PAF1 Antibody [D19B13]

Catalog No.: F6889

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Hela, Lane 2: PANC-1, Lane 3: RAW264.7, Lane 4: H-4-II-E

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
80 kDa

Datasheet & SDS

生物学的記述

Specificity
PAF1 Antibody [D19B13] detects endogenous levels of total PAF1 protein.

Clone
D19B13

Synonym(s)
RNA polymerase II-associated factor 1 homolog; hPAF1; Pancreatic differentiation protein 2; PAF1; PD2

Background
Paf1 (RNA polymerase II–associated factor 1) is a core subunit of the conserved Paf1 complex (Paf1C), a multifunctional elongation‑associated module that physically associates with the elongating RNA polymerase II (RNAPII) machinery and functions as a platform coordinating transcription, chromatin modification, and RNA‑processing events. The Paf1 complex contains Paf1, Ctr9, Cdc73, Leo1, Rtf1, and, in higher eukaryotes, Ski8, and it travels with RNAPII across the body of actively transcribed genes, where it interacts with Spt4–Spt5, FACT, and other elongation factors to influence polymerase processivity and chromatin integrity. A key mechanistic function of the Paf1C is stimulation of histone H2B ubiquitination, which in turn promotes histone H3 methylation and modulates nucleosome dynamics around transcribed regions, thereby shaping the chromatin landscape that RNAPII must traverse. Paf1C further couples transcription elongation to mRNA 3′‑end processing by recruiting the cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) complexes via the Cdc73 subunit, ensuring co‑transcriptional assembly of the 3′‑end‑processing machinery at gene termini. In addition, the Ski8 subunit of the Paf1C links the complex to the TRAMP/Ski/Exosome RNA‑surveillance network, enabling co‑transcriptional quality control and degradation of aberrant or non‑productive transcripts.

Background

Paf1 (RNA polymerase II–associated factor 1) is a core subunit of the conserved Paf1 complex (Paf1C), a multifunctional elongation‑associated module that physically associates with the elongating RNA polymerase II (RNAPII) machinery and functions as a platform coordinating transcription, chromatin modification, and RNA‑processing events. The Paf1 complex contains Paf1, Ctr9, Cdc73, Leo1, Rtf1, and, in higher eukaryotes, Ski8, and it travels with RNAPII across the body of actively transcribed genes, where it interacts with Spt4–Spt5, FACT, and other elongation factors to influence polymerase processivity and chromatin integrity. A key mechanistic function of the Paf1C is stimulation of histone H2B ubiquitination, which in turn promotes histone H3 methylation and modulates nucleosome dynamics around transcribed regions, thereby shaping the chromatin landscape that RNAPII must traverse. Paf1C further couples transcription elongation to mRNA 3′‑end processing by recruiting the cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) complexes via the Cdc73 subunit, ensuring co‑transcriptional assembly of the 3′‑end‑processing machinery at gene termini. In addition, the Ski8 subunit of the Paf1C links the complex to the TRAMP/Ski/Exosome RNA‑surveillance network, enabling co‑transcriptional quality control and degradation of aberrant or non‑productive transcripts.

References
[1] Zhang Y, et al. Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2153-8. [2] Crisucci EM, et al. Genet Res Int. 2011;2011:707641.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%