uPA Antibody [F9E24]

Catalog No.: F2741

Application: Reactivity: Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:5000 IF1:50 - 1:100

Application
WB, IF

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
48 kDa 49 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
uPA Antibody [F9E24] detects endogenous levels of total uPA protein.

Clone
F9E24

Synonym(s)
Urokinase-type plasminogen activator; U-plasminogen activator; uPA; PLAU

Background
Urokinase-type plasminogen activator (uPA, PLAU) is a 411-residue secreted serine protease of the chymotrypsin family essential for pericellular proteolysis in the plasminogen activation system. Synthesized as a single-chain proenzyme (scuPA, 52 kDa) with an N-terminal growth factor domain (GFD) for uPAR binding, a kringle domain (KD) mediating fibrin and plasminogen interactions, a connecting peptide, and a catalytic serine protease domain (SPD) containing the His204-Asp256-Ser344 triad, uPA is activated by plasmin cleavage to a two-chain form (tc-uPA). By binding uPAR on cell surfaces, uPA localizes plasmin generation, leading to extracellular matrix (ECM) degradation, activation of MMPs and latent growth factors, and facilitating cell invasion and migration, all tightly regulated by PAI-1/2 inhibitors. uPA/uPAR complexes interact with integrins and vitronectin to activate ERK/MAPK, Rac1, and FAK pathways, supporting cell proliferation, survival, and chemotaxis. uPA is physiologically vital for wound healing, angiogenesis, and embryogenesis, while pathologically, it drives cancer metastasis (with high uPA levels indicating poor prognosis), atherosclerosis, and chronic inflammation via ECM remodeling.

Background

Urokinase-type plasminogen activator (uPA, PLAU) is a 411-residue secreted serine protease of the chymotrypsin family essential for pericellular proteolysis in the plasminogen activation system. Synthesized as a single-chain proenzyme (scuPA, 52 kDa) with an N-terminal growth factor domain (GFD) for uPAR binding, a kringle domain (KD) mediating fibrin and plasminogen interactions, a connecting peptide, and a catalytic serine protease domain (SPD) containing the His204-Asp256-Ser344 triad, uPA is activated by plasmin cleavage to a two-chain form (tc-uPA). By binding uPAR on cell surfaces, uPA localizes plasmin generation, leading to extracellular matrix (ECM) degradation, activation of MMPs and latent growth factors, and facilitating cell invasion and migration, all tightly regulated by PAI-1/2 inhibitors. uPA/uPAR complexes interact with integrins and vitronectin to activate ERK/MAPK, Rac1, and FAK pathways, supporting cell proliferation, survival, and chemotaxis. uPA is physiologically vital for wound healing, angiogenesis, and embryogenesis, while pathologically, it drives cancer metastasis (with high uPA levels indicating poor prognosis), atherosclerosis, and chronic inflammation via ECM remodeling.

References
[1] Vincenza Carriero M, et al. Front Biosci (Landmark Ed). 2009 Jan 1;14(10):3782-94. [2] Crippa MP. Int J Biochem Cell Biol. 2007;39(4):690-4.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%