Phospho-MCM2 (Ser139) Antibody [D19G1]

Catalog No.: F7069

Application: Reactivity: Human, Mouse, Rat, Hamster, Monkey, Dog

Lane 1: Hela, Lane 2: Hela (λ phosphatase and CIP treated)

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50 IF1:100 FCM1:200

Application
WB, IP, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Hamster, Monkey, Dog

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
125 kDa

Datasheet & SDS

生物学的記述

Specificity
Phospho-MCM2 (Ser139) Antibody [D19G1] detects endogenous levels of total MCM2 protein only when it is phosphorylated at Ser139.

Clone
D19G1

Synonym(s)
DNA replication licensing factor MCM2; MCM2

Background
Phospho‑MCM2 (Ser139) marks a key regulatory site on the MCM2 subunit of the MCM2‑7 replicative helicase, which licenses origins and drives DNA‑fork unwinding during S phase. MCM2 is part of a heterohexameric ring that assembles with MCM3–7 at origins as part of the pre‑replication complex, and phosphorylation of MCM2 at Ser139 by the Cdc7/Dbf4 kinase in late G1 and early S phase coincides with the activation of replication forks, linking origin firing to cell‑cycle progression. In mammalian cells, Cdc7‑dependent phosphorylation of Ser139, along with neighboring Ser27 and Ser41, enhances the ATPase activity of the MCM2‑7 ring, promotes chromatin‑bound MCM2 phosphorylation, and is required for the orderly initiation of DNA synthesis, while non‑phosphorylatable MCM2 variants impair replication initiation and reduce helicase efficiency. The MCM2‑7 complex additionally participates in replication‑fork stability and checkpoint responses by interacting with checkpoint and homologous‑recombination regulators, and its dynamic phosphorylation ensures that each origin fires only once per cycle and that stalled forks can be protected and restarted.

Background

Phospho‑MCM2 (Ser139) marks a key regulatory site on the MCM2 subunit of the MCM2‑7 replicative helicase, which licenses origins and drives DNA‑fork unwinding during S phase. MCM2 is part of a heterohexameric ring that assembles with MCM3–7 at origins as part of the pre‑replication complex, and phosphorylation of MCM2 at Ser139 by the Cdc7/Dbf4 kinase in late G1 and early S phase coincides with the activation of replication forks, linking origin firing to cell‑cycle progression. In mammalian cells, Cdc7‑dependent phosphorylation of Ser139, along with neighboring Ser27 and Ser41, enhances the ATPase activity of the MCM2‑7 ring, promotes chromatin‑bound MCM2 phosphorylation, and is required for the orderly initiation of DNA synthesis, while non‑phosphorylatable MCM2 variants impair replication initiation and reduce helicase efficiency. The MCM2‑7 complex additionally participates in replication‑fork stability and checkpoint responses by interacting with checkpoint and homologous‑recombination regulators, and its dynamic phosphorylation ensures that each origin fires only once per cycle and that stalled forks can be protected and restarted.

References
[1] Bailis JM, et al. Mol Cell Biol. 2008 Mar;28(5):1724-38. [2] Tsuji T, et al. Mol Biol Cell. 2006 Oct;17(10):4459-72.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%