A-769662

For research use only. Not for use in humans.

製品コードS2697

A-769662化学構造

CAS No. 844499-71-4

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

サイズ 価格(税別)  
JPY 26400
JPY 80000
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製品安全説明書

AMPK阻害剤の選択性比較

生物活性

製品説明 A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.
ターゲット
AMPK [1]
(Cell-free assay)
Fatty acid synthesis [1]
(in primary rat hepatocytes)
0.8 μM(EC50) 3.2 μM
体外試験

A-769662 stimulates partially purified rat liver AMPK with EC50 with 0.8 μM. A-769662 activates AMPK purified from multiple tissues and species in a dose-responsive manner with modest variations in observed EC50s. EC50s determined for A-769662 using partially purified AMPK extracts from rat heart, rat muscle, or human embryonic kidney cells (HEKs) are 2.2 mM, 1.9 mM, or 1.1 mM, respectively. A 4 hours treatment of primary rat hepatocytes with A-769662 dose-dependently increases ACC phosphorylation, which correlated inhibition of fatty acid synthesis with IC50 of 3.2 μM. A-769662 also inhibits fatty acid sythesis in mouse hepatocytes with IC50 with 3.6 μM [1] A-769662 activates AMPK both allosterically and by inhibiting dephosphorylation of AMPK on Thr-172, similar to the effects of AMP. [2] A-769662 inhibits proteasomal function by an AMPK-independent mechanism. A-769662 affects the in vitro activity of purified 26S proteasomes but not the in vitro activity of purified 20S proteasomes. A-769662 has toxic effects on MEF cells. [3] A recent research shows A-769662 inhibited cell proliferation and DNA synthesis. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse hepatocytes Mo\wSpVv[3Srb36gZZN{[Xl? NFjRUXgyKG2P MoXoSG1UVw>? NEP5TYVqdmirYnn0d{Bn[XS2eTDhZ4llKHO7boTo[ZNqeyC5aYToJGlEPTBib3[gN{43KM7:TR?= NFrN[HY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNke1N|U4Pid-MU[3OVM2PzZ:L3G+
rat hepatocytes MXPGeY5kfGmxbjDhd5NigQ>? NFzmT|kyKG2P MXTEUXNQ MXHpcohq[mm2czDmZZR1gSCjY3nkJJN6dnSqZYPpd{B4cXSqIFnDOVAhd2ZiMz62JO69VQ>? NWTVcnltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3OVM2PzZpPkG2O|U{PTd4PD;hQi=>
HEK293 MljpT4lv[XOnIHHzd4F6 MXGyNFAh|ryP NETHXpZFVVOR NEDxcoli[3SrdnH0[ZMh\W6mb3flco92eyCDTWDL NIDXXVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{eyPFI1OSd-MUe3NlgzPDF:L3G+
CCL13 M1fzXGtqdmG|ZTDhd5NigQ>? NGGxNpozODBizszN NEnwTFZFVVOR NFPESnNi[3SrdnH0[ZMh\W6mb3flco92eyCDTWDL NV3oUmpHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlgzPDFpPkG3O|I5OjRzPD;hQi=>
MEFs NHHLN3JHfW6ldHnvckBie3OjeR?= Mmm3N|AxKM7:TR?= MlLjSG1UVw>? Ml61bY5pcWKrdIOgdJJwfGWjc3;tZYwh\nWwY4Tpc44h[nliYX6gRW1RUy2rbnTldIVv\GWwdDDt[YNp[W6rc32= M4XFfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUmzOVg1Lz5zOEW5N|U5PDxxYU6=
epididymal clear cells NX3Bb3RNTnWwY4Tpc44h[XO|YYm= MnWxNlAxKM7:TR?= NWjJbVhbTE2VTx?= MlHjbY5pcWKrdIOgeIhmKHCKLX3l[IlifGWmIG[tRXRR[XOnIHHjZ5VufWyjdHnvckBifCC2aHWgZZBq[2GuIH3lcYJz[W6n NEC2WWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKxNVkyQCd-MUmyNVE6OTh:L3G+
3T3-L1 MUXGeY5kfGmxbjDhd5NigQ>? NWrqfIEyOS5{IH3N M2jrRWROW09? NVTRZnpUcW6qaXLpeJMhO1R|LVyxJGFlcXCxZ3Xu[ZNqew>? NF3tbIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4N|MxPCd-MUm0PFM{ODR:L3G+
3T3-L1 MXTGeY5kfGmxbjDhd5NigQ>? Mm\3NU4zKG2P M3nUe2ROW09? M1LvTolvcGmkaYTzJJRp\SCHeIDy[ZN{cW:wIH;mJGFlcXCxZ3Xu[ZNqe1KnbHH0[YQhXHKjboPjdolxfGmxbjDGZYN1d3K|IHHu[EBO[XKtZYLz MojhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2OEOzNFQoRjF7NEizN|A1RC:jPh?=
3T3-L1 MoS2SpVv[3Srb36gZZN{[Xl? MlTmNU4zKG2P MXHEUXNQ NIH3dphqdmirYnn0d{BOcXSxdHnjJGNtd26jbDDFfJBidnOrb36= NWL4XYFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0PFM{ODRpPkG5OFg{OzB2PD;hQi=>
3T3-L1 MmXqZ5l1d3SxeHnjbZR6KGG|c3H5 NVXyWFVnOS5{IH3N MX\EUXNQ MkD4[IVkemWjc3XzJGNmdGxiVnnhZoltcXS7 NE\oZXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4N|MxPCd-MUm0PFM{ODR:L3G+
3T3-L1 NYTUNFFnU2mwYYPlJIF{e2G7 NFXiSnYyNjJibV2= NWjDfFg3TE2VTx?= NUTZTXBF[WO2aY\heIV{KEGPUFu= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
L6 skeletal muscle cells NHS1PWVHfW6ldHnvckBie3OjeR?= M2LTTVI2OCEQvF2= NVnwVVAzTE2VTx?= NXHhbHJw[WO2aY\heIV{KEGPUFugd4lodmGuaX7nJJBifGi5YYnz NUnVeZBsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlg5OzZpPkG5PFI5QDN4PD;hQi=>
L6 skeletal muscle cells MVfGeY5kfGmxbjDhd5NigQ>? Mn\nNlUxKM7:TR?= MYTEUXNQ NW\ISG5PcW6qaXLpeJMhfGinIF7hL{1MMy2DVGDhd4UhfHKjboPwc5J1KGGldHn2bZR6KGGwZDDj[YxtKHO3cn\hZ4Uh[WK3bnThcoNm MoH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mki4N|YoRjF7OEK4PFM3RC:jPh?=
MDA-MB231 NUS4OZk{SXCxcITvd4l{KGG|c3H5 MmfrOFAxKM7:TR?= NITmd45FVVOR M13YOZNmdnOrdHn6[ZMhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmW|IITvJHRTSUmOLXnu[JVk\WRiYYDvdJRwe2m| NXHLepdQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PVY1PjlpPkG5PFk3PDZ7PD;hQi=>
BT474 MnHlRZBweHSxc3nzJIF{e2G7 M4XMWlQxOCEQvF2= NE\BW|JFVVOR M3TiVJNmdnOrdHn6[ZMhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmW|IITvJHRTSUmOLXnu[JVk\WRiYYDvdJRwe2m| NUi5[WNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PVY1PjlpPkG5PFk3PDZ7PD;hQi=>
MCF7 MXLBdI9xfG:|aYOgZZN{[Xl? M2jpeVQxOCEQvF2= NYq0XIhuTE2VTx?= NG\NRpZ{\W6|aYTpfoV{KGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscEBtcW6nczD0c{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orcx?= NY\XeG9URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PVY1PjlpPkG5PFk3PDZ7PD;hQi=>
Mesenchymal stem cells NVvsVphJU2mwYYPlJIF{e2G7 M17zWlExKML3TR?= M4TVb2ROW09? NF75[WlqdmS3Y3XzJIEhem:kdYP0JIFv\CC|dYP0ZYlv\WRiQV3QT{Bi[3SrdnH0bY9v NX;aPYZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNFQ5PzlpPkK0NVA1QDd7PD;hQi=>
Mesenchymal stem cells MWXjfZRwfG:6aXPpeJkh[XO|YYm= MnfYNVAxKML3TR?= NXvPXFJHTE2VTx?= NYTqb4pQ\GWlcnXhd4V{KHSqZTDNV2MheHKxbHnm[ZJifGmxbh?= MmfKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMES4O|koRjJ2MUC0PFc6RC:jPh?=
MG-63 NWTlN5Fv[3m2b4TvfIlkcXS7IHHzd4F6 NF3Z[GsyOCEEtV2= MVjEUXNQ MknWbY5pcWKrdIOgTFJQOi2LbnT1Z4VlKE:|dHXvZoxie3RiQ3XscEBF\WG2aB?= NXTMfnZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA{PjJpPkK0PVYxOzZ{PD;hQi=>
MC3T3-E1 MnHvZ5l1d3SxeHnjbZR6KGG|c3H5 MVWxNEDDvU1? NYn4[Y9bTE2VTx?= NEXyRm1qdmirYnn0d{BJOk9{LVnu[JVk\WRiT4P0[Y9jdGG|dDDD[YxtKESnYYTo NEDYXm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFM3Oid-MkS5OlA{PjJ:L3G+
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MC3T3-E1 Mkm0RZBweHSxc3nzJIF{e2G7 M2HUR|ExKML3TR?= NEPVdpFFVVOR M1HtWpN2eHC{ZYPz[ZMhUDKRMj3JcoR2[2WmIF;zeIVw[myjc4SgR4VtdCCDcH;weI9{cXN? NECwVYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFM3Oid-MkS5OlA{PjJ:L3G+
MG-63 Mn71SpVv[3Srb36gZZN{[Xl? MoXCNVAhyrWP NIPNRZBFVVOR M4nHWIFtdGW4aXH0[ZMhWk:VIHHjZ5VufWyjdHnvckBidmRiQWTQJIRmeGyndHnvckBk[XW|ZXSgZpkhUDKRMh?= MmnmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN|YzRC:jPh?=
MC3T3-E1 NXLFcIVZTnWwY4Tpc44h[XO|YYm= MVWxNEDDvU1? NUWzNmpITE2VTx?= M3zyXIFtdGW4aXH0[ZMhWk:VIHHjZ5VufWyjdHnvckBidmRiQWTQJIRmeGyndHnvckBk[XW|ZXSgZpkhUDKRMh?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4MEO2Nkc,OjR7NkCzOlI9N2F-
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PC3M NF7MRoZMcW6jc3WgZZN{[Xl? M4Xr[VExOCEEtV2= MnrWSG1UVw>? MVf1dJJm\3WuYYTld{B1cGVibHX2[Yx{KG:oIFHNVGsh[W6mIFHDR{BxcG:|cHjvdplt[XSrb36= MnPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUSwOFMoRjJ3NUm0NFQ{RC:jPh?=
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PC3 MnzmS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? MnTuNVAxKML3TR?= MXPEUXNQ Mnjod5VxeHKnc4Pld{Bxem:uaX\ldoF1cW:w NVT4W|hlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVQxPDNpPkK1OVk1ODR|PD;hQi=>
PC3M NFnEZnZIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MojLNVAxKML3TR?= NECwTIhFVVOR NH\VT29{fXCycnXzd4V{KHC{b3zp[oVz[XSrb36= M3exS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUm0NFQ{Lz5{NUW5OFA1OzxxYU6=
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NCI-H460 MmjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonqTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSES2NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hPDhwMUK1OUDPxE1w NGfVSJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTJ3MESvK|5USU6JRWK8M4E,
D-336MG NFHodFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvrRmdvUW6qaXLpeIlwdiCxZjDoeY1idiCGLUOzOm1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSB2OD60PFQ2KM7:TT6= MkfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
SNG-M NFracWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIixTXhKdmirYnn0bY9vKG:oIHj1cYFvKFOQRz3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA1QC54N{CxJO69VS5? NVjH[m94RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
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CAL-27 NWjIXFR4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\vOWlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA1QS53M{O0JO69VS5? MkPpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
8505C MnjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vIXGlvcGmkaYTpc44hd2ZiaIXtZY4hQDVyNVOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ6NjV|NkSg{txONg>? NGD0dpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTJ3MESvK|5USU6JRWK8M4E,
MCF7 NIDCfXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInDOFFKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ6NjV6NkWg{txONg>? M3PK[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
T47D NXv2O3ltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGi5XmhKdmirYnn0bY9vKG:oIHj1cYFvKFR2N1SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ6NjdyMEeg{txONg>? Mk[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
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他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western blot
pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin ; 

PubMed: 26431377     


A & B. MCF-7 cells were treated with 100–400 μM of A-769662 for 4-24 hours. Protein was harvested and Western blot analysis was done to determine levels of pAMPK at threonine 172, pCofilin at serine 3, detyrosinated alpha tubulin (glu-tub), and acetylated alpha tubulin (acetyl-tub). C & D. BT-549 cells were treated with 100–400 μM A-769662 for 4–24 hours. Protein was harvested and Western blot analysis was done to determine levels of markers listed above.

p-AMPK / AMPK / p-S6K / S6K / p-ACC ; 

PubMed: 22456226     


Representative immunoblots for p-AMPK, total AMPK, p-S6K, total S6K, p-ACC and actin under control conditions (C) and in response to 50 μM A-769662 at various time points.

26431377 22456226
体内試験 Short-term treatment of normal Sprague Dawley rats with A-769662 decreases liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreases hepatic expression of PEPCK, G6Pase, and FAS, lowers plasma glucose by 40%, reduced body weight gain and significantly decreases both plasma and liver triglyceride levels. [1]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[1]

- 合併

96-well AMPK assay:

AMPK activity is measured by monitoring phosphorylation of the SAMS peptide substrate (20 mM in standard assays and 100 mM in additivity assays) following a previously described protocol (Anderson et al., 2004). To determine whether A-769662-induced AMPK activation occurs in a reversible manner, AMP or A-769662 are preincubated with rat liver AMPK for 10 minutes at 20 times standard assay concentrations prior to dilution and measurement of AMPK activity.
細胞試験:

[3]

- 合併
  • 細胞株: MEF cells
  • 濃度: 300 μM
  • 反応時間: 24 hours
  • 実験の流れ:

    Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective.


    (参考用のみ)
動物試験:

[1]

- 合併
  • 動物モデル: Sprague Dawley rats
  • 投薬量: 30 mg/kg
  • 投与方法: Administered via i.p.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 72 mg/mL (199.78 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
混合させたのち直ちに使用することを推奨します。
5 mg/ml

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 360.39
化学式

C20H12N2O3S

CAS No. 844499-71-4
Storage powder
in solvent
別名 N/A
Smiles C1=CC=C(C(=C1)C2=CC=C(C=C2)C3=CSC4=C3C(=C(C(=O)N4)C#N)O)O

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

AMPKシグナル伝達経路

相関AMPK製品

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID