Myc tag Antibody [A14H15]

Catalog No.: F4919

Application: Reactivity:

Lane 1: HEK-293T, Lane 2: HEK-293T (MYC-GFP transfected)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:500 - 1:1000 IF1:400 FCM1:2000

Application
WB, IP. IHC, IF, FCM

Source
Mouse Monoclonal Antibody

Reactivity

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
48 kDa,49 kDa 48 kDa, 13 kDa, 45 kDa, 57 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Myc tag Antibody [A14H15] detects recombinant proteins containing the Myc epitope tag.

Clone
A14H15

Synonym(s)
BHLHE39; MYC; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; Transcription factor p64; bHLHe39

Background
The Myc tag is a widely used small epitope tag consisting of an 11-amino-acid peptide sequence (MEQKLISEEDL) derived from the c-Myc protein, belonging to the family of epitope tags used for labeling and detecting recombinant proteins across various expression systems, including bacteria, yeast, insect, and mammalian cells. The Myc tag is compact and hydrophilic, which minimizes interference with the native structure and function of the fused protein. It is genetically fused to target proteins via recombinant DNA technology, allowing easy detection with highly specific monoclonal antibodies. It enables diverse applications such as immunoblotting (Western blotting) to detect protein expression and size, immunoprecipitation for isolating protein complexes, immunostaining for visualizing localization in cells, and flow cytometry for quantification. Its small size (<2 kDa) ensures minimal perturbation of the target protein. The Myc tag can also be combined with other tags, enhancing purification and interaction studies, thus simplifying protein characterization and accelerating research in cellular and molecular biology.

Background

The Myc tag is a widely used small epitope tag consisting of an 11-amino-acid peptide sequence (MEQKLISEEDL) derived from the c-Myc protein, belonging to the family of epitope tags used for labeling and detecting recombinant proteins across various expression systems, including bacteria, yeast, insect, and mammalian cells. The Myc tag is compact and hydrophilic, which minimizes interference with the native structure and function of the fused protein. It is genetically fused to target proteins via recombinant DNA technology, allowing easy detection with highly specific monoclonal antibodies. It enables diverse applications such as immunoblotting (Western blotting) to detect protein expression and size, immunoprecipitation for isolating protein complexes, immunostaining for visualizing localization in cells, and flow cytometry for quantification. Its small size (<2 kDa) ensures minimal perturbation of the target protein. The Myc tag can also be combined with other tags, enhancing purification and interaction studies, thus simplifying protein characterization and accelerating research in cellular and molecular biology.

References
[1] Scarfe J, et al. Plant J. 2025 Jun;122(6):e70272. [2] Schüchner S, et al. Sci Signal. 2020 Jan 28;13(616):eaax9730.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%