ATP1B1 Antibody [A23C23]

Catalog No.: F2309

Application: Reactivity: Human, Mouse, Sheep, Pig, Dog

Lane 1: Hela, Lane 2: MCF7, Lane 3: U251

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000-1:10000 IHC1:200

Application
WB, IP, IHC, IF

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Sheep, Pig, Dog

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
35 kDa ~42-55 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
ATP1B1 Antibody [A23C23] detects endogenous levels of total ATP1B1 protein.

Clone
A23C23

Synonym(s)
adenosinetriphosphatase; ATP1B1; ATPase beta subunit; ATPase, Na+/K+ transporting; MGC1798; Na+, K+-ATPase; OTTHUMP00000032537; OTTHUMP00000032538; Sodium Potassium ATPase; ATP1B; ATP1B1; Atp4b; Atpb; Atpb-1; NKbeta1

Background
ATP1B1, the β1 regulatory subunit of the Na+/K+-ATPase within the P-type ATPase β-subunit family, forms obligate heterotetramers with α1-4 catalytic subunits to establish transmembrane electrochemical gradients essential for excitable tissues and epithelial vectorial transport. Its single-pass transmembrane helix and large extracellular domain stabilize α-subunit maturation, plasma membrane trafficking, and voltage-sensitive conformational cycling between E1P (Na+-occluded) and E2P (K+-occluded) states, while allosterically modulating Na+ affinity and ouabain sensitivity through direct interactions with the α-subunit actuator domain. Circadian regulation via CLOCK:BMAL1 binding to promoter E-boxes drives rhythmic Atp1b1 expression antiphasic to blood pressure, where DEC1 repression lowers β1 levels to elevate vascular tone; conversely, DEC1 deficiency enhances pump density and reduces hypertension through sustained membrane hyperpolarization. In renal distal tubules, ATP1B1 coordinates paracellular Cl- permeability via modulation of claudin-2/4/7 expression, while cardiac β1 sustains diastolic relaxation through optimized sarcolemmal K+ influx during repolarization. Rare mutations disrupt α-β assembly, causing familial hemiplegic migraine, while heterozygous variants associate with PCOS through insulin resistance and granulosa cell dysfunction.

Background

ATP1B1, the β1 regulatory subunit of the Na+/K+-ATPase within the P-type ATPase β-subunit family, forms obligate heterotetramers with α1-4 catalytic subunits to establish transmembrane electrochemical gradients essential for excitable tissues and epithelial vectorial transport. Its single-pass transmembrane helix and large extracellular domain stabilize α-subunit maturation, plasma membrane trafficking, and voltage-sensitive conformational cycling between E1P (Na+-occluded) and E2P (K+-occluded) states, while allosterically modulating Na+ affinity and ouabain sensitivity through direct interactions with the α-subunit actuator domain. Circadian regulation via CLOCK:BMAL1 binding to promoter E-boxes drives rhythmic Atp1b1 expression antiphasic to blood pressure, where DEC1 repression lowers β1 levels to elevate vascular tone; conversely, DEC1 deficiency enhances pump density and reduces hypertension through sustained membrane hyperpolarization. In renal distal tubules, ATP1B1 coordinates paracellular Cl- permeability via modulation of claudin-2/4/7 expression, while cardiac β1 sustains diastolic relaxation through optimized sarcolemmal K+ influx during repolarization. Rare mutations disrupt α-β assembly, causing familial hemiplegic migraine, while heterozygous variants associate with PCOS through insulin resistance and granulosa cell dysfunction.

References
[1] Nakashima A, et al. Hypertension. 2018 Sep;72(3):746-754. [2] Urbanek M, et al. Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8573-8.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%