Cleaved PARP (Asp214) Antibody [C3D4]

Catalog No.: F4985

Application: Reactivity: Mouse, Rat

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:100 IHC1:100 IF1:800 FCM1:100

Application
WB, IP, IHC, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
89 kDa

Datasheet & SDS

生物学的記述

Specificity
Cleaved PARP (Asp214) Antibody [C3D4] detects endogenous levels of the large fragment (89 kDa) of PARP protein only when cleaved at Asp214.

Clone
C3D4

Synonym(s)
Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1); DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1 (ADPRT 1); Parp1; Adprp; Adprt; Adprt1

Background
Cleaved PARP (Asp214) is the caspase-3-generated cleavage product of poly(ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme central to DNA repair. PARP-1 consists of an N-terminal DNA-binding domain with zinc fingers (residues 1–214), a central automodification domain, and a C-terminal catalytic domain. During apoptosis, caspase-3 cleaves PARP-1 specifically between Asp214 and Gly215, resulting in a 24 kDa DNA-binding fragment and an 89 kDa catalytic fragment. This cleavage inactivates PARP-1’s DNA repair capacity by disrupting DNA binding and poly(ADP-ribosyl)ation activity, thereby preventing NAD+ depletion, limiting necrosis, and promoting orderly apoptotic progression. The presence of cleaved PARP is widely used as a hallmark of apoptosis, signaling caspase activation and cellular disassembly.

Background

Cleaved PARP (Asp214) is the caspase-3-generated cleavage product of poly(ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme central to DNA repair. PARP-1 consists of an N-terminal DNA-binding domain with zinc fingers (residues 1–214), a central automodification domain, and a C-terminal catalytic domain. During apoptosis, caspase-3 cleaves PARP-1 specifically between Asp214 and Gly215, resulting in a 24 kDa DNA-binding fragment and an 89 kDa catalytic fragment. This cleavage inactivates PARP-1’s DNA repair capacity by disrupting DNA binding and poly(ADP-ribosyl)ation activity, thereby preventing NAD+ depletion, limiting necrosis, and promoting orderly apoptotic progression. The presence of cleaved PARP is widely used as a hallmark of apoptosis, signaling caspase activation and cellular disassembly.

References
[1] Los M, et al. Mol Biol Cell. 2002 Mar;13(3):978-88. [2] Herceg Z, et al. Mol Cell Biol. 1999 Jul;19(7):5124-33.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%