TIMP2 Antibody [L18F24]

Catalog No.: F4624

Application: Reactivity: Human, Mouse, Monkey

Lane 1: HT-1080, Lane 2: A172, Lane 3: COS-7

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
22 kDa

Datasheet & SDS

生物学的記述

Specificity
TIMP2 Antibody [L18F24] detects endogenous levels of total TIMP2 protein.

Clone
L18F24

Synonym(s)
Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2 (TIMP-2); TIMP2

Background
TIMP2 (Tissue Inhibitor of Metalloproteinases 2) is the most abundant member of the TIMP family (TIMP1–4), a conserved group of secreted glycoproteins that regulate extracellular matrix (ECM) remodeling. TIMP2 is composed of two disulfide-stabilized domains: an N-terminal inhibitory domain (127 amino acids, with an oligosaccharide/oligonucleotide-binding fold, five-stranded β-barrel, three disulfide bonds, and an extended AB loop for MMP specificity) and a C-terminal netrin-like domain (67 amino acids, with three additional disulfide bonds facilitating hemopexin domain binding). The N-terminal Cys1 chelates the catalytic zinc in MMPs via a Met-Tyr pair, and six total disulfide bridges maintain the protein’s wedge-shaped conformation. TIMP2 irreversibly inhibits matrix metalloproteinases (particularly MMP-2 and MMP-9) by occupying their active sites, but also uniquely promotes pro-MMP2 activation by binding MT1-MMP homodimers on cell surfaces to form a TIMP2–proMMP2–MT1-MMP ternary complex, which is cleaved to generate active MMP2. TIMP2 also interacts with α3β1 integrin via an RGD-like motif in the BC loop, activating SHP-1 phosphatase to suppress endothelial cell proliferation, migration, and angiogenesis, and modulating ECM signaling through interactions with VEGFR2 and other integrins. TIMP2 is essential for tissue homeostasis, wound healing, and vascular remodeling, while its dysregulation contributes to cancer metastasis (through MMP imbalance), fibrosis (via excessive ECM deposition), and tumor angiogenesis.

Background

TIMP2 (Tissue Inhibitor of Metalloproteinases 2) is the most abundant member of the TIMP family (TIMP1–4), a conserved group of secreted glycoproteins that regulate extracellular matrix (ECM) remodeling. TIMP2 is composed of two disulfide-stabilized domains: an N-terminal inhibitory domain (127 amino acids, with an oligosaccharide/oligonucleotide-binding fold, five-stranded β-barrel, three disulfide bonds, and an extended AB loop for MMP specificity) and a C-terminal netrin-like domain (67 amino acids, with three additional disulfide bonds facilitating hemopexin domain binding). The N-terminal Cys1 chelates the catalytic zinc in MMPs via a Met-Tyr pair, and six total disulfide bridges maintain the protein’s wedge-shaped conformation. TIMP2 irreversibly inhibits matrix metalloproteinases (particularly MMP-2 and MMP-9) by occupying their active sites, but also uniquely promotes pro-MMP2 activation by binding MT1-MMP homodimers on cell surfaces to form a TIMP2–proMMP2–MT1-MMP ternary complex, which is cleaved to generate active MMP2. TIMP2 also interacts with α3β1 integrin via an RGD-like motif in the BC loop, activating SHP-1 phosphatase to suppress endothelial cell proliferation, migration, and angiogenesis, and modulating ECM signaling through interactions with VEGFR2 and other integrins. TIMP2 is essential for tissue homeostasis, wound healing, and vascular remodeling, while its dysregulation contributes to cancer metastasis (through MMP imbalance), fibrosis (via excessive ECM deposition), and tumor angiogenesis.

References
[1] Costanzo L, et al. Pulm Med. 2022 Dec 31;2022:3632764. [2] Stetler-Stevenson WG. Am J Pathol. 2023 Oct;193(10):1336-1352.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%