Bafilomycin A1(Baf-A1)
製品コードS1413
分子量(MW)：622.83
Bafilomycin A1 is a vacuolar H+-ATPase inhibitor with IC50 of 0.44 nM.
質量管理及び製品安全説明書
Proton Pump阻害剤の選択性比較
生物活性
| 製品説明 | Bafilomycin A1 is a vacuolar H+-ATPase inhibitor with IC50 of 0.44 nM. | ||||||||||||||||||||||||||||||||||||||||||||
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| 体外試験 |
Bafilomycin A1 is a toxic macrolide antibiotic derived from Streptomyces griseus. Bafilomycin A1 inhibits the short circuit current induced by the outer mantle epithelium (OME). The IC50 and maximum inhibition dose of Bafilomycin A1 are 0.17 μM and 0.5 μM, respectively. [2] In addition, Bilomycin A1 inhibits the acid influx with an IC50 value of 0.4 nM. Bafilomycin A1 inhibits the acidification dose-dependently resulting in a lower quenching, and thus a higher fluorescence. [3] Bafilomycin A1 prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. [4] Bafilomycin A1 also affects the transport of endocytosed material from early to late endocytic compartments. Bafilomycin not only dissipates the low endosomal pH but also blocks transport from early to late endosomes in HeLa cells. [5] Bafilomycin A1 at doses of 0.1-1 μM completely inhibits the acidification of lysosomes revealed by the incubation with acridine orange in BNL CL.2 and A431 cells. [6] When Bafilomycin A1 is added to Hanks' balanced salt solution, endogenous protein degradation is strongly inhibited and numerous autophagosomes accumulated in H-4-II-E cells. Bafilomycin A1 also prevents the appearance of endocytosed HRP in autophagic vacuoles. [7] |
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| 体内試験 | Bafilomycin A1 (1 μM and 0.1 μM) completely inhibits the resorptive activity of cultured osteoclasts. [8] Bafilomycin A1 dose-dependently inhibits the rate of Na+ uptake in young tilapia with a Ki of 0.16 μM. [9] |
お薦めの試験操作（参考用のみ）
| キナーゼ試験： |
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ATPase enzyme activity assays: The ATPase enzyme assay medium contains 6 mM MgSO4, 50 mM HEPES (pH 7.4), 200 mM Na2SO3 (V-ATPase activator), 0.5 mM sodium ortho-vanadate (P-ATPase inhibitor), 0.5 mM sodium azide (F-ATPase inhibitor) and 3 mM Na2ATP. This medium (1.0 mL), with or without the addition of the V-type ATPase inhibitor bafilomycin A1, is incubated with the filtered homogenate (0.1 mL) for 60 minutes at 23–25 °C. The reaction is stopped by the addition of 1 mL of TCA 3%. Spectrometric blanks are prepared as for the enzyme assay with the exception that the tissue sample is added after the acid. Phosphate analysis is accomplished by adding 2 mL of 1-butanol and 0.2 mL molybdate solution (5 g ammonium molybdate, 22 mL H2SO4 to 100 mL). After vortexing for 15 seconds the solution is neutralised with 0.5 mL citrate solution (100 g/500 mL, pH 7.0) and again vortexed for 15 seconds. The solution is then centrifuged (2000 × g; 3 minutes) to separate the butanol phase and the absorbance of this phase is read at 400 nm. Standards of orthophosphate are prepared (0.1 μM–2.0 μM) and treated in the same way as the enzyme activity assays. Enzyme activity is expressed in μmol of orthophosphate liberated per hour and per milligram of protein. V-ATPase activity is considered to be the difference between the total ATPase activity measured in the presence of Na2SO3, sodium orthovanadate and sodium azide and the ATPase activity measured in the presence of these reagents and of the specific V-ATPases inhibitor Bafilomycin A1. |
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| 細胞試験： |
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| 動物試験： |
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参考
- [1] al-Fifi ZI, et al. Insect BiochemMol Biol, 1998, 28(4), 201-211.
- [2] Oliveira PF, et al. Comp Biochem Physiol A Mol Integr Physiol, 2004, 139(4), 425-432.
- [3] S?rensen MG, et al. J Bone Miner Res, 2007, 22(10), 1640-1648.
- [4] Papini E, et al. Mol Microbiol, 1993, 7(2), 323-327.
- [5] Bayer N, et al. J Virol, 1998, 72(12), 9645-9655.
- [6] Yoshimori T, et al. J Biol Chem, 1991, 266(26), 17707-17712.
- [7] Yamamoto A, et al. Cell Struct Funct, 1998, 23(1), 33-42.
- [8] Sundquist KT, et al. J Bone Miner Res, 1994, 9(10), 1575-1582.
- [9] Fenwick JC, et al. J Exp Biol, 1999, 202 Pt 24, 3659-3666.
- [10] Kanzawa T, et al. Cell Death Differ, 2004, 11(4), 448-457.
溶解度 (25°C)
| 体外 | DMSO | 6 mg/mL (9.63 mM) |
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| Water | Insoluble | |
| Ethanol | Insoluble |
* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。
化学情報
| 分子量 | 622.83 |
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| 化学式 | C35H58O9 |
| CAS No. | 88899-55-2 |
| 保管 | 粉 |
| in solvent | |
| 別名 | N/A |
便利ツール
モル濃度計算器
求めたい質量、体積または濃度を計算してください。
質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)
モル濃度計算器方程式
*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA（製品ページで利用可能な）。
希釈計算器
貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます：
開始濃度 x 開始体積 = 最終濃度 x 最終体積
希釈の計算式
この方程式は、一般に略語を使われます：C1V1 = C2V2 ( 入力 出力 )
常に貯蔵液を準備するとき、小びんラベルとMSDS/COA（オンラインで利用できる）で見つかる製品のバッチに特有の分子量を使ってください。
分子量计算器
そのモル質量と元素組成を計算するために、合成物の化学式を入力してください：
チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2
モル濃度計算器
技術サポート
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よくある質問（FAQ）
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質問1：
How to dissolve it？
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回答：
S1413 Bafilomycin A1(Baf-A1) is soluble in DMSO at 0.1 mg/ml. Please do not use alcohols as solvent, because this compound will degrade in alcohols.
