TMEM111 Antibody [B9P11]

Catalog No.: F5124

Application: Reactivity: Human, Mouse, Rat, Pig

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:2000-1:10000 IHC1:250-1:1000 IF1:200-1:800

Application
WB, IHC, IF

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Pig

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
30 kDa 30 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
TMEM111 Antibody [B9P11] detects endogenous levels of total TMEM111 protein.

Clone
B9P11

Synonym(s)
ER membrane protein complex subunit 3; Transmembrane protein 111; EMC3; TMEM111

Background
TMEM111, or Transmembrane Protein 111, is an integral endoplasmic reticulum membrane protein of about 26 kilodaltons and is part of the TMEM family of poorly characterized multi-spanning membrane proteins that help regulate protein quality control and cellular homeostasis in tissues such as the brain, liver, and kidney. TMEM111 is a small, hydrophobic protein with multiple predicted transmembrane domains that form an intramembrane scaffold, and it has cytoplasmic loops that interact with ER-associated degradation machinery and the Bag3 chaperone complex, though its specific motifs and residues are not well-defined due to limited structural data. Its main function is to coordinate the ubiquitin-proteasome and ER-associated degradation pathways by tethering misfolded proteins to degradation complexes, facilitating their ubiquitination through E2 conjugases, and preventing toxic protein aggregation during ER stress, thus maintaining proteostasis and cell viability. TMEM111 also supports the Bag3-HSP70-HSC70 chaperone network to selectively degrade aggregation-prone proteins, connects ER stress responses to autophagy, and ensures proper protein turnover through lysosomal and proteasomal routes. When TMEM111 is dysfunctional, ER-associated degradation becomes less efficient, leading to protein accumulation implicated in diseases such as Alzheimer's, due to amyloid processing defects, and liver disorders from impaired hepatocyte homeostasis.

Background

TMEM111, or Transmembrane Protein 111, is an integral endoplasmic reticulum membrane protein of about 26 kilodaltons and is part of the TMEM family of poorly characterized multi-spanning membrane proteins that help regulate protein quality control and cellular homeostasis in tissues such as the brain, liver, and kidney. TMEM111 is a small, hydrophobic protein with multiple predicted transmembrane domains that form an intramembrane scaffold, and it has cytoplasmic loops that interact with ER-associated degradation machinery and the Bag3 chaperone complex, though its specific motifs and residues are not well-defined due to limited structural data. Its main function is to coordinate the ubiquitin-proteasome and ER-associated degradation pathways by tethering misfolded proteins to degradation complexes, facilitating their ubiquitination through E2 conjugases, and preventing toxic protein aggregation during ER stress, thus maintaining proteostasis and cell viability. TMEM111 also supports the Bag3-HSP70-HSC70 chaperone network to selectively degrade aggregation-prone proteins, connects ER stress responses to autophagy, and ensures proper protein turnover through lysosomal and proteasomal routes. When TMEM111 is dysfunctional, ER-associated degradation becomes less efficient, leading to protein accumulation implicated in diseases such as Alzheimer's, due to amyloid processing defects, and liver disorders from impaired hepatocyte homeostasis.

References
[1] McWilliams TG. J Cell Biol. 2023 Apr 3;222(4):e202302118. [2] Gok MO, et al. J Cell Biol. 2023 Apr 3;222(4):e202204021.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%