Ribavirin (ICN-1229)

別名:NSC-163039, RTCA, Tribavirin

Ribavirin (NSC-163039, ICN-1229, RTCA, Tribavirin), a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.

Ribavirin (ICN-1229)化学構造

CAS No. 36791-04-5

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 16900 国内在庫あり
JPY 13600 国内在庫あり
JPY 58200 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール:[email protected]
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カスタマーフィードバック3个实验数据

製品安全説明書

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Ribavirin (ICN-1229)関連製品

Antiviral阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
human KB cells Function assay 1-1000 μg/ml 72 h Antiviral activity against Rhinovirus type 13 infected in human KB cells assessed as inhibition of virus-induced cytopathic effect at 1 to 1000 ug/ml after 72 hrs 211234
MDCK cells Function assay 3 days Antiviral activity against Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis, EC50=0.094 μM 23117173
mouse L1210 cells Function assay 20-60 mins Inhibition of IMPDH in mouse L1210 cells assessed as formation of [2,8-3H]hypoxanthine from [2,8-3H]IMP after 20 to 60 mins, IC50=1 μM 22555152
human CEM cells Function assay 20-60 mins Inhibition of IMPDH in human CEM cells assessed as formation of [2,8-3H]hypoxanthine from [2,8-3H]IMP after 20 to 60 mins, IC50=1 μM 22555152
MDCK cells Function assay 36 h Antiinfluenza activity against influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 36 hrs, IC50=1.9 μM 22341943
MDBK cells Function assay 2 days Antiviral activity against Bovine viral diarrhea virus 1-NADL ATCC VR534 infected in MDBK cells assessed as reduction of viral cytopathic effect after 2 days bt MTS assay, EC50=4.6 μM 22944333
african green monkey Vero cells Function assay 5 days Antiviral activity against RSV A2 infected in african green monkey Vero cells after 5 days by plaque reduction assay, EC50=7 μM 20359898
HEL cells Function assay 2-3 days Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as protection from virus-induced cytopathogenicity after 2 to 3 days by MTT assay, EC50=10 μM 20034711
HEK293 cells Function assay 24 h Inhibition of influenza A virus RNA-dependent RNA polymerase expressed in HEK293 cells after 24 hrs by dual luciferase reporter gene assay, EC50=15 μM 24313730
human BE(2)-C cells Function assay 18-20 h Antiviral activity against Western equine encephalomyelitis virus infected in human BE(2)-C cells assessed as inhibition of viral RNA replication after 18 to 20 hrs by luciferase reporter gene assay, IC50=16 μM 24151954
human MT4 cells Cytotoxic assay 96 h Cytotoxicity against human MT4 cells after 96 hrs by MTT assay, CC50=31 μM 19482481
human HuH7 cells Cytotoxic assay 3 days Cytotoxicity against human HuH7 cells infected with HCV1b after 3 days by MTS assay, CC50=32 μM 22100256
human KB cells Function assay 72 h Antiviral activity against Rhinovirus type 13 infected in human KB cells assessed as inhibition of virus-induced cytopathic effect after 72 hrs relative to control 722730
E6SM cell lines Function assay Effective concentration required to inhibit Herpes simplex virus-1(HSV-1) / thymine kinase deficient (TK-)B2006 / VMW1837 induced cytopathicity by 50% in E6SM cell lines, EC50=0.005 μM 11495586
BHK21 cell line Function assay Inhibition of West Nile virus VLP replicon in BHK21 cell line, EC50=1.1 μM 16539402
HeLa cell Function assay Effective concentration required to inhibit respiratory synaptial(RSV) virus-induced cytopathicity by 50% in HeLa cell lines, EC50=1.5 μM 11495586
human HuH7-J20 cells Function assay Antiviral activity against HCV JFH1 infected in human HuH7-J20 cells assessed as inhibition of viral life cycle by measuring secreted alkaline phosphatase level preincubated with cells for 1 hr followed by viral inoculation for 3 hrs measured at 72 hrs, EC50=30.4 μM 26428870
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生物活性

製品説明 Ribavirin (NSC-163039, ICN-1229, RTCA, Tribavirin), a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.
In Vitro
In vitro Ribavirin significantly reduces the efficiency with which progeny subgenomic replicons transfect new cells in the replicon system, although ribavirin has little effect on levels of HCV replication. Ribavirin increases the mutation frequency of HCV, with the highest rates of mutations being found in the NS5A-encoding region. Ribavirin enhances TH1 while inhibiting T 2 cytokine production by stimulated T cells. [1] Ribavirin shows antiviral activity against a variety of RNA viruses and is used in combination with interferon-alpha to treat hepatitis C virus infection. Ribavirin reduces infectious poliovirus production to as little as 0. 00001% in cell culture. [2] Ribavirin's antiviral activity is exerted directly through lethal mutagenesis of the viral genetic material. [3] Ribavirin markedly reduces viral-induced parameters of macrophage activation at physiologic concentrations (up to 500 mg/mL). Ribavirin inhibits the production of IL-4 by Th2 cells, whereas it does not diminish the production of IFN-gamma in Th1 cells. [4] Ribavirin exhibits antiviral activity against a broad range of both DNA and RNA viruses in vitro. Ribavirin is a cytostatic agent and causes a reduction in synthesis of DNA, RNA and proteins in exposed cells. Ribavirin is thought to induce a switch in T-helper cell phenotype from type 2 to type 1. [5]
実験結果図 Methods Biomarkers 結果図 PMID
Western blot p-AKT / AKT / pBP1 / BP1 Cyclin D1 EZH2 / p-ERK / ERK / p-eIF4E / eIF4E / p21 p53 / p-p53 / p21 / Mdm2 21415224
Growth inhibition assay Cell number 21415224
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05940545 Recruiting
CCHF
Liverpool School of Tropical Medicine
July 12 2023 Phase 1|Phase 2
NCT04283513 Not yet recruiting
Hemorrhagic Fever
U.S. Army Medical Research and Development Command
October 31 2022 Phase 2
NCT04335123 Completed
COVID-19
University of Kansas Medical Center
April 4 2020 Phase 1
NCT04285034 Completed
Lassa Fever
University of Oxford
November 26 2019 --
NCT03889106 Terminated
Lassa Fever
University of Oxford|National Institute for Health Research United Kingdom|Kenema Government Hospital|London School of Hygiene and Tropical Medicine|Public Health England
March 1 2019 --
NCT03585725 Terminated
Follicular Lymphoma|Mantle Cell Lymphoma
Weill Medical College of Cornell University|Memorial Sloan Kettering Cancer Center
September 26 2018 Early Phase 1

化学情報

分子量 244.20864 化学式

C8H12N4O5

CAS No. 36791-04-5 SDF Download Ribavirin (ICN-1229) SDFをダウンロードする
Smiles C1=NC(=NN1C2C(C(C(O2)CO)O)O)C(=O)N
保管

In vitro
Batch:

DMSO : 49 mg/mL ( (200.64 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 49 mg/mL

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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