TLR4 Antibody [L6M8]

Catalog No.: F4571

Application: Reactivity: Human, Mouse, Rat, Porcine, Bovine, Mammal

Lane 1: Raw 264.7, Lane 2: K562

サイズ	価格(税別)	在庫状況
20uL	JPY 17900	国内在庫なし(納期7~10日)
100uL	JPY 38900	国内在庫なし(納期7~10日)
100uL*2	JPY 58400	お問い合わせ

代表番号: 045-509-1970\|電子メール：sales@selleck.co.jp よく尋ねられる質問

使用情報

Dilution
WB1:600-1:2000 IHC1:400 IF1:10-1:500 CHIP1:10-1:500

Application
WB, IHC, IF, FCM, ChIP, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Porcine, Bovine, Mammal

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
95.7 kDa

Datasheet & SDS

生物学的記述

Specificity
TLR4 Antibody [L6M8] detects endogenous levels of total TLR4 protein.

Clone
L6M8

Synonym(s)
76B357.1, ARMD10, CD284 antigen, CD284, TLR4 facs, TLR4 flow cytometry, TLR4 human, TLR4 ihc, TLR4 western blot, TLR4, TLR-4, toll like receptor 4 protein, TOLL, toll-like receptor 4

Background
TLR4 (Toll‑like receptor 4) is a type I transmembrane pattern‑recognition receptor of the Toll/IL‑1 receptor (TIR) family that detects Gram‑negative bacterial lipopolysaccharide and selected endogenous damage‑associated ligands and initiates innate immune signaling programs that couple microbial sensing to inflammatory and antiviral gene expression. The receptor contains an N‑terminal extracellular leucine‑rich repeat ectodomain that forms a curved solenoid scaffold for ligand recognition with the accessory protein MD‑2, a single transmembrane helix, and a cytoplasmic TIR domain that nucleates adaptor recruitment and signal propagation; functional signaling requires assembly of an LPS–CD14–MD‑2–TLR4 complex and dimerization of two TLR4–MD‑2 units at the plasma membrane. TLR4 expression is prominent on myeloid cells such as macrophages and dendritic cells and is also present on endothelial and epithelial cells where it operates as a primary sensor of circulating endotoxin and of endogenous alarmins, driving rapid transcriptional responses that include TNF, IL‑6, IL‑1 family cytokines, chemokines, co‑stimulatory molecules, and interferon‑regulated genes. Ligand engagement first activates a MyD88‑dependent cascade at the plasma membrane through recruitment of the TIR adaptors TIRAP/MAL and MyD88 to the TLR4 TIR domain, followed by assembly of IRAK kinases and TRAF6, activation of TAK1, and stimulation of the IKK complex and MAPKs, which leads to IκB degradation and NF‑κB nuclear translocation together with AP‑1 activation and robust production of pro‑inflammatory mediators. Subsequent endocytosis of the TLR4 complex and signaling from early endosomes uses TRAM and TRIF as TIR adaptors to activate TBK1 and IKKε, phosphorylate IRF3, and induce type I interferon and interferon‑inducible genes, while also engaging a TRIF–RIP1–IKK axis that reinforces NF‑κB‑ and MAPK‑dependent responses, so that TLR4 couples surface and endosomal platforms to integrate pro‑inflammatory and antiviral outputs. These MyD88‑ and TRIF‑dependent branches are modulated by regulatory proteins such as SIGIRR, SOCS, and A20 and by scaffold proteins including GIV/Girdin, which dampen excessive macrophage activation and shape the magnitude and duration of cytokine release, linking TLR4 signal strength to protection from infection on one side and to tissue‑damaging inflammation on the other.

Background

TLR4 (Toll‑like receptor 4) is a type I transmembrane pattern‑recognition receptor of the Toll/IL‑1 receptor (TIR) family that detects Gram‑negative bacterial lipopolysaccharide and selected endogenous damage‑associated ligands and initiates innate immune signaling programs that couple microbial sensing to inflammatory and antiviral gene expression. The receptor contains an N‑terminal extracellular leucine‑rich repeat ectodomain that forms a curved solenoid scaffold for ligand recognition with the accessory protein MD‑2, a single transmembrane helix, and a cytoplasmic TIR domain that nucleates adaptor recruitment and signal propagation; functional signaling requires assembly of an LPS–CD14–MD‑2–TLR4 complex and dimerization of two TLR4–MD‑2 units at the plasma membrane. TLR4 expression is prominent on myeloid cells such as macrophages and dendritic cells and is also present on endothelial and epithelial cells where it operates as a primary sensor of circulating endotoxin and of endogenous alarmins, driving rapid transcriptional responses that include TNF, IL‑6, IL‑1 family cytokines, chemokines, co‑stimulatory molecules, and interferon‑regulated genes. Ligand engagement first activates a MyD88‑dependent cascade at the plasma membrane through recruitment of the TIR adaptors TIRAP/MAL and MyD88 to the TLR4 TIR domain, followed by assembly of IRAK kinases and TRAF6, activation of TAK1, and stimulation of the IKK complex and MAPKs, which leads to IκB degradation and NF‑κB nuclear translocation together with AP‑1 activation and robust production of pro‑inflammatory mediators. Subsequent endocytosis of the TLR4 complex and signaling from early endosomes uses TRAM and TRIF as TIR adaptors to activate TBK1 and IKKε, phosphorylate IRF3, and induce type I interferon and interferon‑inducible genes, while also engaging a TRIF–RIP1–IKK axis that reinforces NF‑κB‑ and MAPK‑dependent responses, so that TLR4 couples surface and endosomal platforms to integrate pro‑inflammatory and antiviral outputs. These MyD88‑ and TRIF‑dependent branches are modulated by regulatory proteins such as SIGIRR, SOCS, and A20 and by scaffold proteins including GIV/Girdin, which dampen excessive macrophage activation and shape the magnitude and duration of cytokine release, linking TLR4 signal strength to protection from infection on one side and to tissue‑damaging inflammation on the other.

References
[1]Kawasaki T, et al. Front Immunol. 2014 Sep 25;5:461. [2] Molteni M, et al. Mediators Inflamm. 2016;2016:6978936.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%