Nicotinamide (NSC 13128)

製品コード:S1899 別名:Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452

For research use only.

Nicotinamide (NSC 13128, Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

Nicotinamide (NSC 13128)化学構造

CAS No. 98-92-0

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 23500
JPY 18100

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(26)

製品安全説明書

Sirtuin阻害剤の選択性比較

相関Sirtuin製品

生物活性

製品説明 Nicotinamide (NSC 13128, Niacinamide, Vitamin PP, Nicotinic acid amide, Vitamin B3, NSC 27452), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.
ターゲット
Sirtuin [1]
体外試験

Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. [1] Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells. [2] Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha). [3] Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability. [4] Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. [5]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BL21 (DE3) NV7nblJNTnWwY4Tpc44h[XO|YYm= NX;3cWRtUW6qaXLpeIlwdiCxZjDjZZRidHm2aXPhcIx6KGGldHn2[UBpfW2jbjDTTXJVOyBqMUCyJJRwKDN7OTDhcYlvdyCjY3nkd{kh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUApTEV|KTDj[YxteyC3c3nu[{BndHWxcn;n[Y5q[yB5LXHtbY5wNTRvbXX0bJlt[2:3bXHybY4hMEGPQzmtcIFj\WynZDDw[ZB1cWSnIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3ME22MlLPxE1w NXrjNHBERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO|U5OjRpPkK1Nlc2QDJ2PD;hQi=>
BL21 (DE3) NVHGdZF5TnWwY4Tpc44h[XO|YYm= MoLrTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiCVSWLUNkBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJEhFTTNrIHPlcIx{KHW|aX7nJIZtfW:{b3flcolkKDdvYX3pco8uPC2vZYTofYxkd3WvYYLpckApSU2FKT3sZYJmdGWmIIDldJRq\GViYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyPUGwMlXPxE1w NG\xVI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3OVgzPCd-MkWyO|U5OjR:L3G+
BL21(DE3) MmW5SpVv[3Srb36gZZN{[Xl? NWe3Nmd1UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDzeYJ{fHKjdHWg[IVi[2W2eXzheIlwdiC3c3nu[{BbVUGOIHHzJJN2[nO2cnH0[UwhUUN3ME2yN{4y|ryPLh?= M3vifVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNEm2NlUyLz5|MUS5OlI2OTxxYU6=
SK-MEL-28 NF;HZ4lHfW6ldHnvckBie3OjeR?= NEXvVFgyODBidV2= M4CxdVI1KGi{cx?= NHjyb|dT\WS3Y4Tpc44hcW5iQWTQJIxmfmWuIHnuJIh2dWGwIGPLMW1GVC1{ODDj[YxteyCjdDCxNFAhfU1ibXHpcpRicW6nZDDpckBNd2OtZTfzJJNwdHW2aX;uJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlMYJie2WmIHHzd4F6KGmwIHHid4Vv[2Vib3[gS2Yh[W6mIHfseYNwe2V? Mor2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{W3NVkoRjJ{OEO1O|E6RC:jPh?=
A673 NVjqc|JNeUiWUzDhd5NigQ>? NG\1VXRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC Mn;BdWhVWyCjc4PhfS=> MUDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{ NWjwRnFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1 Mn7MdWhVWyCjc4PhfS=> NHnoWnZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBPSi2HQnOxJINmdGy| MkHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 MV3xTHRUKGG|c3H5 NYDOTWd2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BL21(DE3) NXHVS4tITnWwY4Tpc44h[XO|YYm= NEfJeIFKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JHNkcGm|dH;zc41iKG2jboPvcokhe2m{dIXpckAzKCh{MTD0c{A{OjJicnXzbYR2\XNrIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFqRFWzLUBk\WyuczDpckBxemW|ZX7j[UBw\iCcTVHM NH;DVGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUS5OlI2OSd-M{G0PVYzPTF:L3G+
BL21(DE3) Mly5SpVv[3Srb36gZZN{[Xl? NXXqSJd[UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiKGqpMUhDfXRrTInzMWFOSw>? NXX1cppwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G0PVYzPTFpPkOxOFk3OjVzPD;hQi=>
BL21(DE3) MWTGeY5kfGmxbjDhd5NigQ>? NY\KOopLUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBU[2irc4Tvd49u[SCvYX7zc45qKHOrcoT1bY4hOiBqMkGgeI8hOzJ{IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzKFTFN{kh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiKGqpMUhJ\XhrTInzMWFOSw>? NWHpUFltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G0PVYzPTFpPkOxOFk3OjVzPD;hQi=>
BL21(DE3) NH6zZo9HfW6ldHnvckBie3OjeR?= Mkn0TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDTZ4hqe3Sxc3;tZUBu[W6|b37pJJNqenS3aX6gNkApOjFidH:gN|IzKHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyMESHMzmgZ4VtdHNiaX6gdJJme2WwY3Wgc4YhMFprLTjPZ5QqVHm|LVHNRy=> M{PEW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNEm2NlUyLz5|MUS5OlI2OTxxYU6=
HEK293T MYrGeY5kfGmxbjDhd5NigQ>? MWG2NEBucW6| NV75Z45CUW6qaXLpeIlwdiCxZjDTeJJmeFSjZz30ZYdo\WRiaIXtZY4hW0GUTUGgWGlTKCh3NkGgeI8hPzJ2IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hUEWNMkmzWEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iTlHEZZNmKGGldHn2bZR6KGmwIIDy[ZNmdmOnIH;mJG5CTCtiaX7jeYJifGWmIH\vdkA3OCCvaX7zJIJ6KEiSTFOgZY5idHm|aYOsJGlEPTB;NEOuPO69VS5? NYDVSHBkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF2MD:nQmNpTU2ETEyvZV4>
アッセイ
Methods Test Index PMID
Western blot p-MLC / MLC / p-MYPT1 / MYPT1 ; p-β-catenin / β-catenin ; Sp1 / ERK 30503259 18446063
Immunofluorescence p-MLC 30503259
体内試験 In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%[6].

お薦めの試験操作(参考用のみ)

細胞試験:

[7]

  • 細胞株:HaCaT cells
  • 濃度: 33 μM
  • 反応時間:7 days and 14 days
  • 実験の流れ:

    The established cell line of human epidermal keratinocytes (HaCaT cells) was routinely cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum and kept in a humidified atmosphere containing 5% CO2 at 37°C. For NAD(P) modulation, cells were grown in DMEM and 10% dialyzed fetal bovine serum and with addition of 33 μM nicotinamide (33 μM Nam) or without added nicotinamide (0 μM Nam). Cell number was measured by counting. Sensitivity to glutaminase inhibition was performed using 0.1 μM 6-diazo-5-oxo-L-norleucine (DON) on cells grown in 33 μM Nam or 0 μM Nam for 7 days.

動物試験:

[6]

  • 動物モデル:Mice (strain CBA/Ht/GyfBSVS)
  • 投薬量:100, 200, 300 and 500 mg/kg
  • 投与方法:i.p.

溶解度 (25°C)

体外

化学情報

分子量 122.12
化学式

C6H6N2O

CAS No. 98-92-0
Storage 3年 -20°C
2年 -80°C in solvent
Smiles C1=CC(=CN=C1)C(=O)N

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05449249 Not yet recruiting Procedure: Long-term fasting Healthy|Periodontitis Buchinger Wilhelmi Development & Holding GmbH|Buchinger Wilhelmi Clinic|University of Geneva Switzerland|King''s College London|ETH Zurich (Switzerland) September 1 2022 Not Applicable
NCT05298410 Recruiting Other: Limitless Hypertension Limitless Research Inc.|KGK Science Inc. March 2022 Phase 2
NCT05040321 Recruiting Drug: MIB-626|Drug: Placebo Alzheimer''s Disease (Incl Subtypes)|Dementia Brigham and Women''s Hospital December 1 2021 Phase 1|Phase 2
NCT04763499 Recruiting Other: freeze dried strawberry powder Vascular Dilation University of California Davis December 1 2021 Not Applicable
NCT04641663 Enrolling by invitation Dietary Supplement: Multi-target Dietary Supplement (MTDS) Aging|Age-related Cognitive Decline Douglas Boreham|McMaster University|Northern Ontario School of Medicine September 3 2021 Not Applicable
NCT04844528 Recruiting Drug: Nicotinamide|Drug: Placebo Chronic Lymphocytic Leukemia University of Utah August 5 2021 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

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