ATF-6 Antibody [G8H4]

Catalog No.: F4203

Application: Reactivity: Human, Mouse

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
90-100 kDa

Datasheet & SDS

生物学的記述

Specificity
ATF-6 (D4Z8V) Rabbit mAb detects endogenous levels of total ATF-6 protein.

Clone
G8H4

Synonym(s)
Cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha (ATF6-alpha); ATF6

Background
ATF-6 (Activating Transcription Factor 6) is a type II transmembrane glycoprotein belonging to the bZIP transcription factor family, residing in the endoplasmic reticulum (ER) membrane as a key sensor of the unfolded protein response (UPR). ATF6 features an N-terminal cytoplasmic domain with a basic leucine zipper (bZIP) DNA-binding motif and transcriptional activation regions facing the cytosol, a single transmembrane helix, and a C-terminal ER luminal domain that associates with BiP/GRP78 under homeostatic conditions. Upon ER stress from unfolded protein accumulation, BiP dissociates from ATF6, enabling its trafficking to the Golgi apparatus for sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P), which liberates the active N-terminal cytoplasmic fragment. This cleaved fragment translocates to the nucleus where it binds ER stress response elements (ERSE) to directly activate transcription of UPR target genes, including GRP78/BiP, GRP94, protein disulfide isomerase (PDI), XBP1, and CHOP, enhancing ER chaperone capacity, protein folding, ER-associated degradation (ERAD), and ER biogenesis to restore proteostasis and promote cell survival. Dysregulation of ATF6 contributes to diabetes through impaired insulin signaling, neurodegeneration via protein aggregation, and cancer progression.

Background

ATF-6 (Activating Transcription Factor 6) is a type II transmembrane glycoprotein belonging to the bZIP transcription factor family, residing in the endoplasmic reticulum (ER) membrane as a key sensor of the unfolded protein response (UPR). ATF6 features an N-terminal cytoplasmic domain with a basic leucine zipper (bZIP) DNA-binding motif and transcriptional activation regions facing the cytosol, a single transmembrane helix, and a C-terminal ER luminal domain that associates with BiP/GRP78 under homeostatic conditions. Upon ER stress from unfolded protein accumulation, BiP dissociates from ATF6, enabling its trafficking to the Golgi apparatus for sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P), which liberates the active N-terminal cytoplasmic fragment. This cleaved fragment translocates to the nucleus where it binds ER stress response elements (ERSE) to directly activate transcription of UPR target genes, including GRP78/BiP, GRP94, protein disulfide isomerase (PDI), XBP1, and CHOP, enhancing ER chaperone capacity, protein folding, ER-associated degradation (ERAD), and ER biogenesis to restore proteostasis and promote cell survival. Dysregulation of ATF6 contributes to diabetes through impaired insulin signaling, neurodegeneration via protein aggregation, and cancer progression.

References
[1] Haze K, et al. Mol Biol Cell. 1999 Nov;10(11):3787-99. [2] Lee EJ, et al. J Clin Invest. 2025 Feb 3;135(3):e175562.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%