α-adaptin A Antibody [D21J17]

Catalog No.: F1926

Application: Reactivity: Bovine, Hamster, Human, Mouse, Non-human primate, Rat

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:200 IF1:1000-1:2000 FCM1:2000

Application
WB, IP, IHC, IF, FCM

Source
Mouse Monoclonal Antibody

Reactivity
Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
~100 kDa

Datasheet & SDS

生物学的記述

Specificity
α-adaptin A Antibody [D21J17] detects endogenous levels of total α-adaptin A protein.

Clone
D21J17

Synonym(s)
100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; Adaptor protein complex AP-2 subunit alpha-1; ADTAA; AP2-ALPHA; AP2A1; CLAPA1

Background
α-Adaptin is a crucial subunit of the adaptor protein complex 2 (AP-2), which belongs to the family of adaptor protein complexes involved in intracellular vesicle transport. α-adaptin forms part of the heterotetrameric AP-2 complex, which consists of two large subunits (α and β2), a medium subunit (μ2), and a small subunit (σ2). The α-adaptin subunit contains a C-terminal appendage domain that functions as a scaffolding platform for endocytic accessory proteins. It also possesses regions that bind polyphosphoinositide-containing lipids, positioning AP-2 on the plasma membrane. AP-2 operates primarily in clathrin-dependent endocytosis by linking clathrin lattices to cargo proteins and membrane lipids and facilitating the formation of clathrin-coated vesicles (CCVs). The α and σ subunits contribute to recognizing specific endocytosis signal motifs, such as [ED]-X-X-X-L-[LI], which enables selective cargo capture. AP-2 also plays roles in recycling synaptic vesicle membranes, post-endocytic trafficking, and possibly ARF6-regulated non-clathrin pathways. Dysfunction or mutation in AP-2 components, such as α-adaptin, disrupts vesicle formation and endocytosis.

Background

α-Adaptin is a crucial subunit of the adaptor protein complex 2 (AP-2), which belongs to the family of adaptor protein complexes involved in intracellular vesicle transport. α-adaptin forms part of the heterotetrameric AP-2 complex, which consists of two large subunits (α and β2), a medium subunit (μ2), and a small subunit (σ2). The α-adaptin subunit contains a C-terminal appendage domain that functions as a scaffolding platform for endocytic accessory proteins. It also possesses regions that bind polyphosphoinositide-containing lipids, positioning AP-2 on the plasma membrane. AP-2 operates primarily in clathrin-dependent endocytosis by linking clathrin lattices to cargo proteins and membrane lipids and facilitating the formation of clathrin-coated vesicles (CCVs). The α and σ subunits contribute to recognizing specific endocytosis signal motifs, such as [ED]-X-X-X-L-[LI], which enables selective cargo capture. AP-2 also plays roles in recycling synaptic vesicle membranes, post-endocytic trafficking, and possibly ARF6-regulated non-clathrin pathways. Dysfunction or mutation in AP-2 components, such as α-adaptin, disrupts vesicle formation and endocytosis.

References
[1] Gulbranson DR, et al. Dev Cell. 2019 Aug 19;50(4):436-446.e5. [2] Chang MP, et al. EMBO J. 1993 May;12(5):2169-80.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%