BM213

製品コード：P1215 純度：98%

BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.

Amino-acid Sequence

CAS No.

サイズ	価格(税別)	在庫状況
5mg	JPY 19100	国内在庫あり
25mg	JPY 53400	国内在庫あり
バルクサイズ	バルク特価あり

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製品安全説明書

現在のバッチを見る： P121501 Water] 100 mg/mL] false] ] ] false] ] ] false 純度： 98%

化学情報

保管	3 years-20°C powder	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
			１ケ月-20°Cin solvent

In vitro
Batch:

Water : 100 mg/mL

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

生物活性

製品説明	BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.

In vitro
In vitro	BM213 is a potent and stable peptide C5aR1 agonists that display no C5aR2 activity and over 1000-fold selectivity for C5aR1 over C3aR. BM213 induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is functionally similar to C5a in human macrophage cytokine release assays and in a murine in vivo neutrophil mobilization assay.
細胞実験	細胞株	HMDMs, SHSY-5Y cells
	濃度	0.1-100 μM
	反応時間	0-6 h, 24 h
	実験の流れ	HMDMs are stimulated with LPS (10 ng/mL) alone or in the presence of BM213. The supernatant content of cytokines after 24 h stimulation is quantiﬁed using ELISA. For MTT cell survival assay, diﬀerentiated SH-SY5Y cells are treated with varying concentrations of BM213 following a 2.5 h incubation.

In Vivo
In vivo	BM213 shows antitumor activity in a mouse model of mammary carcinoma.
動物実験	動物モデル	Mammary carcinoma model mice bearing with EMT-6 cells
	投与量	1mg/kg
	投与経路	IOCV

参考
https://pubmed.ncbi.nlm.nih.gov/34762432/

参考

https://pubmed.ncbi.nlm.nih.gov/34762432/

製品情報

Appearance	White Powder
Peptide Content (by %N)	≥80%
Single Impurity (by HPLC)	≤1.0%
Assay (By Anhydrous, Acetic Acid-free)	95.0~105.0%
MS	Consistent
Packing	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt)	3 years-20°C powder
Shipping	Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)