions (e.g., residues 1–11, 24–31), and a hydrophobic core of 12 residues (e.g., Ile-13, Leu-21); chemical shift perturbations identify helix H3, L2 loop, and the back surface as the primary DNA-binding interface. TEAD1 primarily drives gene expression for cell proliferation and anti-apoptosis upon nuclear translocation of unphosphorylated YAP, forming YAP/TEAD1 complexes that bind TEA/ATTS consensus sequences to activate pro-growth genes, with critical roles in cardiac, skeletal, and smooth muscle development. In the Hippo-off state, YAP escapes LATS1/2 phosphorylation and 14-3-3 sequestration to enable this activity, while Hippo-on signaling inactivates it; TEAD1’s tissue-specific expression underscores its developmental importance. Dysregulated TEAD1 activity contributes to oncogenesis, as seen in YAP-driven hepatocellular carcinoma where dominant-negative TEAD1 suppresses tumorigenesis.

References
[1] Lin KC, et al. Trends Biochem Sci. 2017 Nov;42(11):862-872. [2] Li F, et al. Nucleic Acids Res. 2022 Dec 9;50(22):12723-12738.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%