Plerixafor (AMD3100)

製品コード:S8030 別名:JM 3100, SID791

For research use only.

Plerixafor (AMD3100, JM 3100, SID791) is a chemokine receptor antagonist for CXCR4 and CXCL12-mediated chemotaxis with IC50 of 44 nM and 5.7 nM in cell-free assays, respectively. Plerixafor inhibits human immunodeficiency virus (HIV) replication.

Plerixafor (AMD3100)化学構造

CAS No. 110078-46-1

サイズ 価格(税別)
JPY 18100
JPY 26400
JPY 80000

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(82)

製品安全説明書

CXCR阻害剤の選択性比較

相関CXCR製品

生物活性

製品説明 Plerixafor (AMD3100, JM 3100, SID791) is a chemokine receptor antagonist for CXCR4 and CXCL12-mediated chemotaxis with IC50 of 44 nM and 5.7 nM in cell-free assays, respectively. Plerixafor inhibits human immunodeficiency virus (HIV) replication.
ターゲット
CXCL12 [1]
(Cell-free assay)
CXCR4 [1]
(Cell-free assay)
5.7 nM 44 nM
体外試験

Plerixafor inhibits CXCL12-mediated chemotaxis with a potency lightly better than its affinity for CXCR4. [1] Plerixafor also antagonizes SDF-1/CXCL12 ligand binding with an IC50 of 651 nM. Plerixafor inhibits SDF-1 mediated GTP-binding, SDF-1 mediated calcium flux and SDF-1 stimulated chemotaxis with IC50 of 27 nM, 572 nM and 51 nM, respectively. Plerixafor does not inhibit calcium flux against cells expressing CXCR3, CCR1, CCR2b, CCR4, CCR5 or CCR7 when stimulated with their cognate ligands, nor does Plerixafor inhibit receptor binding of LTB4. Plerixafor does not, on its own, induce a calcium flux in the CCRF–CEM cells, which express multiple GPCRs including CXCR4, CCR4 and CCR7. [2]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHOK1 NGnab3NHfW6ldHnvckBie3OjeR?= NG[2OlJFcXOybHHj[Y1mdnRib3[gX|EzPUmfU1TGNYFteGijIH\yc40hS0OUMj;DXGNTPCCneIDy[ZN{\WRiaX6gR2hQUzFiY3XscJMtKEmFNUCgQUAxNjByMEC0JO69VS5? M{jjV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{G1NVI5Lz5zN{exOVEzQDxxYU6=
CHOK1 MoT2SpVv[3Srb36gZZN{[Xl? NWXYTmxWTGm|cHzhZ4Vu\W62IH;mJHsyOjWLXV3DVFEh\nKxbTDDR3IzN0O[Q2K0JIV5eHKnc4Pl[EBqdiCFSF;LNUBk\WyuczygTWM2OCB;IECuNFAxODlizszNMi=> NUO4N3dpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NVUyOjhpPkG3O|E2OTJ6PD;hQi=>
CHOK1 M2rseWZ2dmO2aX;uJIF{e2G7 NUfndWZ7TGm|cHzhZ4Vu\W62IH;mJHsyOjWLXWPESlFidHCqYTDmdo9uKEO[Q2K0JIV5eHKnc4Pl[EBqdiCFSF;LNUBk\WyuczygTWM2OCB;IECuNFAxQDFizszNMi=> NWXqW4tjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NVUyOjhpPkG3O|E2OTJ6PD;hQi=>
U87.CD4 MVTBcpRqfmm{YXygZZN{[Xl? M1HOUmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTly0N{Bqdm[nY4Tl[EBqdiCXOEeuR2Q1KGOnbHzzJIV5eHKnc4PpcochcHWvYX6gR3hEWjRiSEK4NWEhdXW2YX70MEBKSzVyIE2gNE4xODF7IN88UU4> NV3BTJBHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
U87.CD4 MULBcpRqfmm{YXygZZN{[Xl? MmXsRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQRFugbY5n\WO2ZXSgbY4hXTh5LlPEOEBk\WyuczDlfJBz\XO|aX7nJIh2dWGwIFPYR3I1KEh{OEHBJI12fGGwdDygTWM2OCB;IECuNFAyQSEQvF2u MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
MT4 NGH5eoJCdnSrdnnyZYwh[XO|YYm= NFHzTpI2KGSjeYO= NGH6W4FCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCjZoTldkA2KGSjeYOgZpkhVVSWIHHzd4F6NCCHQ{WwJF0hOC5yMEKg{txONg>? NWrkdYpIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5O|Q{PzZpPkK2PVc1Ozd4PD;hQi=>
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U87.CD4 NGDvTFlCdnSrdnnyZYwh[XO|YYm= M1TDcGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiY3zpcolk[WxiaYPvcIF1\SBzMDDpcoZm[3SnZDDpckBWQDdwQ1S0JINmdGy|IHX4dJJme3OrbnegbJVu[W5id3ns[EB1gXCnIFPYR3I1NCCLQ{WwJF0hOC5yMEOg{txONg>? MoXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
PBMC NIf3e|ZHfW6ldHnvckBie3OjeR?= NGm0fZVG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBi\2GrboP0JGhKXi1zIEi5MlYhe3S{YXnuJIlvKFCETVOgZ4VtdHNuIFXDOVAhRSByLkCwN|gh|ryPLh?= NFnDfnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNE[5PFE5QSd-MUS2PVgyQDl:L3G+
MT4 NV62S4hqSW62aY\pdoFtKGG|c3H5 NV\JWJJqPCCmYYnz M3rDNWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNicnXwcIlk[XSrb36gZYZ1\XJiNDDkZZl{KGK7IF3UWEBie3OjeTygSWM2OCB;IECuNFA1KM7:TT6= NV[0WXdRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwOFM3OzhpPkKwNFQ{PjN6PD;hQi=>
MT-4 M4DwOmZ2dmO2aX;uJIF{e2G7 M3O0V2Vn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiSFnWMVEpUUmLQjmgdoVxdGmlYYTpc44hcW5iTWStOEBk\WyuczygSWM2OCB;IECuNFA1OiEQvF2u NYriNYVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEW2PFc6Pyd-OEW2PFc6PzxxYU6=
HEK293 NYHKfFU6SW62aY\pdoFtKGG|c3H5 MXOyJIRigXN? NX7kfGJUSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQuOyCrbn\lZ5Rm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjCyJIRigXNuIFnDOVAhRSByLkCwOFYh|ryPLh?= M{[0dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEWxN|A2Lz5zOUS1NVMxPTxxYU6=
HEK293 MnvlRY51cX[rcnHsJIF{e2G7 MXSyJIRigXN? MX7BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDteYx1cWS{dXegdoV{cXO2YX70JGhKXjFiSGjCNkBqdm[nY4Tl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB{IHThfZMtKEmFNUCgQUAxNjByNUOg{txONg>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
MT-4 MUPGeY5kfGmxbjDhd5NigQ>? NEDiUIdG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IFjJWk0zMFKRRDmgdoVxdGmlYYTpc44hcW5iTWStOEBk\WyuczygSWM2OCB;IECuNFA2QSEQvF2u MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTZ6N{m3K|45PTZ6N{m3QE9iRg>?
CEM-CCRF M2niZmZ2dmO2aX;uJIF{e2G7 M1TVPFMxKG2rboO= M1zuRmlvcGmkaYTpc44hd2ZiUFWtZ49vcnWpYYTl[E0yOkd3IHHueIkuS1iFUkSgZY51cWKxZImgZolv\GmwZzD0c{BEYEOUNDDpckBpfW2jbjDDSW0uS0OURjDj[YxteyCycnXpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliYX70bYJw\HliYXTkbZRqd25iYomgSmFEWyCFYX70c{BKUSCleYTv[ox2d3KxbXX0dolkKGGwYXz5d4l{NCCLQ{WwJF0hOC5yME[g{txONg>? M4KwbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUexNFM5Lz5{N{W3NVA{QDxxYU6=
HEK293 NG\ESoxCdnSrdnnyZYwh[XO|YYm= MmPUNkBl[Xm| NVvqSJU6SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDIXGIzKGmwZnXjeIVlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEKg[IF6eyxiSVO1NEA:KDBwMEC2NkDPxE1w M3vidVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEWxN|A2Lz5zOUS1NVMxPTxxYU6=
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アッセイ
Methods Test Index PMID
Immunofluorescence CXCR4 ; β-arrestin2 28521261 31186083
体内試験 A single topical application of Plerixafor promotes wound healing in diabetic mice by increasing cytokine production, mobilizing bone marrow EPCs, and enhancing the activity of fibroblasts and monocytes/macrophages, thereby increasing both angiogenesis and vasculogenesis. [3] Cohorts of mice are administered with PBS, IGF1, PDGF, SCF, or VEGF for five consecutive days and Plerixafor on the 5th day. The number and size of the colonies are highest in IGF1 plus Plerixafor injected mice compared to PDGF, SCF and VEGF treated groups, in combination with Plerixafor. [4]

お薦めの試験操作(参考用のみ)

動物試験:[4]
  • 動物モデル:Twelve-week-old C57BL/6 mice with segmental bone defect
  • 投薬量:5 mg/kg
  • 投与方法:Administered via i.p.

溶解度 (25°C)

体外

化学情報

分子量 502.78
化学式

C28H54N8

CAS No. 110078-46-1
Storage 3年 -20°C
2年 -80°C in solvent
Smiles C1CNCCNCCCN(CCNC1)CC2=CC=C(C=C2)CN3CCCNCCNCCCNCC3

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05421416 Not yet recruiting Drug: Loratadine|Drug: Placebo Stem Cell Transplant Complications AHS Cancer Control Alberta November 1 2022 Phase 2
NCT05343572 Not yet recruiting Drug: Plerixafor Asherman Syndrome|Atrophic Endometrium|Recurrent Implantation Failure Hugh Taylor|Yale University September 2022 Early Phase 1
NCT05411575 Recruiting Drug: Plerixafor 20 MG/ML [Mozobil]|Other: Placebo COVID-19 Acute Respiratory Distress Syndrome|COVID-19 4Living Biotech|4P-Pharma July 19 2022 Phase 2
NCT05445128 Recruiting Biological: MGTA-145|Drug: Plerixafor Sickle Cell Disease Magenta Therapeutics Inc.|bluebird bio June 24 2022 Phase 2
NCT04646603 Recruiting Drug: MRG-001|Drug: Placebo COVID-19|ARDS Human|Stem Cells|Regeneration MedRegen LLC|ICON plc|Johns Hopkins University December 1 2021 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

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よくある質問(FAQ)

質問1:
How about the half-life of the product (Cat S8030)?

回答
The biological half-life for this drug is 3-5 hours: https://en.wikipedia.org/wiki/Plerixafor.

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