Wnt-C59 (C59)


Wnt-C59 (C59)化学構造


Wnt-C59 (C59) is a PORCN inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase with IC50 of 74 pM in HEK293 cells.

サイズ 価格(税別)  
JPY 43000

お探しのディーラーが見当たらない場合は直接こちらのメールアドレスまでお問い合わせください:[email protected]



  • Application of XAV939 or Wnt-c59 with FGF2 blocks the accumulation of nuclear b-catenin in MGPCs, and increases levels of p38MAPK in Muller glia, whereas levels of cFos are unaffected. a, b, Eyes were injected with four consecutive daily injections of FGF2 6 XAV939 or Wnt-c59, BrdU 24 h after the last injection of FGF2, and retinas harvested 24 h later. Wnt-c59 (treated). a, b-catenin in the nuclei of Sox91 Muller glia/MGPCs on 70% grayscale background.

    Dev Neurobiol, 2016, 76(9):983-1002. . Wnt-C59 (C59) purchased from Selleck.

  • In vitro treatment with C59 (Wnt-i) decreased the active (non-phosphorylated) β-catenin level in Eed cKO primary rib chondrocytes. Chondrocytes were cultured for 24 h in the presence of C59 (Wnt-i).

    Nat Commun, 2016, 7:12047. Wnt-C59 (C59) purchased from Selleck.

  • Migration of L428 cells (b) and KM-H2 (c) after 72 h pretreatment with 5 μm Wnt-C59 or DMSO with (+CM) or without (−CM) the simultaneous stimulation with the respective cHL-CM for 24 h (mean±s.d., n=3, one-way ANOVA and Dunn’s post hoc test). Note the inhibition of migration by porcupine inhibitors, which is rescued by CM. (d) Invasion of L428 or KM-H2 cells after 72 h pretreatment with 5 μm Wnt-C59 or DMSO (mean±s.d., n=3 two-way ANOVA and Bonferroni’s post hoctest). (e) Adhesion of DMSO- or Wnt-C59-pretreated L428 cells on either HUVECs (white) or collagen I (gray) (mean±s.d., n=5, 2-way ANOVA and Bonferroni’s post hoc test). Note the decrease of adhesion after WNT inhibition (*P<0.05 and ***P<0.001).

    Oncogene, 2017, 36(1):13-23. Wnt-C59 (C59) purchased from Selleck.

  • (A) Body weight changes over the study by treatment group (LGK low: LGK974 at 3 mg/kg/day; LGK high: LGK974 at 6 mg/kg/kg/day; C59: Wnt-C59). (B) Femur length at necropsy. (C) Total body bone mineral density (BMD) at necropsy. (D) Spine BMD at necropsy.

    J Endocrinol, 2018, 238(1):13-23. Wnt-C59 (C59) purchased from Selleck.

  • (B) Wnt3a, β-catenin, and cyclin D1 expression in hAMSCs was determined by western blotting. hAMSCs were pre-treated with or without Wnt-C59 (100 nM), 1 h before HA (0.6 mg/ml, 36 h) treatment. hAMSCs lysates were then prepared and subjected to western blotting using antibodies specific to wnt3a, β-catenin, and cyclin D1. Quantification graphs (relative density) reflected the intensity of respective proteins to β-actin. Data are shown as means±S.D. (n=3). Control culture received medium alone. *p<0.05, **p<0.01 vs control. #p<0.05, ##p<0.01 vs HA group.

    Exp Cell Res, 2016, 345(2):218-29.. Wnt-C59 (C59) purchased from Selleck.




製品説明 Wnt-C59 (C59) is a PORCN inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase with IC50 of 74 pM in HEK293 cells.
Porcn [1]
(HEK293 cells )
74 pM

Wnt-C59 (C59) is claimed to inhibit PORCN enzyme activity at nanomolar concentrations. Wnt-C59 (10 nM) blocks the palmitoylation-dependent Wnt–WLS interaction in HeLa cells transfected with either WNT3A-V5 or WNT8A-V5 plasmids. Wnt-C59 (100 nM) prevents incorporation of palmitate into WNT3A in HeLa cells transfected with WNT3A-V5, consistent with inhibition of PORCN activity. Wnt-C59 (100 nM) inhibits the activity of all splice variants of murine PORCN in PORCN-null HT1080 cells transfected with PORCN. Wnt-C59 is a nanomolar inhibitor of mammalian PORCN acyltransferase activity and blocks activation of all evaluated human Wnts. Wnt-C59 does not significantly inhibit the proliferation of any of 46 tested cancer cell lines in vitro at concentrations that completely inhibit PORCN. [1] Wnt-C59 is capable to significantly inhibit proliferation and comparable to the ICG-001 treated NMuMG (NMG) cells. Wnt-C59 inhibits sphere formation by threefold in NMuMG (NMG) cells, which is dependent on Wnt10b-secretion. Wnt-C59 inhibits proliferation of human MDA-MB 231 cells by >50%. [2] Wnt-C59 (a Porcupine inhibitor) blocks radiolabel incorporation of [125I]iodo-pentadecanoate in mouse L-Wnt3a cells transfected with Flag-Porcupine. [3]

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HT1080 cells M{XySWZ2dmO2aX;uJIF{e2G7 MXXJcohq[mm2aX;uJI9nKHCxcnP1dIlv\SCjY4Tpeol1gSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCKVEGwPFAh[2WubIOgZZN{\XO|ZXSgZZMhe3WycILld5Nqd25ib3[gW451O0FvbXXkbYF1\WRic4Xw[ZIhfG:yIH\sZZNpKGGldHn2bZR6KGK7IGPUSkBtfWOrZnXyZZNmKGG|c3H5MEBKSzVyPUeuOIUuODVizszNMi=> Mnz0NlY2OjJ7NE[=


Methods Test Index PMID
Western blot
β-catenin / pPKC; 

PubMed: 26941358     

Western blotting analysis in cortical neurons on day 18. Each WNT inhibitor inhibited Wnt/β-catenin-dependent signaling during the SFEBq differentiation procedure, but C59 also inhibited Wnt/β-catenin independent signaling.

Cyclin D1; 

PubMed: 31156379     

C59 treatment significantly decreased the levels of cyclin D1 mRNA (0.66 ± 0.032, *p < 0.05 compared to control levels). However, exogenous Wnt3a prevented this decrease and recovered cyclin D1 mRNA to the levels in control cells, which were significantly different than those in C59-treated cells (1.16 ± 0.032, **p < 0.01).

Growth inhibition assay
Cell proliferation; 

PubMed: 25980501     

Cell proliferation influenced by concentrations of Wnt-C59 treatment. Wnt-C59 has a clear inhibitory effect on the growth of HK1 cells.

Cell viability; 

PubMed: 31156379     

Cytotoxicity was evaluated through assessment of mitochondrial functionality with an MTT assay. Treatment with C59 for 24 h at concentrations over 100 μM reduced SH-SY5Y vitality, causing a mortality rate of up to 40% at the 200 μM concentration (0.6 ± 0.011, ***p < 0.001). Although a slight decrease in SH-SY5Y vitality was observed at 100 μM, this difference was not significant.

25980501 31156379
体内試験 Wnt-C59 concentration remains greater than 10-fold above the in vitro IC50 for at least 16 hours following a single oral dose (5 mg/kg) in mice. Wnt-C59 (10 mg/kg) prevents growth of MMTV-WNT1 tumors in female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors. Wnt-C59 (10 mg/kg) decreases Wnt pathway activity and decreased proliferation in MMTV-WNT1 tumors in female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors as evident by decreased expression of β-catenin target gene expression. [1] Wnt-C59 (10%) topically administered 4 weeks decreases the size of dysplasia of SmoM2-expressing cells in adult K14CREER/Rosa–SmoM2 mice. [4]




+ 展開
  • 細胞株: NMuMG (NMG) and MDA-MB 231 cells
  • 濃度: --
  • 反応時間: 48 hours
  • 実験の流れ:

    Seed cells at a concentration of 4×103/well in 100 μL culture medium containing various amouts of Wnt-C59. Incubate cells for 48 hours at 37 ℃. Add 10 μL/well cell proliferation reagent and incubate for 4 hours at 37 ℃. Shake thoroughly for 1 min on a shaker. Measure the absorbance of the samples in a microplate (ELISA) reader.



+ 展開
  • 動物モデル: Female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors
  • 製剤: 0.5% methylcellulose and 0.1% Tween-80
  • 投薬量: 10 mg/kg
  • 投与方法: orally

溶解度 (25°C)

体外 DMSO 76 mg/mL (200.28 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。


分子量 379.45


CAS No. 1243243-89-1
in solvent
別名 N/A





質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)


  • 質量





開始濃度 x 開始体積 = 最終濃度 x 最終体積


この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1



  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):




チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2


質量 濃度 体積 分子量



Handling Instructions


  • * 必須


  • 質問1:

    I want to use Wnt-C59 for i.p. injection, can you advise how to dissolve it?

  • 回答:

    S7037 Wnt-C59 can be dissolved in 2% DMSO+30% PEG 300+5% Tween 80+ddH2O at 5 mg/ml as a clear solution. After stayed for a while, the precipitation will go out. So it is recommended to prepare the solution just before use.



Tags: Wnt-C59 (C59)を買う | Wnt-C59 (C59) ic50 | Wnt-C59 (C59)供給者 | Wnt-C59 (C59)を購入する | Wnt-C59 (C59)費用 | Wnt-C59 (C59)生産者 | オーダーWnt-C59 (C59) | Wnt-C59 (C59)化学構造 | Wnt-C59 (C59)分子量 | Wnt-C59 (C59)代理店
細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID