Vedolizumab (anti-α4β7-integrin)

製品コードA2045 別名: Entyvio

For research use only.
Not for use in humans.

Vedolizumab (anti-α4β7-integrin) is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.

Vedolizumab (anti-α4β7-integrin)
サイズ 価格(税別)
5mg JPY 127200
25mg JPY 377600

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

質量管理及び製品安全説明書

Integrin阻害剤の選択性比較

生物活性

製品説明 Vedolizumab (anti-α4β7-integrin) is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.
体外試験

Vedolizumab (anti-α4β7-integrin) binds to a subset of human peripheral blood memory CD4+ T lymphocytes including gut-homing interleukin 17 T-helper lymphocytes at the highest level, also binds to eosinophils at high levels, and to naive T-helper lymphocytes, naive and memory cytotoxic T lymphocytes, B lymphocytes, natural killer cells, and basophils at lower levels; Vedolizumab binds to memory CD4+ T and B lymphocytes with subnanomolar potency with EC50 of 0.3-0.4 nM.[1]

お薦めの試験操作(参考用のみ)

細胞試験:
  • PBMCs were resuspended in assay buffer for CD4 memory cell interleukin (IL) 17A intracellular staining. PBMCs were stimulated (or not stimulated, for the control samples) with 50 ng/ml phorbol 12-myristate 13-acetate and 1 µg/ml Ionomycin in the presence of 3 µM Monensin for 5 h at 37°C. After washing, cells were stained with fluorescent-labeled antibodies: vedolizumab, anti-α4 and anti-β7, anti-CD4, and anti-CD45RO, where applicable, at 4°C for 30 min. Cells were then washed with cold PBS and resuspended in fixation and permeabilization working solution staining buffer at 4°C for 30 min. After fixation and permeabilization, cells were washed with permeabilization buffer and blocked with 10% normal rat serum at 4°C for 10 min. After the blocking step, cells were stained with fluorochrome-conjugated anti_x0002_IL17A antibody at 4°C for 30 min. Finally, cells were washed with permeabilization buffer and analyzed.

製品説明

CAS No. 943609-66-3
Molecular Weight 145.7 KD
Formulation PBS Buffer, PH 7.4
Isotype Human IgG1
Application Vedolizumab (anti-α4β7-integrin)
Storage Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles.
Sterility 0.2 μM filtered

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