Acetyl-α-Tubulin (Lys40) Antibody [F22D16]

Catalog No.: F2686

Application: Reactivity: bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:15000-1:30000

Application
WB, IHC

Source
Mouse Monoclonal Antibody

Reactivity
bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
Acetyl-α-Tubulin (Lys40) Antibody [F22D16] detects endogenous levels of total α-Tubulin protein only when it is Acetylated at Lys40.

Clone
F22D16

Synonym(s)
Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; TUBA4A; TUBA1

Background
Acetyl-α-tubulin (Lys40) represents the lysine 40-acetylated form of α-tubulin, a core subunit of the tubulin heterodimer family that polymerizes into microtubules, essential cytoskeletal elements in eukaryotic cells. Acetylation targets the ε-amino group of the highly conserved Lys40 residue within a flexible 20-amino-acid luminal loop (residues 37-56) that protrudes into the microtubule interior, where it folds into an irregular turn packing against the acetyltransferase TAT active site funnel formed by conserved residues like Gln58, Arg158, Ile64, and Asp157 for substrate recognition and deprotonation. This internal modification, unique among microtubule PTMs, occurs post-polymerization via TAT accessing the lumen through lattice openings. Lys40 acetylation enhances microtubule flexibility and resilience to mechanical stress by altering protofilament rotational angles and lattice stability, promoting long-lived microtubules critical for dendritic arborization, axon growth, intracellular transport, cell motility, and neuronal branching. It is dynamically reversed by HDAC6 deacetylation, integrating into mechanosensitive pathways where stable acetylated MTs support ciliary/flagellar beating and polarity establishment. Dysregulation links to neurodegeneration, as reduced acetylation impairs dendritic stability and locomotion in models like Drosophila atat mutants. .

Background

Acetyl-α-tubulin (Lys40) represents the lysine 40-acetylated form of α-tubulin, a core subunit of the tubulin heterodimer family that polymerizes into microtubules, essential cytoskeletal elements in eukaryotic cells. Acetylation targets the ε-amino group of the highly conserved Lys40 residue within a flexible 20-amino-acid luminal loop (residues 37-56) that protrudes into the microtubule interior, where it folds into an irregular turn packing against the acetyltransferase TAT active site funnel formed by conserved residues like Gln58, Arg158, Ile64, and Asp157 for substrate recognition and deprotonation. This internal modification, unique among microtubule PTMs, occurs post-polymerization via TAT accessing the lumen through lattice openings. Lys40 acetylation enhances microtubule flexibility and resilience to mechanical stress by altering protofilament rotational angles and lattice stability, promoting long-lived microtubules critical for dendritic arborization, axon growth, intracellular transport, cell motility, and neuronal branching. It is dynamically reversed by HDAC6 deacetylation, integrating into mechanosensitive pathways where stable acetylated MTs support ciliary/flagellar beating and polarity establishment. Dysregulation links to neurodegeneration, as reduced acetylation impairs dendritic stability and locomotion in models like Drosophila atat mutants. .

References
[1] Niu X, et al. PLoS One. 2023 Feb 24;18(2):e0280573. [2] Szyk A, et al. Cell. 2014 Jun 5;157(6):1405-1415.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%