PubMed: 30987343      Viruses

Effective delayed chain termination of RNA synthesis by remdesivir. (A) Remdesivir-MP-dependent delayed chain termination of RNA synthesis was studied with purified EBOV RdRp. RNA synthesis was monitored in the presence of [α-32P]GTP, CTP, and ATP or remdesivir-TP, supplemented with increasing concentrations of UTP for incorporation at position i+6, representing a 12-nt RNA product labeled 12.

PubMed: 33835389      Virol Sin

Thirty-six hours post-transfection, Western blot for tGFP-BlaR expression is carried out with remdesivir treated cells.

PubMed: 32793908      bioRxiv

(C) Remdesivir alone increased ACE2 protein expression. H1299 human NSCLC cells, MSTO-211H human mesothelioma cells, and Calu-3 human NSCLC type II alveolar cells were treated with remdesivir and VS-6766 for 48 hours. ACE2 was probed with Abnova PAB13444 antibody, and β-Actin was probed with Sigma A5441 antibody as a loading control.

PubMed: 32595613      Front Microbiol

(C) Western blot of EV71 VP2 protein expression in HeLa cells. HeLa cells were infected with EV71 (MOI = 0.5). After adsorption, the cells were treated with the indicated concentrations of remdesivir and collected at 24 hpi. Then, the proteins were resolved by SDS-PAGE and western blotting.

PubMed: 33835389      Virol Sin

293 T cells are transfected with SARS-CoV-2-GFP replicon, remdesivir is added to culture media 10 h post transfection or just before transfection respectively, the green fluorescence intensity is determined thirty-six hours post-transfection, and dose responsive curves are drawn and EC50 value of remdesivir (B) was calculated by four-parameter nonlinear regression respectively.

PubMed: 33186749      Cell Mol Gastroenterol Hepatol

Remdesivir inhibits SARS-CoV-2 infection of PSC-HIOs. (B) Effect of remdesivir on metabolic activity of Caco-2 cells was assessed by CellTiter-Glo assay. Shown are technical triplicates from one representative experiment. Error bars represent SEM.

PubMed: 32595613      Front Microbiol

(B) Cell viability after culture with different doses of remdesivir at 24 hpi assessed using a CCK8 assay (mean ± SD, n = 6).

PubMed: 32589775      Clin Transl Sci

Plasma concentration‐vs‐time profiles following RDV single‐dose administration; mean (±SD) values are plotted. LLOQ, lower limit of quantification; RDV, remdesivir.

PubMed: 33782830      Clin Pharmacokinet

Mean (standard deviation) of remdesivir (RDV) plasma concentration vs time by dose following 30-min intravenous infusion(s) of a single 200-mg dose or multiple 100-mg remdesivir doses in healthy subjects.

PubMed: 33376043      Phytomedicine

Dose-dependent inhibition of MERS-CoV infection by lycorine. (B) The confocal microscope images showed cell nuclei (red) and MERS-CoV spike (S) protein (green) at the indicated lycorine concentration or 8.3 μM remdesivir (RDV) after MERS-CoV infection. Scale bar = 100 μM.

PubMed: 33186749      Cell Mol Gastroenterol Hepatol

(C) PSC-HIOs were infected with SARS-CoV-2 and treated with indicated concentrations of remdesivir. Two days later, infection was analyzed by viral S protein and E-caherin (Ecad, green) staining. Nuclei are stained with DAPI in blue. Scale bar = 50 μM. Images are representative for at least 3 independent experiments.

PubMed: 33173719      Adv Sci (Weinh)

Evaluation of potential antiviral efficacy of remdesivir on the chip system. B) Confocal immunofluorescent microscopy images of epithelium (E‐cadherin) and endothelium (VE‐cadherin) of alveolus chip treated without or with 1 × 10−6 m remdesivir at day 2 post‐infection.

PubMed: 32869028      bioRxiv

Antiviral SARS-CoV-2 activity and cytotoxicity of PF-00835231, and remdesivir in A549+ACE2 cells. f. Representative images of SARS-CoV-2 USA-WA1/2020 syncytia formation at 48 hpi in A549+ACE2 cells under treatment with 0.33 μM PF-00835231, or remdesivir.

PubMed: 32595613      Front Microbiol

(D) Fluorescence microscopy examination of EV71-infected HeLa cells treated with different amounts of remdesivir (24 hpi). The dsRNA-specific antibody J2 (red) was used for staining viral dsRNA replication intermediates, and nuclei were labeled with Hoechst 33258 (blue). Bar = 100 μm.

PubMed: 32516797      Nature

Viral loads and virus titers in swabs collected from rhesus macaques infected with SARS-CoV-2 and treated with remdesivir. Panel A shows viral loads and Panel B shows infectious virus titers in nose, throat and rectal swabs collected daily from animals treated with remdesivir (n=6) or vehicle solution (n=6). Statistical analysis was performed using a 2-way ANOVA with Sidak’s multiple comparisons test.