Thiamine HCl (Vitamin B1)

製品コード：S3211 純度：99.99%

Thiamine or vitamin B1 is a water-soluble vitamin of the B complex, its phosphate derivatives are involved in many cellular processes.

CAS No. 67-03-8

サイズ	価格(税別)	在庫状況
50mg	JPY 18100	国内在庫あり
200mg	JPY 43700	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

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Thiamine HCl (Vitamin B1)関連製品

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Vitamin阻害剤の選択性比較

生物活性

製品説明	Thiamine or vitamin B1 is a water-soluble vitamin of the B complex, its phosphate derivatives are involved in many cellular processes.

In Vitro
In vitro	Thiamine (50 mM), in addition to its nutritional value, induces systemic acquired resistance (SAR) in rice, tobacco, cucumber, and Arabidopsis. Thiamine-treated rice, Arabidopsis (Arabidopsis thaliana), and vegetable crop plants shows resistance to fungal, bacterial, and viral infections. Thiamine treatment induces the transient expression of pathogenesis-related (PR) genes in rice and other plants. In addition, Thiamine treatment potentiates stronger and more rapid PR gene expression and the up-regulation of protein kinase C activity. ^[1] Vitamin B1 (10 μM) prevents acetaldehyde-induced inhibition of myocyte shortening in adult rat ventricular myocytes. Vitamin B1 (10 μM) effectively blunts the acetaldehyde-induced depression in ±dL/dt in adult rat ventricular myocytes. Vitamin B1 (10 μM) prevents acetaldehyde-induced shortening of time-to-peak shortening in adult rat ventricular myocytes. Vitamin B1 (10 μM) prevents acetaldehyde-induced elevation in both protein carbonyl formation and caspase-3 activation in adult rat ventricular myocytes. ^[2] Thiamine uptake is energy- and temperature-dependent, pH-sensitive, Na+-independent, saturable at both the nanomolar (apparent Km, 30 nM) and the micromolar (apparent Km, 1.72 mM) concentration ranges in ARPE-19 cells. Uptake of Thiamine is adaptively regulated by extracellular substrate level via transcriptionally mediated mechanisms that involve both hTHTR-1 and hTHTR-2 in ARPE-19 cells, it is also regulated by an intracellular Ca2+-calmodulin-mediated pathway. ^[3] Thiamine-responsive megaloblastic anemia (TRMA) fibroblasts are rescued from death with 10 nM-30 nM Thiamine (in the range of normal plasma thiamine concentrations). Normal fibroblasts exhibits saturable, high-affinity thiamine uptake (Km 400 nM-550 nM; Vmax 11 pmol/min/1×10⁶ cells), while TRMA fibroblasts lacks detectable high-affinity uptake. At 30 nM Thiamine, the rate of uptake of Thiamine by TRMA fibroblasts is 10-fold less than that of wild-type, which explains the increased apoptosis of TRMA fibroblasts. ^[4]
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	THICL / THICS / THI4L / THI4M / THI4S / ACT Bax / Bid / p53 / p84 Rpb1 / Fcp1 / f-Rpb3 / Tfg3 / Rpb7 / Rpb4		18093957
	Growth inhibition assay	TEN1 and TEAEN1 strains hippocampal Dcx+ cells		19028995
	Glycolysis stress test	Glycolysis stress		29914147
	Cell death	Cell survival		31193162
	Western blot / RNA Blot	PR-1 / rRNA PR-1 / rRNA		15980201
	Immunofluorescence	Thi76KR / Thi7M399R / ThiN350K		31658248

In Vivo
In Vivo	Thiamine deficiency results in amyloid precursor protein immunoreactivity accumulated in swollen neurites within, or around lesions in rats, or in abnormal clusters in mice. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06057597	Recruiting	Obesity\|Morbid	Assistance Publique - Hôpitaux de Paris	November 13 2023	Not Applicable
NCT05840718	Not yet recruiting	Septic Shock	Hospital Sao Domingos	September 2023	--
NCT05474105	Recruiting	Ischemic Stroke	Barts & The London NHS Trust\|Queen Mary University of London\|Nutricia Research	July 11 2023	Not Applicable
NCT04505878	Withdrawn	Primary Graft Dysfunction\|Lung Transplant; Complications	University of Wisconsin Madison	April 2023	Phase 2

参考
[1] Ahn IP, et al. Plant Physiol, 2005, 138(3), 1505-1515. [2] Aberle NS 2nd, et al. Alcohol Alcohol, 2004, 39(5), 450-454. [3] Subramanian VS, et al. J Physiol, 2007, 582(Pt 1), 73-85. [4] Stagg AR, et al. J Clin Invest, 1999, 103(5), 723-729. [5] Calingasan NY, et al. Neuroreport, 1997, 8(11), 2631-2634. + 展開

参考

+ 展開

化学情報

分子量	337.27	化学式	C₁₂ H₁₇N₄OS.HCl
CAS No.	67-03-8	SDF	Download Thiamine HCl (Vitamin B1) SDFをダウンロードする
Smiles	CC1=C(SC=[N+]1CC2=CN=C(N=C2N)C)CCO.Cl.[Cl-]
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

Water : 67 mg/mL

DMSO : Insoluble ( 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Handling Instructions

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* 必須

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