Durvalumab (anti-PD-L1)

製品コードA2013 別名: MEDI4736

For research use only.
Not for use in humans.

Durvalumab is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.

Durvalumab (anti-PD-L1)
サイズ 価格(税別)
5mg JPY 119300

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(9)

質量管理及び製品安全説明書

PD-1/PD-L1阻害剤の選択性比較

生物活性

製品説明 Durvalumab is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.
同型 Human IgG1
ターゲット
PD-L1/CD80 [1]
(HTRF assay)
PD-1/PD-L1 interaction [1]
(HTRF assay)
0.04 nM 0.1 nM
体外試験

The labeled radioligand shows good affinity to high PD-L1 expression cells and could be blocked with excess unlabeled intact durvalumab.[1]

体内試験

The peak tumor uptake of 124I-Durva-F(ab’)2 was close to 124I-Durva, but much earlier (5.29±0.42% ID/g for 124I-Durva-F(ab’)2 at 12 h vs 5.18±0.73% ID/g for 124I-Durva at 48 h). Compared with 124I-Durva, an accelerated blood clearance was observed for 124I-Durva-F(ab’)2, allowing for a higher tumor-to-background ratio.[1]

お薦めの試験操作(参考用のみ)

細胞試験:
  • The A549 and H460 cells were cultured in six-well plates (~106 cells/well) and divided into two groups. All of the wells were incubated with 5% BSA for blocking nonspecific binding. Group A was presaturated with 100-fold excess of unlabeled durvalumab to block the binding of 124I-Durva-F(ab′)2, and group B had only the medium added. After 30 min, 2 nM of 124I-Durva-F(ab′)2 was added to each group and incubated for 1 h at 37 °C in a cell incubator. After supernatant collection, the cells were lysed using 2 M of NaOH.

動物試験:
  • Animal Models: H460 and A549 tumor-bearing mice
    Dosages: 20 μCi
    Administration: i.v.
    Reference: https://pubmed.ncbi.nlm.nih.gov/35244407/
     

製品説明

CAS No. 1428935-60-7
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

大学・企業名を記入してください
名前を記入してください
電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
お問い合わせ内容をご入力ください

よくある質問(FAQ)

質問:1:
How to store the antibody?

回答:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

Tags: Durvalumab (anti-PD-L1)を買う | Durvalumab (anti-PD-L1) ic50 | Durvalumab (anti-PD-L1)供給者 | Durvalumab (anti-PD-L1)を購入する | Durvalumab (anti-PD-L1)費用 | Durvalumab (anti-PD-L1)生産者 | オーダーDurvalumab (anti-PD-L1) | Durvalumab (anti-PD-L1)化学構造 | Durvalumab (anti-PD-L1)分子量 | Durvalumab (anti-PD-L1)代理店