Vismodegib (GDC-0449)

製品コード:S1082

For research use only.

Vismodegib (GDC-0449) is a potent, novel and specific hedgehog inhibitor with IC50 of 3 nM and also inhibits P-gp with IC50 of 3.0 μM in a cell-free assay.

Vismodegib (GDC-0449)化学構造

CAS No. 879085-55-9

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 25900
JPY 16900
JPY 30200
JPY 63400
JPY 156100

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(173)

製品安全説明書

Hedgehog/Smoothened阻害剤の選択性比較

相関Hedgehog/Smoothened製品

生物活性

製品説明 Vismodegib (GDC-0449) is a potent, novel and specific hedgehog inhibitor with IC50 of 3 nM and also inhibits P-gp with IC50 of 3.0 μM in a cell-free assay.
ターゲット
Hedgehog [1]
(Cell-free assay)
3 nM
体外試験

GDC-0449 targets the Hedgehog signaling pathway, blocking the activities of the Hedgehog-ligand cell surface receptors PTCH and/or SMO and suppressing Hedgehog signaling. GDC-0449 prevents multiple ATP-binding cassette (ABC) transporters. GDC-0449 also blocks ABCG2, Pgp, and MRP1-important ABC transporters associated with MDR. GDC-0449 is a potent inhibitor of ABC transporters, ABCG2/BCRP and ABCB1/Pgp, and is a mild inhibitor of ABCC1/MRP1. In ABCG2-overexpressing HEK293 cells, GDC-0449 increases retention of the fluorescent ABCG2 substrate BODIPY-prazosin and resensitizes these cells to mitoxantrone. In Madin-Darby canine kidney II cells engineered to overexpress Pgp or MRP1, GDC-0449 increases the retention of calcein-AM and resensitizes them to colchicine. GDC-0449 also resensitizes human non-small cell lung carcinoma cells NCI-H460/par and NCI-H460/MX20, which overexpress ABCG2 in response to mitoxantrone, to mitoxantrone, and to topotecan or SN-38. The IC50 values of GDC-0449 for prevention of ABCG2 and Pgp are about 1.4 μM and 3.0 μM, respectively. [2] GDC-0449 alters intracellular Ca2+ homeostasis and inhibits cell growth in cisplatin-resistant lung cancer cells. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
IGROV-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moq3TWM2OD1yLkC3NlQ5KM7:TR?= MmW4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
HCE-T MmHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUP6NmhHUUN3ME2xMlMzOjR5IN88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
D-542MG NGHNbIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTFwOE[3N|ch|ryP NIHteoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
23132-87 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjNW4lKSzVyPUSuOFAyPDdizszN MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
HDLM-2 NF25bFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRThwMES3OlYh|ryP NUfRWZdORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
ACN Mkn3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWL2U5NoUUN3ME24MlUxOTB7IN88US=> MnPOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
HuO-3N1 MlLHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjsXG5UUUN3ME25MlYxOTB6IN88US=> NE\0NJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
BHT-101 NFPMdG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrUTYVjUUN3ME2xNU4{QCEQvF2= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
KYSE-150 NHLrZ|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoS4TWM2OD1zMT61PFQyKM7:TR?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
MC-IXC MoDuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPnWmRKSzVyPUGyMlIzQTJizszN NF7BU3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
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NY M13jdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT5SFFsUUN3ME2xOE45QTB|IN88US=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
HOS M17US2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFftO5lKSzVyPUG1MlY4OTlizszN M1zOcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
NB7 MmTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TON2lEPTB;MUWuPFkyKM7:TR?= M3j1fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
DMS-273 MnrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVX5Zm1zUUN3ME2xOk43PzF|IN88US=> Ml3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
MDA-MB-361 M1XuNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXP5bGFNUUN3ME2xO{4zPzFzIN88US=> M3m1fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
DU-145 NF36dFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\LZmlEPTB;MUiuN|Ih|ryP MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
NCI-H82 M2f6RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{Ti[mlEPTB;MUmuPFM5PiEQvF2= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLLZ2N3UUN3ME2yNE4xOTl4IN88US=> Mm\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
GCT MnLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrSUYlKSzVyPUKwMlg5OjRizszN Mly4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
C2BBe1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrmTWM2OD1{MT6xNFU5KM7:TR?= Mo\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
LB2241-RCC Mnj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLkTWM2OD1{MT64OFQyKM7:TR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
COLO-829 NUXCdmw6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIO1VHRKSzVyPUKyMlE5PzFizszN M4izRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
EW-11 Ml\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XncmlEPTB;MkKuPFAzOiEQvF2= M2eyXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
NCI-H526 Mln2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4n5NWlEPTB;MkOuOFcyPyEQvF2= MkHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
SF295 M3PMc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M124d2lEPTB;MkSuNFI2OiEQvF2= NUDLWIV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
D-566MG MoHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTJ3LkK5OFMh|ryP NV;zWFFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
8505C MkjsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV2yT2Z1UUN3ME2yOU43OzNzIN88US=> NETvRpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
HT-29 M3\pWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrieY5vUUN3ME2yOk4xPDNzIN88US=> NE\qeJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
NBsusSR MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTJ4LkiwNFYh|ryP NVvhRYVURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
BV-173 NHz3[XVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTjcYpKSzVyPUK4MlMyQDJizszN MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
CTB-1 M3vsNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPnVG1oUUN3ME2zNE4yODNzIN88US=> NFW0VGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
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CAMA-1 NYHYPZlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;ab2J1UUN3ME2zN{41PjF3IN88US=> M3fVXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
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A172 NHzjd3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHnTWM2OD1|Nz60PVIyKM7:TR?= NWnkZopCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
QIMR-WIL MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVGzRmR7UUN3ME2zPE4xPzB6IN88US=> NIC5TGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
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ONS-76 MlvnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPz[ohKSzVyPUSzMlMxPTdizszN NHzzfpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
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NOS-1 M1LXW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkW5TWM2OD12ND62NFMyKM7:TR?= M4DlcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
A101D NELMV|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfaTWM2OD12ND64NFI{KM7:TR?= M1LBN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
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Calu6 NIrXW2JHfW6ldHnvckBie3OjeR?= NIntU3I1KGi{cx?= NGDZOZJRdGG|bXGgZ49v[2WwdILheIlwdiCrbjDoeY1idiCFYXz1OkBk\WyuczD4[Y5w\3KjZoTl[EBqdiCwdXTlJI1wfXOnIHH0JFc2KG2pL3vnMEBxdyCkaXSgcYVie3W{ZXSgZYZ1\XJiNDDodpMheG:|dDDmbYZ1cCCmb4PlMEBEeCB;IEKzJO69VS5? M{XLOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{G2Nlk3Lz5zOUexOlI6PjxxYU6=
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LS180 NUTmUlkxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M3[zWlczKGi{cx?= MlfKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOU{G4NEBk\WyuczDh[pRmeiB5MjDodpMh[nlic4Xs[o9zcG:mYX3pcoUuSiCjc4PhfUwhUUN3MDC9JFQ2KM7:TT6= NXS4[VZ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwPVkzPTdpPkOwNFk6OjV5PD;hQi=>
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BxPC3 NEXoTo9CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHjqSlQ4OiCqcoO= MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEK6UFOzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIx2dWmwZYPj[Y51KGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iNEeuPVUh|ryPLh?= NHfMRo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkiyNFU2PCd-Mk[4NlA2PTR:L3G+
medulloblastoma cell MVXBcpRqfHWvb4KgZZN{[Xl? MVPBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCvZXT1cIxw[myjc4TvcYEh[2WubIOgfIVvd2e{YX\0[YQhcW5iUITjbEAsNy1ibX;1d4Uh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6geJVud3Jidn;seY1mKGG2IEGyMlUhdWdxa3esJJBwKGKrZD6= M1fRU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{G2Nlk3Lz5zOUexOlI6PjxxYU6=
S12 MkTlSpVv[3Srb36gZZN{[Xl? NWXCTpZyPDhiaILz M2T3fGlvcGmkaYTpc44hd2ZiaIXtZY4hW0iKIIDheIh4[XliaX6gcY92e2ViU{GyJINmdGy|IHHzd4V{e2WmIHHzJGdNUS2vZXTpZZRm\CC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSCrbjDwdoV{\W6lZTDv[kAyKG2pL33MJIh2dWGwIHHsdIhiNTFvYXPp[EBodHmlb4Dyc5RmcW5w MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR|OEWyO{c,OjF2M{i1Nlc9N2F-
Shh Light2 NYXNbHlrTnWwY4Tpc44h[XO|YYm= NGi5dJQxNjFidV2= NXj0[IJ6OzZiaILz M4LaRWlvcGmkaYTpc44hd2ZiU33vMY1m\GmjdHXkJGhpKHOrZ37hcIxqdmdicHH0bJdigSCrbjDtc5V{\SCVaHigUIlocHR{IHPlcIx{KGG|c3Xzd4VlKGG|IIP1dJBz\XO|aX;uJI9nKEeuaUGtcXJPSSCneIDy[ZN{cW:wIHH0JFAvOSC3TTDt[YF{fXKnZDDh[pRmeiB|NjDodpMh[nliUmStdXBEWiCjbnHsfZNqey5? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd|NkC2N{c,Ojd5M{[wOlM9N2F-
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HCT116 NV72dHdbSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MnrqNVAhfU1? NYXrT5ZJPDhiaILz NETySVFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjdDCxNEB2VSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6Ng>? NH\nUlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5NVQ2QSd-MkS0PVE1PTl:L3G+
NIH/3T3 NH;NRW9HfW6ldHnvckBie3OjeR?= MVixNEB2VQ>? NGnQdlNKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGG|c3Xzd4VlKGG|IHTve44hemWpdXzheIlwdiCxZjDtVHREUDFibWLORUBmgHC{ZYPzbY9vKGG2IEGwJJVONg>? MoTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2OUG0OVkoRjJ2NEmxOFU6RC:jPh?=
MSC NIXoW4FHfW6ldHnvckBie3OjeR?= M33hblIxKHSxIEiwJJVO MWrJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCrbjDtc5V{\SCPU1OgZ4VtdHNiYYPz[ZN{\WRiYYOg[I94diC{ZXf1cIF1cW:wIH;mJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGG2IEKwJJRwKDhyIIXNMi=> NWftVmVlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PVE1PTlpPkK0OFkyPDV7PD;hQi=>
SK-N-MC MYTxTHRUKGG|c3H5 M3u1XZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzzMi=> MnrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 M1fBSpFJXFNiYYPzZZk> MYrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzzMi=> NV\qTZB7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NIH3T3-Luc Ml;tSpVv[3Srb36gZZN{[Xl? MUSxJJVO MXG0PEBpenN? NEj5epFKdmirYnn0bY9vKG:oIIPtc{1u\WSrYYTl[EBp\WSpZXjv[{B{cWewYXzpcocheGG2aIfhfUBqdiCvb4Xz[UBPUUh|VEOtUJVkKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaTDtVm5CKGW6cILld5Nqd25iYYSgNUB2VSCjZoTldkA1QCCqcoOgZpkhemWjbD30bY1mKFCFUjDhcoFtgXOrcz6= M32ycVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEm5OFg{Lz5{OUS5PVQ5OzxxYU6=
C3H10T1/2 MlXaSpVv[3Srb36gZZN{[Xl? NV;NXXpsOjRiaILz MVfJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6NW2nZHnheIVlKEeOSUGgcXJPSSCneIDy[ZN{cW:wIHnuJI1wfXOnIFOzTFExXDFxMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nzJIlvKHC{ZYPlcoNmKG:oIDixVkw{[VJuNGOsO4FTMS15YT3t[ZRpgWxvMT2oLHIqNTZvbXX0bJltcGWydHHuMVIugWxrb3P0ZYh6\HKxLUHIMYlv\GWwLUStfYwhOy2qeXTyc5h6[mWwen;heIUv MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd|MEm4OEc,OjR5M{C5PFQ9N2F-
C3H10T1/2 NH3VemxHfW6ldHnvckBie3OjeR?= M4HnW|I1KGi{cx?= NVG0d2JnUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeT3t[YRq[XSnZDDHUGkyKG2UTlGg[ZhxemW|c3nvckBqdiCvb4Xz[UBEO0hzMGSxM|Ih[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kBXTDNw NXfkPJUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|A6QDRpPkK0O|MxQTh2PD;hQi=>
NIH/3T3 Mne4SpVv[3Srb36gZZN{[Xl? MX6xNFAhdk1? M1PMbVM3KGi{cx?= NXLhdlZyUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGG|c3Xzd4VlKGG|IHTve45z\We3bHH0bY9vKG:oIGPobE1qdmS3Y3XkJGdtcTFibWLORUBmgHC{ZYPzbY9vKGyndnXsd{BifCBzMECgcm0h[W[2ZYKgN|YhcHK|IHL5JHJVNXGSQ2KgZY5idHm|aYOu NYf0N2MzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4O|M{ODNpPkK4PFc{OzB|PD;hQi=>
C3H10T1/2 M3zSbmZ2dmO2aX;uJIF{e2G7 MYexNFAhdk1? M3S3e|M3KGi{cx?= NXTuOHNzUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDDN2gyOFRzL{KgZ4VtdHNiYYPz[ZN{\WRiYYOg[I94dnKnZ4XsZZRqd25ib3[gV4hpNWmwZIXj[YQhT2yrMTDtVm5CKGW6cILld5Nqd25ibHX2[Yx{KGG2IEGwNEBvVSCjZoTldkA{PiCqcoOgZpkhWlRvcWDDVkBidmGueYPpd{4> NXnqWlA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4O|M{ODNpPkK4PFc{OzB|PD;hQi=>
NIH/3T3 Mlj2SpVv[3Srb36gZZN{[Xl? MUOxNFAhdk1? Mo\DN|YhcHK| MlyxTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKFOqaD3pcoR2[2WmIID0Z4gyKG2UTlGg[ZhxemW|c3nvckBt\X[nbIOgZZQhOTByIH7NJIFnfGW{IEO2JIhzeyCkeTDSWE1yWEOUIHHuZYx6e2m|Lh?= M2rJWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEezN|A{Lz5{OEi3N|MxOzxxYU6=
C3H10T1/2 MlTqSpVv[3Srb36gZZN{[Xl? MnPRNVAxKG6P MVezOkBpenN? Mn;JTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiU3joMYlv\HWlZXSgdJRkcDFibWLORUBmgHC{ZYPzbY9vKGyndnXsd{BifCBzMECgcm0h[W[2ZYKgN|YhcHK|IHL5JHJVNXGSQ2KgZY5idHm|aYOu Mn\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6N{OzNFMoRjJ6OEezN|A{RC:jPh?=
medulloblastoma cell MnfIRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1;CdZVxKHSxIEOwJJVO NFrHRWY4OiCqcoO= NHTuV4dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIGD0Z4gsNy1icEWzMU8uKG2nZIXscI9jdGG|dH;tZUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEBxem:uaX\ldoF1cW:wIIXwJJRwKDNyIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmu MnSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6N{OzNFMoRjJ6OEezN|A{RC:jPh?=
medulloblastoma cell MWXBdI9xfG:|aYOgZZN{[Xl? NXHFZ|B3UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBud3W|ZTDQeINpMy9vIIC1N{0wNSCvZXT1cIxw[myjc4TvcYEh[2WubIOgbY1xdGGwdHXkJIlvKGG2aIntbYMhdnWmZTDtc5V{\SCjdDCxNk42KG2pL3vnMEBqeCCkaXSg[o9zKDF3IHPvcpNm[3W2aY\lJIRigXNiYomgWHVPTUxiYYPzZZkv MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh5M{OwN{c,Ojh6N{OzNFM9N2F-
NIH/3T3 MVnGeY5kfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGW6cILld5Nm\CCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gbIVl\2Wqb3etbY5lfWOnZDDHcIkuOiCjY3P1cZVt[XSrb36gZZQhfGmyIH;mJJBzcW2jcomgZ4ltcWFiYomgSGFRUSC|dHHpcolv\yCkYYPl[EBkd26ob3PhcEBucWO{b4Pjc5Bq[yCjbnHsfZNqey5? M3nnUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEGwOVkyLz5{N{ixNFU6OTxxYU6=
NIH/3T3 MULGeY5kfGmxbjDhd5NigQ>? MlTrTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gbIVl\2Wqb3etbY5lfWOnZDDTcY8uTUeIUDDjbYxq[XK7IITyZY5{dG:lYYTpc44h[nliRFHQTUB{fGGrbnnu[{Bj[XOnZDDjc45nd2OjbDDtbYNzd3Olb4DpZ{BidmGueYPpd{4> NYjtdlNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke4NVA2QTFpPkK3PFExPTlzPD;hQi=>
medulloblastoma cell MoDLRY51cS22dX3vdkBie3OjeR?= M1PVVmFvfGlvdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBud3W|ZTDQeINpMy9vIIC1N{0wNSCvZXT1cIxw[myjc4TvcYEh[2WubIOgbY1xdGGwdHXkJIlvKGG2aIntbYMhdnWmZTDtc5V{\SCjc4Pld5Nm\CCjczD0eY1weiCpcn;3eIghcW6qaXLpeIlwdiCjdDCyNEBu\y:tZzygdI8h[WSvaX7pd5RmemWmIIT3bYNmKGSjaXz5JJZq[SCpYY\h[4UhdWWjc4Xy[YQh\X[ncomgc5Rp\XJiZHH5JIR2emmwZzDjc41xd3WwZDDkc5Nqdmdw Mn3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5M{m3NVQoRjJ7N{O5O|E1RC:jPh?=
C3H10T1/2 NVHGTmJTTnWwY4Tpc44h[XO|YYm= MVS0MlEhfG9iMUCwNEBvVQ>? Mn6xRYxtd3O2ZYLpZ{BqdmirYnn0bY9vKG:oIGPtc{BqdiCvb4Xz[UBEO0hzMGSxM|Ih[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDweZJud3KyaHHtbY5mNWmwZIXj[YQhT2yrMTD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgOE4yKHSxIEGwNFAhdk1iYomgdXBEeiCjbnHsfZNqey5? M1rUNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEe0OVQyLz5{M{C3OFU1OTxxYU6=
C3H10T1/2 MXvGeY5kfGmxbjDhd5NigQ>? MXi0MlEhfG9iMUCwNEBvVQ>? MljTR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDTcY8hcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV2FINWmwZIXj[YQhT2yrMTD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgOE4yKHSxIEGwNFAhdk1iYomgdXBEWiCjbnHsfZNqey5? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
アッセイ
Methods Test Index PMID
Western blot Bcl-2 / Bax ; p53 / Cyclin D1 / p21 ; Gli1 / SOX2 / OCT4 ; p-GSK3β / GSK3β / p-Akt / Akt / Gli1 ; Fas / DR4 / DR5 / Cleaved PARP / Bcl-2 / Cleaved caspase-3 / PDGFRα 28195165 26418365 27143997 22087285
Immunofluorescence Gli1 / Gli2 22087285
Growth inhibition assay Cell viability 29042665
体内試験 GDC-0449 has been used to treat medulloblastoma in animal models. [2] GDC-0449 prevents the growth of primary pancreatic xenografts without non-specifically inhibiting pancreatic cell proliferation. Oral dosing of GDC-0449 causes tumor regressions in the Ptch(+/-) allograft model of medulloblastoma at doses ≥25 mg/kg and tumor growth inhibition at doses up to 92 mg/kg dosed twice daily in two ligand-dependent colorectal cancer models, D5123, and 1040830. Analysis of Hh pathway activity and PK/PD modeling reveals that GDC-0449 inhibits Gli1 with a similar IC50 in both the medulloblastoma and D5123 models (0.165 μM and 0.267 μM, respectively). Pathway modulation is linked to efficacy using an integrated PK/PD model revealing a steep relationship where > 50% of the activity of GDC-0449 is associated with >80% repression of the Hh pathway. [4]

お薦めの試験操作(参考用のみ)

細胞試験:[2]
  • 細胞株:MDCKII cells
  • 濃度: 20 μM
  • 反応時間:2 hours
  • 実験の流れ:MDCKII cells are seeded into 24-well plates at a density of 3 × 105 cells per well and are allowed to attach. Medium is then changed to that containing different drugs (50 μM VP, 50 μM indomethacin, or 20 μM GDC-0449 in DMSO or DMSO alone as control, and nonfluorescent calcein-AM is added to a final concentration of 1.0 μM and incubated at 37 °C for 2 hours. Cells are then washed twice with Ca2+, Mg2+-containing Hank's balanced salt solution buffer and lysed by shaking in 0.01% Triton X-100 in PBS buffer for 1 hour at room temperature or overnight at 4 °C. The lysate is then transferred into 96-well plates, and the fluorescence signal caused by the cell-derived calcein is quantified spectrophotometrically with a SpectraMax M5 Multi-Detection Readerusing an excitation wavelength of 495 nm and an emission wavelength of 515 nm. All manipulations are performed in the dark. All readings are expressed as mean ?SEM normalized to the control.
動物試験:[4]
  • 動物モデル:Ptch(+/-) allograft model, D5123 and 1040830
  • 投薬量:~ 100 mg/kg
  • 投与方法:Orally

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
混合させたのち直ちに使用することを推奨します。

12.5mg/mL

化学情報

分子量 421.3
化学式

C19H14Cl2N2O3S

CAS No. 879085-55-9
Storage 3年 -20°C
2年 -80°C in solvent
Smiles CS(=O)(=O)C1=CC(=C(C=C1)C(=O)NC2=CC(=C(C=C2)Cl)C3=CC=CC=N3)Cl

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03610022 Recruiting Drug: Treatment with vismodegib Metastatic Basal Cell Carcinoma|Locally Advanced Basal Cell Carcinoma University Hospital Bordeaux September 3 2018 Phase 4
NCT03035188 Completed Drug: Vismodegib Basal Cell Carcinoma SRH Wald-Klinikum Gera GmbH January 2017 Phase 2
NCT02781389 Completed -- Basal Cell Carcinoma University Hospital Essen|OnkoDataMed GmbH April 29 2016 --
NCT02593760 Completed Other: Placebo|Drug: Ruxolitinib|Drug: Vismodegib Myelofibrosis Hoffmann-La Roche January 25 2016 Phase 1
NCT02648048 Completed Drug: Pirfenidone|Drug: Vismodegib Idiopathic Pulmonary Fibrosis Hoffmann-La Roche January 15 2016 Phase 1
NCT02436408 Completed Drug: Vismodegib Carcinoma Basal Cell University of Michigan Rogel Cancer Center July 15 2015 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

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