Deferoxamine mesylate

別名:Ba 33112, Desferrioxamine B, DFOM, NSC 644468, DFO

Deferoxamine mesylate is the mesylate salt of Deferoxamine, which forms iron complexes and is used as a chelating agent. Deferoxamine is a ferroptosis inhibitor that stabilizes HIF-1α expression and improves HIF-1α transactivity in hypoxic and hyperglycemic states in vitro. Deferoxamine decreases beta-amyloid (Aβ) deposition and induces autophagy.Please do not prepare stock solutions with normal saline or PBS, as precipitation may occur.

Deferoxamine mesylate化学構造

CAS No. 138-14-7

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 29500 国内在庫あり
JPY 22000 国内在庫あり
JPY 59500 国内在庫あり
JPY 295500 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(73)

製品安全説明書

現在のバッチを見る: 純度: 99.74%
99.74

Deferoxamine mesylateと併用されることが多い化合物

Z-VAD-FMK


Deferoxamine mesylate can reverse the cell proliferative activity of granulosa cells in EMFF but not Z-VAD-FMK.

Ni Z, et al. Cell Death Dis. 2022 Jul 4;13(7):579.

Fer-1 (Ferrostatin-1)


Deferoxamine mesylate and Ferrostatin-1 can reverse the cell proliferative activity of granulosa cells in EMFF.

Ni Z, et al. Cell Death Dis. 2022 Jul 4;13(7):579.

SRS11-92


Deferoxamine mesylate can reverse the cell proliferative activity of granulosa cells in EMFF but not Necrostatin-1.

Ni Z, et al. Cell Death Dis. 2022 Jul 4;13(7):579.

Rosiglitazone


Deferoxamine mesylate and Rosiglitazone are inhibitors of ferroptosis and induce autophagy.

Deferoxamine mesylate関連製品

Ferroptosis阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
HT-29 Cell cycle assay 10 uM 24 hrs Cell cycle arrest in nocodazole pretreated human HT-29 cells assessed as accumulation at M phase at 10 uM after 24 hrs 20353152
HT-29 Cell cycle assay 5 uM 24 hrs Cell cycle arrest in nocodazole pretreated human HT-29 cells assessed as accumulation at M phase at 5 uM after 24 hrs 20353152
HeLa Bacteriostatic assay >100 uM 1 hr Bacteriostatic activity against Chlamydia trachomatis serovar L2 infected in human HeLa cells assessed as growth inhibition compound treated at >100 uM for 1 hr prior infection measured 24 hrs post infection by microscopy 25027937
SK-N-BE(2)-M17 Cytoprotection assay 1 mM 2 hrs Cytoprotection against PQ-induced cell death in human SK-N-BE(2)-M17 cells assessed as increase in cell viability at 1 mM pretreated for 2 hrs followed by 1 mM PQ addition measured after 24 hrs by LDH assay 28285915
SK-N-BE(2)-M17 Cytoprotection assay 1 mM 2 hrs Cytoprotection against H2O2-induced cell death in human SK-N-BE(2)-M17 cells assessed as increase in cell viability at 1 mM pretreated for 2 hrs followed by 1 mM H2O2 addition measured after 24 hrs by LDH assay 28285915
SK-N-BE(2)-M17 Cytoprotection assay 1 mM 2 hrs Cytoprotection against PQ-induced cell death in human SK-N-BE(2)-M17 cells assessed as increase in cell viability at 1 mM pretreated for 2 hrs followed by 1 mM PQ addition measured after 24 hrs by MTT assay 28285915
SK-N-BE(2)-M17 Cytoprotection assay 1 mM 2 hrs Cytoprotection against H2O2-induced cell death in human SK-N-BE(2)-M17 cells assessed as increase in cell viability at 1 mM pretreated for 2 hrs followed by 1 mM H2O2 addition measured after 24 hrs by MTT assay 28285915
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 4.51 μM. 17602603
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 5 μM. 19601577
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 7.35 μM. 19216562
A549 Antiproliferative assay 72 hrs Antiproliferative activity at human A549 cells after 72 hrs by MTT assay, IC50 = 7.5 μM. 22861499
SK-N-MC Antiproliferative assay 96 hrs Antiproliferative activity against human SK-N-MC cells after 96 hrs by MTT assay, IC50 = 8.58 μM. 17064069
SK-N-MC Cytotoxicity assay 72 hrs Cytotoxicity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 9.89 μM. 20303768
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity at human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 10 μM. 22861499
DMS53 Antiproliferative assay 72 hrs Antiproliferative activity at human DMS53 cells after 72 hrs by MTT assay, IC50 = 10 μM. 22861499
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 12.5 μM. 22858101
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 16.81 μM. 28841514
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 17.07 μM. 21055950
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay, IC50 = 17.07 μM. 22172311
U2OS Function assay 30 mins Activation of human HIF1alpha expressed in DFX-induced human U2OS cells incubated for 30 mins prior to DFX-induction measured after overnight incubation by luciferase reporter gene assay, EC50 = 17.8 μM. 22172704
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells measured after 72 hrs at 37 degC by MTT assay, IC50 = 21.63 μM. 23312948
SK-N-MC Antiproliferative assay 72 hrs Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTS assay, IC50 = 22.7 μM. 21846118
SK-N-MC Cytotoxicity assay 72 hrs Cytotoxicity against human SK-N-MC cells assessed as growth inhibition after 72 hrs by MTT assay, IC50 = 22.7 μM. 23276209
HT29 Cytotoxicity assay 72 hrs Cytotoxicity against human HT29 cells after 72 hrs by MTS assay, IC50 = 36.1 μM. 18345610
HCT116 Function assay 24 hrs Photocytotoxicity against human HCT116 cells expressing wild type p53 incubated for 24 hrs followed by light irradiation measured after 24 hrs by MTS assay in presence of ALA and FeCl3 24900837
MDCK Toxicity assay Toxicity in MDCK cells by MTT method, IC50 = 9.49 μM. 20041672
SK-MN-C Antiproliferative assay Antiproliferative activity against human SK-MN-C cells by MTT assay, IC50 = 12.05 μM. 18159922
SK-N-MC Antiproliferative assay Antiproliferative activity against human SK-N-MC cells by MTT assay, IC50 = 14.22 μM. 17963372
SK-N-MC Toxicity assay Toxicity in human SK-N-MC cells by MTT method, IC50 = 16.04 μM. 20041672
HL60 Cytotoxicity assay Cytotoxicity against human HL60 cells by alamar blue assay, GI50 = 18.4 μM. 23266185
SK-N-MC Antiproliferative assay Antiproliferative activity against human SK-N-MC cells, IC50 = 22 μM. 19601577
K562 Cytotoxicity assay Cytotoxicity against human K562 cells, GI50 = 33.1 μM. 23266185
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
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生物活性

製品説明 Deferoxamine mesylate is the mesylate salt of Deferoxamine, which forms iron complexes and is used as a chelating agent. Deferoxamine is a ferroptosis inhibitor that stabilizes HIF-1α expression and improves HIF-1α transactivity in hypoxic and hyperglycemic states in vitro. Deferoxamine decreases beta-amyloid (Aβ) deposition and induces autophagy.Please do not prepare stock solutions with normal saline or PBS, as precipitation may occur.
Targets
HIF-1α [1] Beta Amyloid [1] Ferroptosis [2]
In Vitro
In vitro

Deferoxamine mesylate, the iron chelator and ferroptosis inhibitor, rescues neutrophil death induced by systemic lupus erythematosus (SLE) serum, suggesting that ferroptosis may be the main form of neutrophil death in SLE.[3]

細胞実験 細胞株 Healthy neutrophil cell
濃度 1/10/100 μM
反応時間 16 h
実験の流れ

Cells are cultured in complete RPMI 1640 basic medium (Gibco) with 100 U/ml penicillin, 100 μg/ml streptomycin and 20% serum from ten randomly selected patients with SLE or age- and sex-matched healthy controls (HCs), and incubated at 37 ℃ in a humidified atmosphere of 20% O2 and 5% CO2. HC neutrophil cell viability treated with two ferroptosis inhibitors, LPX-1 (10/100/1,000 nM) or DFO (1/10/100 μM), two necroptosis inhibitors, Nec-1 (10/100/1,000 nM) or NSA (10/100/1,000 nM), or apoptosis inhibitor Z-VAD (0.1/1/10 μM) for 16 h are assessed by flow cytometry.

In Vivo
In Vivo

Deferoxamine mesylate (Ba 33112, Desferrioxamine B, DFOM, NSC 644468) is the mesylate salt of Deferoxamine, which forms iron complexes and is used as a chelating agent.

動物実験 動物モデル Mongrel dogs
投与量 25 mg/kg
投与経路 i.m.

化学情報

分子量 656.79 化学式

C26H52N6O11S

CAS No. 138-14-7 SDF --
保管 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (152.25 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 100 mg/mL

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Handling Instructions

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