Fer-1 (Ferrostatin-1)

製品コード：S7243 純度：99.98%

Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.

CAS No. 347174-05-4

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 59500	国内在庫あり
5mg	JPY 22000	国内在庫あり
25mg	JPY 67300	国内在庫あり
100mg	JPY 145500	国内在庫あり
1g	JPY 445500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（404）

製品安全説明書

現在のバッチを見る：純度： 99.98%

99.98

Fer-1 (Ferrostatin-1)と併用されることが多い化合物

RSL3

Ferrostatin-1 and RSL3 combination significantly inhibit NCX4040 cytotoxicity in colorectal cancer (CRC) cells, HT-29/HCT 116.

Sinha BK, et al. Cells. 2023 Jun 14;12(12):1626.

Z-VAD-FMK

Ferrostatin-1 and Z-VAD-FMK combination block the cell viability inhibited by CAY10566 in ovarian cancer cell lines OVCAR-5.

Zhao G, et al. Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2203480119.

Obeticholic Acid (INT-747)

Ferrostatin-1 and Obeticholic acid combination significantly reduce inflammation in non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) disease.

Li S, et al. Front Pharmacol. 2022 Dec 16;13:1081553.

Chloroquine

In the presence of nanoplatform, Ferrostatin-1 has no effect on cell growth, while Chloroquine alleviates growth inhibition in H460/A549 cells.

Liu R, et al. J Exp Clin Cancer Res. 2023 Jul 29;42(1):188.

Cisplatin

Ferrostatin-1 and Cisplatin combination are used to rescue selenium-induced eye defects.

Gao M, et al. Int J Mol Sci. 2022 Apr 26;23(9):4783.

Fer-1 (Ferrostatin-1)関連製品

関連製品	Erastin RSL3 Liproxstatin-1 Deferoxamine mesylate FIN56 IKE(Imidazole ketone erastin) UAMC-3203 iFSP1 Ammonium ferric citrate SRS16-86 SRS11-92	もっと見る
関連化合物ライブラリー	Metabolism Compound Library Anti-cancer Metabolism Compound Library Glutamine Metabolism Compound Library Carbohydrate Metabolism Compound Library Lipid Metabolism Compound Library	もっと見る

Ferroptosis阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
HRE cells	Cell viability assay	1 μM	48-72 hours	Induction of cell apoptosis	30794682
U57810 and C2C12 cells	Cell viability assay	10 μM	24, 48, and 72 h	Administration of ferrostatin-1 almost completely prevented the ferroptotic cell death.	29971532
human IMR32 neuroblastoma cells	Function assay		13 h	Antiferroptotic activity in human IMR32 neuroblastoma cells assessed as inhibition of erastin-induced cell death preincubated for 1 hr before erastin stimulation and measured after 13 hrs by fluorescence plate reader method, IC50=0.018 μM	26696014
Leishmania major Friedlin clone V1	Antileishmanial assay		72 h	LD50 = 28.75 μM	26410073
IMR32	Function assay		1 h	IC50 = 0.033 μM	30354101
HT1080 cells	Function assay			Antiferroptotic activity in human HT1080 cells assessed as inhibition of erastin-induced cell death by Alamar blue assay, IC50=0.095 μM	26696014
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.

Targets

Ferroptosis ^[1]
(HT-1080 cells)

60 nM(EC50)

In Vitro
In vitro	Ferrostatin-1 (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. Ferrostatin-1 is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. Ferrostatin-1 does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. ^[1]
細胞実験	細胞株	HT-1080 cells
	濃度	60 nM (EC50)
	反応時間	24 h
	実験の流れ	Erastin treated cells were incubated with ferrostatin-1 at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	Collagen I / β-actin KIM-1 / NGAL / β-actin gp91 phox / β-actin Nrf2 / Gpx4 / β-Actin cleaved caspase-3 / β-actin		33679620
	Growth inhibition assay	Cell viability Cell viability		31571665
	Immunofluorescence	ROS formation renal apoptosis morphological features of HT-22 cells γH2AX		33679620

In Vivo
In Vivo	Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.^[2]
動物実験	動物モデル	C57BL/6 mice
	投与量	10 mg/kg
	投与経路	i.n.

参考
[1] Dixon SJ, et al. Cell, 2012, 149(5), 1060-1072.. [2] Tuo QZ, et al. Signal Transduct Target Ther. 2022 Feb 23;7(1):59.

参考

化学情報

分子量	262.35	化学式	C₁₅H₂₂N₂O₂
CAS No.	347174-05-4	SDF	Download Fer-1 (Ferrostatin-1) SDFをダウンロードする
Smiles	CCOC(=O)C1=CC(=C(C=C1)NC2CCCCC2)N
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 55 mg/mL ( (209.64 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 55 mg/mL

Water : Insoluble

モル濃度計算器

in vivo Batch: Add solvents to the product individually and in order.					投与溶液組成計算機
	Clear solution	5% DMSO 1 40% PEG 300 2 5%tween 80 3 50%ddH2O	1.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O Dilute to 1 mL. The mixed solution should be used immediately for optimal results.	投与溶液組成計算機
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	1.65mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	2% DMSO 1 50% PEG 300 2 5% Tween 80 3 ddH2O	1.0mg/ml	Taking the 1 mL working solution as an example, add 20 μL of 50 mg/ml clarified DMSO stock solution to 500 μL PEG300, mix evenly to clarify it; add 50 μL Tween-80 to the above system, mix evenly to clarify it; Then continue to add 430 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Handling Instructions

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* 必須

C1=C0/X	C1:	LOG(C1):
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C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):