VEGFB Antibody [E11E24]

Catalog No.: F2353

Application: Reactivity: Rat, Mouse

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Rat, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
22 kDa 19 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
VEGFB Antibody [E11E24] detects endogenous levels of total VEGFB protein.

Clone
E11E24

Synonym(s)
VRF; VEGFB; Vascular endothelial growth factor B; VEGF-B; VEGF-related factor

Background
Vascular Endothelial Growth Factor B (VEGFB) is a homodimeric cytokine belonging to the PDGF/VEGF family that promotes angiogenesis and regulates lipid metabolism in metabolically active tissues such as the heart, skeletal muscle, and brown adipose tissue. VEGFB exists as two isoforms, VEGFB167 (heparin-binding, matrix-associated) and VEGFB186 (secreted form), both featuring a conserved cystine-knot motif with six cysteine residues forming three disulfide bonds that stabilize the characteristic VEGF dimer structure essential for receptor binding. VEGFB binds with high affinity to VEGFR-1 (Flt-1) primarily through specific residues in its receptor-binding loops, activating signaling cascades that support endothelial cell survival and proliferation without strong mitogenic activity compared to VEGF-A. VEGFB enhances vascular permeability and stabilization while uniquely regulating fatty acid transport across endothelial barriers via induction of translocase proteins like CD36 and FABP4/5, thereby coordinating blood vessel formation with tissue energy demands. VEGFB signals through VEGFR-1 homodimers or VEGFR-1/VEGFR-2 heterodimers, activating PI3K/Akt and PLCγ pathways to promote vessel maturation rather than sprouting angiogenesis. Dysregulated VEGFB contributes to cardiovascular pathologies, including ischemic heart disease and atherosclerosis, acting as a therapeutic target for improving cardiac vascularization and metabolic homeostasis.

Background

Vascular Endothelial Growth Factor B (VEGFB) is a homodimeric cytokine belonging to the PDGF/VEGF family that promotes angiogenesis and regulates lipid metabolism in metabolically active tissues such as the heart, skeletal muscle, and brown adipose tissue. VEGFB exists as two isoforms, VEGFB167 (heparin-binding, matrix-associated) and VEGFB186 (secreted form), both featuring a conserved cystine-knot motif with six cysteine residues forming three disulfide bonds that stabilize the characteristic VEGF dimer structure essential for receptor binding. VEGFB binds with high affinity to VEGFR-1 (Flt-1) primarily through specific residues in its receptor-binding loops, activating signaling cascades that support endothelial cell survival and proliferation without strong mitogenic activity compared to VEGF-A. VEGFB enhances vascular permeability and stabilization while uniquely regulating fatty acid transport across endothelial barriers via induction of translocase proteins like CD36 and FABP4/5, thereby coordinating blood vessel formation with tissue energy demands. VEGFB signals through VEGFR-1 homodimers or VEGFR-1/VEGFR-2 heterodimers, activating PI3K/Akt and PLCγ pathways to promote vessel maturation rather than sprouting angiogenesis. Dysregulated VEGFB contributes to cardiovascular pathologies, including ischemic heart disease and atherosclerosis, acting as a therapeutic target for improving cardiac vascularization and metabolic homeostasis.

References
[1] Iyer S, et al. FEBS J. 2011 Nov;278(22):4304-22. [2] Iyer S, et al. J Mol Biol. 2006 May 26;359(1):76-85.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%