ZO1 tight junction protein Antibody [A24G22]

Catalog No.: F2530

Application: Reactivity: Rat, Human, Mouse

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
IHC1:500 IF1:100 FCM1:60

Application
IHC, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Rat, Human, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
ZO1 tight junction protein Antibody [A24G22] detects endogenous levels of total ZO1 tight junction protein.

Clone
A24G22

Synonym(s)
ZO1; TJP1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1

Background
ZO-1 (Zonula Occludens-1) is a peripheral membrane phosphoprotein belonging to the membrane-associated guanylate kinase (MAGUK) family and serves as a critical scaffolding molecule in tight junctions. ZO-1 contains three PDZ domains that facilitate protein-protein interactions, an SH3 domain that binds various signaling proteins, and a guanylate kinase (GuK) domain connected by flexible hinge regions; its unique C-terminal region directly binds to the actin cytoskeleton, anchoring the tight junction complex to maintain cellular integrity. ZO-1 organizes transmembrane tight junction proteins such as occludin and claudins, linking them to cytosolic partners like ZO-2 and ZO-3, thus playing an essential role in assembling and maintaining the tight junction barrier that controls paracellular permeability. ZO-1 also acts as a signaling hub regulating cell growth, migration, angiogenesis, and inflammation and helps maintain epithelial cell polarity by preserving the boundary between apical and basolateral membrane domains. ZO-1 is vital for tissue homeostasis in barriers like intestinal epithelium and the blood-brain barrier, with its downregulation associated with diseases such as inflammatory bowel disease (IBD) and cancer progression, where compromised tight junctions contribute to pathological permeability and tumorigenesis.

Background

ZO-1 (Zonula Occludens-1) is a peripheral membrane phosphoprotein belonging to the membrane-associated guanylate kinase (MAGUK) family and serves as a critical scaffolding molecule in tight junctions. ZO-1 contains three PDZ domains that facilitate protein-protein interactions, an SH3 domain that binds various signaling proteins, and a guanylate kinase (GuK) domain connected by flexible hinge regions; its unique C-terminal region directly binds to the actin cytoskeleton, anchoring the tight junction complex to maintain cellular integrity. ZO-1 organizes transmembrane tight junction proteins such as occludin and claudins, linking them to cytosolic partners like ZO-2 and ZO-3, thus playing an essential role in assembling and maintaining the tight junction barrier that controls paracellular permeability. ZO-1 also acts as a signaling hub regulating cell growth, migration, angiogenesis, and inflammation and helps maintain epithelial cell polarity by preserving the boundary between apical and basolateral membrane domains. ZO-1 is vital for tissue homeostasis in barriers like intestinal epithelium and the blood-brain barrier, with its downregulation associated with diseases such as inflammatory bowel disease (IBD) and cancer progression, where compromised tight junctions contribute to pathological permeability and tumorigenesis.

References
[1] McNeil E, et al. Mol Biol Cell. 2006 Apr;17(4):1922-32. [2] Van Itallie CM, et al. Mol Biol Cell. 2009 Sep;20(17):3930-40.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%