Obinutuzumab (anti-CD20)

製品コードA2023 別名: Gazyva, GA101, Gazyvaro, Afutuzumab

For research use only.
Not for use in humans.

Obinutuzumab (Gazyva, GA101, Gazyvaro, Afutuzumab) (anti-CD20) is a novel, type II, glycoengineered, humanized anti-CD20 monoclonal antibody that has been developed for lymphocytic leukemia and lymphoma of B-cell origin.

Obinutuzumab (anti-CD20)
サイズ 価格(税別)
5mg JPY 142900

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

質量管理及び製品安全説明書

CD markers阻害剤の選択性比較

生物活性

製品説明 Obinutuzumab (Gazyva, GA101, Gazyvaro, Afutuzumab) (anti-CD20) is a novel, type II, glycoengineered, humanized anti-CD20 monoclonal antibody that has been developed for lymphocytic leukemia and lymphoma of B-cell origin.
体外試験

GA101 is the first Fc-engineered, type II humanized IgG1 antibody against CD20. Relative to rituximab, GA101 has increased direct and immune effector cell-mediated cytotoxicity and exhibits superior activity in cellular assays and whole blood B-cell depletion assays.[3]

体内試験

In human lymphoma xenograft models, GA101 exhibits superior antitumor activity, resulting in the induction of complete tumor remission and increased overall survival. In nonhuman primates, GA101 demonstrates superior B cell–depleting activity in lymphoid tissue, including in lymph nodes and spleen.[3]

お薦めの試験操作(参考用のみ)

細胞試験:
  • Objective: Annexin V/PI FACS assay
    Cells: NHL cell lines
    Concentrations: 10 μg/mL
    Incubation Time: 36 hr
    Method: For CD40 stimulation of hCD20 cells from transgenic mice, red blood cells (RBCs) are removed by hypotonic ammonium-chloride-potassium (ACK) lysis, and untouched splenic B cells are purified by anti-CD43–mediated depletion of non-B cells (MACS). B cells are incubated in conditioned complete RPMI 1640 medium containing 10% fetal bovine serum for 2 hours (with or without 10 μg/mL mitogenic stimulation) and either anti-CD40 or anti-IgM Fab2 antibodies. This is followed by 36 hours of anti-CD20 stimulation (GA101 or rituximab 10 μg/mL). As positive controls for the induction of cell death, the following agents are used: 100nM staurosporine for mitochondrial-mediated apoptosis, or 1 μg/mL anti-CD95 Fas for the extrinsic induction of apoptosis.
    Reference: https://pubmed.ncbi.nlm.nih.gov/20194898/

    Obinutuzumab can apply to humanized mice (eg:SCID beige mice), peripheral blood and other related assays (Only for Reference)

動物試験:
  • Objective: Tumor control ratio (TCR) and tumor growth inhibition (TGI)
    Animal Models: 4- to 8-week-old female SCID beige mice
    Formulation: --
    Dosages: 30 mg/kg
    Administration: i.p.
    Reference: https://pubmed.ncbi.nlm.nih.gov/24304419/

    Objective: NHL xenograft studies in SCID beige mice
    Animal Models: 4 to 5-week-old female SCID beige mice
    Formulation: --
    Dosages: 10 mg/kg, 30 mg/kg
    Administration: i.p.
    Reference: https://pubmed.ncbi.nlm.nih.gov/20194898/

    Obinutuzumab can apply to humanized mice (eg:SCID beige mice), peripheral blood and other related assays (Only for Reference)

製品説明

CAS No. 949142-50-1
Formulation PBS buffer, pH 7.2
Isotype Humanized IgG1
Source Humanized (from mouse)
Storage Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

大学・企業名を記入してください
名前を記入してください
電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
お問い合わせ内容をご入力ください
Tags: Obinutuzumab (anti-CD20)を買う | Obinutuzumab (anti-CD20) ic50 | Obinutuzumab (anti-CD20)供給者 | Obinutuzumab (anti-CD20)を購入する | Obinutuzumab (anti-CD20)費用 | Obinutuzumab (anti-CD20)生産者 | オーダーObinutuzumab (anti-CD20) | Obinutuzumab (anti-CD20)化学構造 | Obinutuzumab (anti-CD20)分子量 | Obinutuzumab (anti-CD20)代理店