Thymine Dimer Antibody [E15K19]

Catalog No.: F2701

Application: Reactivity: All species

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution

Application
WB, IP, IHC, IF, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
All species

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
Thymine Dimer Antibody [E15K19] detects endogenous levels of total Thymine Dimer protein.

Clone
E15K19

Synonym(s)
Cyclobutane pyrimidine dimer (CPD)

Background
Thymine dimer (cis-syn cyclobutane thymine dimer, CPD) is the most abundant UV-B-induced DNA photolesion formed exclusively between adjacent thymine bases on the same strand via [2+2] cycloaddition, classified as a mutagenic non-coding lesion within the cyclobutane pyrimidine dimer family. The cis-syn isomer features covalent C5–C6 bonds forming a rigid cyclobutane ring that saturates the 5,6-double bonds of both thymines, eliminating aromaticity, bending the DNA helix by ~30°, unwinding the TT step by ~40°, widening both grooves, pinching the phosphate backbone, weakening 5'-T:A pairing, and inducing anomalous tilt/twist angles localized primarily to the lesion site while preserving overall B-form stacking. Thymine dimers block replicative DNA/RNA polymerases, triggering nucleotide excision repair (NER) via XPC/Rad4 recognition of helical distortion or photolyase direct reversal; translesion synthesis by Pol η inserts AA opposite TT to minimize mutations, but unrepaired lesions cause C→T transitions at dipyrimidine sites characteristic of skin cancers, including melanoma, basal/squamous cell carcinoma. Negative supercoiling enhances dimer formation by facilitating base unstacking.

Background

Thymine dimer (cis-syn cyclobutane thymine dimer, CPD) is the most abundant UV-B-induced DNA photolesion formed exclusively between adjacent thymine bases on the same strand via [2+2] cycloaddition, classified as a mutagenic non-coding lesion within the cyclobutane pyrimidine dimer family. The cis-syn isomer features covalent C5–C6 bonds forming a rigid cyclobutane ring that saturates the 5,6-double bonds of both thymines, eliminating aromaticity, bending the DNA helix by ~30°, unwinding the TT step by ~40°, widening both grooves, pinching the phosphate backbone, weakening 5'-T:A pairing, and inducing anomalous tilt/twist angles localized primarily to the lesion site while preserving overall B-form stacking. Thymine dimers block replicative DNA/RNA polymerases, triggering nucleotide excision repair (NER) via XPC/Rad4 recognition of helical distortion or photolyase direct reversal; translesion synthesis by Pol η inserts AA opposite TT to minimize mutations, but unrepaired lesions cause C→T transitions at dipyrimidine sites characteristic of skin cancers, including melanoma, basal/squamous cell carcinoma. Negative supercoiling enhances dimer formation by facilitating base unstacking.

References
[1] Rumora AE, et al. Biochemistry. 2008 Dec 9;47(49):13026-35. [2] Park H, et al. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15965-70.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%