Nivolumab (anti-PD-1)

製品コードA2002 別名: BMS-936558, ONO-4538, MDX-1106, Opdivo

For research use only.
Not for use in humans.

Nivolumab is a genetically engineered, fully human immunoglobulin (Ig) G4 monoclonal antibody directed against the negative immunoregulatory human cell surface receptor programmed death-1 (PD-1,PCD-1) with immune checkpoint inhibitory and antineoplastic activities. MW : 143.597 KD.

Nivolumab (anti-PD-1)
サイズ 価格(税別)
5mg JPY 119300

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(28)

質量管理及び製品安全説明書

PD-1/PD-L1阻害剤の選択性比較

生物活性

製品説明 Nivolumab is a genetically engineered, fully human immunoglobulin (Ig) G4 monoclonal antibody directed against the negative immunoregulatory human cell surface receptor programmed death-1 (PD-1,PCD-1) with immune checkpoint inhibitory and antineoplastic activities. MW : 143.597 KD.
同型 Human IgG4
ターゲット
PD-1/PD-L1 interaction [2]
(Cell-free assay)
PD-1/PD-L2 interaction [2]
(Cell-free assay)
2.52 nM 2.59 nM
体外試験

The cell binding ratio of 125I-Nivolumab and 125I-Nivolumab-DTPA to PD-1 is 13.78 ± 2.90% and 10.22 ± 2.62%, respectively. Conjugating of DTPA does not affect targeting of Nivolumab to PD-1 in vitro.[1]

体内試験

In SPECT images, lesions with high 99mTc-DTPA-nivolumab uptake and relatively clear background are shown at 6 h. Thereafter, the suspected intestinal thickening in Gd-free T1WI is observed at 2 h after the addition of Gd-DTPA-nivolumab.[1]

お薦めの試験操作(参考用のみ)

細胞試験:
  • Splenocytes were isolated from the spleens obtained from humanized PD-1 over-expressing mice following standard protocol. 125I labeled Nivolumab and Nivolumab-DTPA, respectively. To perform co-culture, 125I-Nivolumab and 125I-Nivolumab-DTPA (0.37 MBq/µg) were added to the freshly isolated splenocytes (1×106 cells) grown in 6-well plates, respectively. After incubation for 12 h at 4°C, cells were collected after rinsing three times with PBS. 125I radioactivity was determined in a gamma counter to evaluate their binding efficiency to PD-1.

動物試験:
  • Animal Models: CRC mouse models (highly expressed human PD-1 antigen)
    Dosages: 11.1 MBq/10 μg
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883027/
     

製品説明

CAS No. 946414-94-4
Formulation PBS buffer, pH 7.2
Isotype Human IgG4
Source CHO cells
Storage Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

技術サポート

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Handling Instructions

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よくある質問(FAQ)

質問:1:
How to store the antibody?

回答:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

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