Isoliquiritigenin

製品コード：S2404 純度：99.26%

Isoliquiritigenin, an anti-tumor flavonoid from the root of Glycyrrhiza glabra, inhibits aldose reductase with an IC50 of 320 nM.

CAS No. 961-29-5

サイズ	価格(税別)	在庫状況
10mg	JPY 25500	国内在庫あり
50mg	JPY 85500	国内在庫あり
1g	JPY 415500	国内在庫なし(納期7~10日)

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現在のバッチを見る：純度： 99.26%

99.26

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Aldose Reductase阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報
MCF7 cells	Cytotoxicity assay	25-150 μM	72 h	Cytotoxicity against human MCF7 cells at 25 to 150 uM after 72 hrs by MTT assay
U937 cells	Function assay	5 ug/ml	24 h	Antiinflammatory activity in human U937 cells assessed as inhibition of LPS-induced CCL5 secretion at 5 ug/ml after 24 hrs by ELISA relative to control
dog MDCK cells	Function assay	20 ug/mL	1 h	Antiviral activity against Influenza virus A/Puerto Rico/8/34 H1N1 infected in dog MDCK cells assessed as inhibition of viral replication at 20 ug/mL incubated at 1 hr post-infection measured after 24 hrs by hemagglutininating unit assay relative to control
U937 cells	Function assay	5 ug/ml		Inhibition of LPS-induced AP-1 activation in human U937 cells at 5 ug/ml after 1 hr relative to control
J774.1 cells	Function assay		24 h	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=29.3 μM
colon 26-L5 cells	Cytotoxicity assay		72 h	Cytotoxicity against mouse colon 26-L5 cells after 72 hrs by MTT assay, IC50=21.8 μM
human T47D cells	Function assay		96 h	Estrogenic activity in human T47D cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol after 96 hrs by alamar blue assay
MCF7 cells	Function assay		96 h	Estrogenic activity in human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol after 96 hrs by alamar blue assay
mouse NIH3T3 cells	Function assay		8 h	Inhibition of cobalt chloride-induced HIF-1 activation expressed in mouse NIH3T3 cells after 8 hrs by luciferase reporter gene assay, IC50=4.8 μM
OE21 cells	Cytotoxicity assay		72 h	Cytotoxicity against human OE21 cells after 72 hrs by MTT assay, IC50=48 μM
dog MDCK cells	Function assay		8 h	Inhibition of PKC/p38MAPK-mediated nuclear-cytoplasmic viral ribonucleoprotein export in dog MDCK cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 8 hrs post-infection by inmmunofluorescence
HUVEC	Cytotoxicity assay		48 h	Toxicity against HUVEC incubated for 48 hrs by MTT assay, IC50=41.17 μM
K562 cells	Cytotoxicity assay		48 h	Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay, IC50=29.27 μM
KB cells	Growth inhibition assay			Compound concentration required to reduce the exponential growth of KB cells by 50%, CC50=12 μM
MT-4 cells	Growth inhibition assay			Compound concentration required to reduce the exponential growth of MT-4 cells by 50%, CC50=7.4 μM
Hepa lclc7 cells	Cytotoxicity assay			Cytotoxicity against mouse Hepa lclc7 cells, IC50=39 μM
CHO cells	Cytotoxicity assay			Cytotoxicity against CHO cells by MTT assay, IC50=45.9 μM
MCF7 cells	Function assay			Estrogenic activity in luciferase transfected human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol by luciferase reporter gene assay
NCI-H292 cells	Function assay			Inhibition of PKC-mediated PKD phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis
NCI-H292 cells	Function assay			Inhibition of PKC-mediated p38MAPK phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis
NCI-H292 cells	Function assay			Inhibition of PKC-mediated JNK phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis
dog MDCK cells	Function assay			Restoration of GSH level in dog MDCK cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 24 hrs post-infection by DTNB-based colometric assay
RBL-1 cells	Function assay			In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined, IC50=35 μM
PC3 cells	Cytotoxicity assay			Cytotoxicity against human PC3 cells, IC50=46.4 μM
Hepa-1c1c7 cells	Function assay			Induction of quinone reductase activity in mouse Hepa-1c1c7 cells assessed as drug level required to double enzyme activity
Hepa lclc7 cells	Function assay			Induction of mouse quinone reductase in mouse Hepa lclc7 cells assessed as concentration required to double enzyme activity
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生物活性

製品説明

Isoliquiritigenin, an anti-tumor flavonoid from the root of Glycyrrhiza glabra, inhibits aldose reductase with an IC50 of 320 nM.

Targets

Aldose reductase ^[1]
(Cell-free assay)

320 nM

In Vitro
In vitro	Isoliquiritigenin may be effective in preventing diabetic complications through inhibiting rat lens aldose reductase with an IC50 of 320 nM and sorbitol accumulation in human red blood cells with an IC50 of 2.0 μM. ^[1] This compound may also serve as a skin-lightening agent by inhibiting mono- and diphenolase tyrosinase activities with an IC50 of 8.1 μM. ^[2] It induces cell cycle arrest and cell growth inhibition in DU145 and LNCaP prostate cancer cell lines. ^[3] This chemical induces apoptosis of human gastric cancer MGC-803 cells through increasing the intracellular free calcium concentration and decreasing the mitochondrial transmembrane potential (Deltapsi(m)). ^[4]

In Vivo
In Vivo	LD50: Mice >6g/kg (i.g.) ^[5]

参考
https://pubmed.ncbi.nlm.nih.gov/2118267/ https://pubmed.ncbi.nlm.nih.gov/12590456/ https://pubmed.ncbi.nlm.nih.gov/12706007/ https://pubmed.ncbi.nlm.nih.gov/11731922/ https://pubmed.ncbi.nlm.nih.gov/19067384/ + 展開

参考

https://pubmed.ncbi.nlm.nih.gov/2118267/
https://pubmed.ncbi.nlm.nih.gov/12590456/
https://pubmed.ncbi.nlm.nih.gov/12706007/

https://pubmed.ncbi.nlm.nih.gov/11731922/
https://pubmed.ncbi.nlm.nih.gov/19067384/

+ 展開

化学情報

分子量	256.25	化学式	C₁₅H₁₂O₄
CAS No.	961-29-5	SDF	Download Isoliquiritigenin SDFをダウンロードする
Smiles	C1=CC(=CC=C1C=CC(=O)C2=C(C=C(C=C2)O)O)O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	１ケ月-20°Cin solvent

In vitro
Batch:

Ethanol : 26 mg/mL

DMSO : 12 mg/mL ( (46.82 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

モル濃度計算器

in vivo Batch: Add solvents to the product individually and in order.				投与溶液組成計算機
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.250mg/ml (0.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.500mg/ml (9.76mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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