Phospho-STAT1 (Ser727) Antibody [F23C16]

Catalog No.: F2358

Application: Reactivity: Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:10000 FCM1:1000-1:100000

Application
WB, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
87 kDa

Datasheet & SDS

生物学的記述

Specificity
Phospho-STAT1 (Ser727) Antibody [F23C16] detects endogenous levels of total STAT1 protein only when it is Phosphorylated at Ser727.

Clone
F23C16

Synonym(s)
Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1

Background
Phospho-STAT1 (Ser727) is the serine-phosphorylated form of STAT1 at residue Ser727, a modification that plays a pivotal role in regulating STAT1’s transcriptional activity within interferon (IFN) signaling. STAT1, a latent transcription factor, contains several domains: an N-terminal oligomerization domain, a coiled-coil domain, a DNA-binding domain, an SH2 domain for dimerization via Tyr701 phosphorylation, and a C-terminal transactivation domain (TAD) harboring the Ser727 phosphorylation motif (PMSP), which is targeted by MAPKs such as p38. Phosphorylation at Ser727 occurs downstream of IFN-α/γ, cellular stress, or LPS, dependent on prior Tyr701 activation, and enhances STAT1’s interaction with coactivators like CBP/p300. This modification is essential for maximal transcriptional activation of IFN-responsive genes: it augments promoter recruitment and chromatin binding, supporting antiviral responses, macrophage activation, and inflammation. Aberrant Ser727 phosphorylation is linked to tumorigenesis and impaired IFN signaling by disrupting normal STAT1 activity.

Background

Phospho-STAT1 (Ser727) is the serine-phosphorylated form of STAT1 at residue Ser727, a modification that plays a pivotal role in regulating STAT1’s transcriptional activity within interferon (IFN) signaling. STAT1, a latent transcription factor, contains several domains: an N-terminal oligomerization domain, a coiled-coil domain, a DNA-binding domain, an SH2 domain for dimerization via Tyr701 phosphorylation, and a C-terminal transactivation domain (TAD) harboring the Ser727 phosphorylation motif (PMSP), which is targeted by MAPKs such as p38. Phosphorylation at Ser727 occurs downstream of IFN-α/γ, cellular stress, or LPS, dependent on prior Tyr701 activation, and enhances STAT1’s interaction with coactivators like CBP/p300. This modification is essential for maximal transcriptional activation of IFN-responsive genes: it augments promoter recruitment and chromatin binding, supporting antiviral responses, macrophage activation, and inflammation. Aberrant Ser727 phosphorylation is linked to tumorigenesis and impaired IFN signaling by disrupting normal STAT1 activity.

References
[1] Sadzak I, et al. Proc Natl Acad Sci U S A. 2008 Jul 1;105(26):8944-9. [2] Pilz A, et al. EMBO Rep. 2003 Apr;4(4):368-73.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%