Acetyl-CoA Carboxylase 2 Antibody [E20M20]

Catalog No.: F3039

Application: Reactivity: Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
280 kDa

Datasheet & SDS

生物学的記述

Specificity
Acetyl-CoA Carboxylase 2 Antibody [E20M20] detects endogenous levels of total Acetyl-CoA Carboxylase 2 protein.

Clone
E20M20

Synonym(s)
Acetyl-CoA carboxylase 2; ACC-beta; ACACB; ACC2; ACCB

Background
Acetyl-CoA Carboxylase 2 (ACC2) is a biotin-dependent carboxylase enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, primarily regulating fatty acid oxidation in oxidative tissues like heart and skeletal muscle. ACC2 features a multi-domain architecture including a biotin carboxylase (BC) domain that carboxylates biotin using ATP and bicarbonate, a biotin carboxyl carrier protein (BCCP) domain that shuttles the carboxyl group, and a carboxyltransferase (CT) domain that transfers it to acetyl-CoA. A unique N-terminal hydrophobic sequence targets and anchors ACC2 to the outer mitochondrial membrane, positioning it near carnitine palmitoyltransferase 1 (CPT1). ACC2 operates as a dimer or filament in its active form, with activity modulated by phosphorylation. Mitochondrially localized malonyl-CoA produced by ACC2 allosterically inhibits CPT1, preventing long-chain fatty acid entry into mitochondria for beta-oxidation during energy-replete states. This mechanism maintains metabolic balance by suppressing fat breakdown when synthesis predominates, with ACC2 knockout enhancing oxidation and insulin sensitivity. ACC2 dysregulation contributes to metabolic disorders, including obesity, type 2 diabetes, and cardiovascular disease.

Background

Acetyl-CoA Carboxylase 2 (ACC2) is a biotin-dependent carboxylase enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, primarily regulating fatty acid oxidation in oxidative tissues like heart and skeletal muscle. ACC2 features a multi-domain architecture including a biotin carboxylase (BC) domain that carboxylates biotin using ATP and bicarbonate, a biotin carboxyl carrier protein (BCCP) domain that shuttles the carboxyl group, and a carboxyltransferase (CT) domain that transfers it to acetyl-CoA. A unique N-terminal hydrophobic sequence targets and anchors ACC2 to the outer mitochondrial membrane, positioning it near carnitine palmitoyltransferase 1 (CPT1). ACC2 operates as a dimer or filament in its active form, with activity modulated by phosphorylation. Mitochondrially localized malonyl-CoA produced by ACC2 allosterically inhibits CPT1, preventing long-chain fatty acid entry into mitochondria for beta-oxidation during energy-replete states. This mechanism maintains metabolic balance by suppressing fat breakdown when synthesis predominates, with ACC2 knockout enhancing oxidation and insulin sensitivity. ACC2 dysregulation contributes to metabolic disorders, including obesity, type 2 diabetes, and cardiovascular disease.

References
[1] Abu-Elheiga L, et al. Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1444-9. [2] Wang Y, et al. Front Oncol. 2022 Mar 11;12:836058.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%