Phospho-EGFR (Tyr992) Antibody [F20E14]

Catalog No.: F3647

Application: Reactivity: Human

Lane 1: A431, Lane 2: A431 (EGF, 100 ng/mL, 37℃, 30 min)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:2000 FCM1:500-1:2000

Application
WB, IP, FCM

Source
Mouse Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
134 kDa 170 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Phospho-EGFR (Tyr992) Antibody [F20E14] detects endogenous levels of total EGFR protein only when it is phosphorylated at Tyr992.

Clone
F20E14

Synonym(s)
ERBB; ERBB1; HER1; EGFR; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Background
Phospho-EGFR (Tyr992) is a phosphorylation site on the Epidermal Growth Factor Receptor (EGFR), a transmembrane tyrosine kinase of the HER/ErbB family that regulates cell growth and survival. Tyr992 resides in the intracellular domain near the plasma membrane and, upon phosphorylation, serves as a high-affinity docking site for the SH2 domain of phospholipase C-gamma (PLCγ), leading to PLCγ activation. Activated PLCγ hydrolyzes PIP2 into diacylglycerol (DAG) and inositol trisphosphate (IP3), which trigger protein kinase C (PKC) activation and intracellular calcium release, respectively, promoting cell proliferation and differentiation. This phosphorylation typically occurs after EGF-induced EGFR dimerization. In contrast to other phosphorylation sites like Tyr1045 (recruiting c-Cbl for receptor ubiquitination and degradation) and Tyr1068 (binding Grb2 to activate Ras/MAPK signaling), Tyr992-mediated PLCγ signaling represents a distinct pathway. EGFR phosphorylation thus orchestrates diverse downstream effects through site-specific adaptor protein interactions, regulating processes critical in normal physiology and cancer pathogenesis.

Background

Phospho-EGFR (Tyr992) is a phosphorylation site on the Epidermal Growth Factor Receptor (EGFR), a transmembrane tyrosine kinase of the HER/ErbB family that regulates cell growth and survival. Tyr992 resides in the intracellular domain near the plasma membrane and, upon phosphorylation, serves as a high-affinity docking site for the SH2 domain of phospholipase C-gamma (PLCγ), leading to PLCγ activation. Activated PLCγ hydrolyzes PIP2 into diacylglycerol (DAG) and inositol trisphosphate (IP3), which trigger protein kinase C (PKC) activation and intracellular calcium release, respectively, promoting cell proliferation and differentiation. This phosphorylation typically occurs after EGF-induced EGFR dimerization. In contrast to other phosphorylation sites like Tyr1045 (recruiting c-Cbl for receptor ubiquitination and degradation) and Tyr1068 (binding Grb2 to activate Ras/MAPK signaling), Tyr992-mediated PLCγ signaling represents a distinct pathway. EGFR phosphorylation thus orchestrates diverse downstream effects through site-specific adaptor protein interactions, regulating processes critical in normal physiology and cancer pathogenesis.

References
[1] Bishayee A, et al. Mol Biol Cell. 1999 Mar;10(3):525-36. [2] Rotin D, et al. EMBO J. 1992 Feb;11(2):559-67.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%