ULK1 Antibody [A14P9]

Catalog No.: F0312

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: U20S, Lane 2: RD, Lane 3: C2C12, Lane 4: Vero

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

文献中Selleckの製品使用例(4)

使用情報

Dilution
WB1:1000 IP1:100

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
150 kDa

Datasheet & SDS

生物学的記述

Specificity
ULK1 (D8H5) Rabbit mAb detects endogenous levels of total ULK1 protein.

Clone
A14P9

Synonym(s)
Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; hATG1); Unc-51-like kinase 1; ULK1; KIAA0722

Background
ULK1 (Unc-51 Like Autophagy Activating Kinase 1) is a serine/threonine protein kinase belonging to the UNC-51/Atg1 family, mammalian homolog of C. elegans unc-51 and yeast Atg1, essential for autophagy initiation and neuronal axon guidance. ULK1 (1058 amino acids, ~140 kDa) comprises an N-terminal kinase domain (residues 1-280) with conserved catalytic triad, central proline/serine-rich (PS) domain (281-500) mediating protein interactions, and C-terminal domain (501-1058) with Atg13/FIP200-binding motifs and regulatory phosphorylation sites (Ser317/Ser555/Ser777 by AMPK; Ser757 by mTOR). ULK1 forms a stable complex with Atg13, FIP200, and Atg101 that localizes to ER tubulovesicular regions/Atg9 vesicles upon nutrient deprivation; AMPK phosphorylation activates ULK1 kinase activity, inducing autophosphorylation and Atg13/Atg101/FIP200 phosphorylation to nucleate phagophore assembly, while mTORC1 phosphorylation inhibits complex formation. ULK1 forms clusters of over 30 molecules to initiate autophagosome biogenesis and also has non-autophagic functions, such as mediating growth cone endocytosis for NGF trafficking (via SynGAP/syntenin) and regulating mitophagy. Dysregulation of ULK1 is implicated in neurodegeneration through axon defects, cancer, where suppressed autophagy aids tumor survival, and metabolic disorders.

Background

ULK1 (Unc-51 Like Autophagy Activating Kinase 1) is a serine/threonine protein kinase belonging to the UNC-51/Atg1 family, mammalian homolog of C. elegans unc-51 and yeast Atg1, essential for autophagy initiation and neuronal axon guidance. ULK1 (1058 amino acids, ~140 kDa) comprises an N-terminal kinase domain (residues 1-280) with conserved catalytic triad, central proline/serine-rich (PS) domain (281-500) mediating protein interactions, and C-terminal domain (501-1058) with Atg13/FIP200-binding motifs and regulatory phosphorylation sites (Ser317/Ser555/Ser777 by AMPK; Ser757 by mTOR). ULK1 forms a stable complex with Atg13, FIP200, and Atg101 that localizes to ER tubulovesicular regions/Atg9 vesicles upon nutrient deprivation; AMPK phosphorylation activates ULK1 kinase activity, inducing autophosphorylation and Atg13/Atg101/FIP200 phosphorylation to nucleate phagophore assembly, while mTORC1 phosphorylation inhibits complex formation. ULK1 forms clusters of over 30 molecules to initiate autophagosome biogenesis and also has non-autophagic functions, such as mediating growth cone endocytosis for NGF trafficking (via SynGAP/syntenin) and regulating mitophagy. Dysregulation of ULK1 is implicated in neurodegeneration through axon defects, cancer, where suppressed autophagy aids tumor survival, and metabolic disorders.

References
[1] Lin MG, et al. Curr Opin Cell Biol. 2016 Apr;39:61-8. [2] Zachari M, et al. Essays Biochem. 2017 Dec 12;61(6):585-596.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%