Apigenin

For research use only. Not for use in humans.

製品コードS2262 別名:NSC 83244, LY 080400

Apigenin化学構造

分子量(MW):270.24

Apigenin is a potent P450 inhibitor for CYP2C9 with Ki of 2 μM.

サイズ 価格(税別) 在庫  
10mM (1mL in DMSO) JPY 16900 あり
JPY 13600 あり
JPY 21900 あり
JPY 36800 あり
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バルク問合せ

カスタマーフィードバック(4)

  • Western blotting confirmed that both GLUT-1 (A) and p-Akt (B) were expressed in Hep-2 cells in different apigenin and cisplatin concentration.

    Int J Clin Exp Pathol, 2014, 7(7):3938-3.. Apigenin purchased from Selleck.

    SKH-1 mice were subjected to UVB radiation (1000 J/m2 daily, 5 days), or topical apigenin (Api, 5 μmol, in 200 μl vehicle, DMSO: acetone 1:9) was applied 1 h prior to each UVB exposure. Mice were also sham-irradiated and treated with apigenin, and the control group of mice was subjected to sham irradiation and vehicle. 24 h after the final UVB exposure, the mice were euthanized, dorsal skin was harvested, fixed in formalin and paraffin-embedded. (A) Representative Hematoxylin and Eosin (H&E) staining of skin sections (scale bar, 100 μm). (B) Quantitation of epidermal thickness (mean ± SD), *, P < 0.001; **, P< 0.0001. Three sections per mouse and 3 mice per group were evaluated.

    Cell Signal, 2016, 28(5):460-468. Apigenin purchased from Selleck.

  • The effects of apigenin and GLUT-1 AS-ODNs on xenograft apoptosis (the second experiment), observed an under optical microscope (magnification, ×400), and the percentage of apoptotic cells in 100 cells per field was counted and used to calculate the mean apoptosis index (AI). AI, apoptotic index.

    Oncol Rep, 2015, 34(4):1805-14. Apigenin purchased from Selleck.

    GLUT-1 mRNA expression levels were significantly reduced in the ACC-2 human adenoid cystic carcinoma cells following treatment with increasing doses of apigenin (P<0.05), as determined by reverse transcription-quantitative polymerase chain reaction. Following treatment with 10 µM apigenin, the expression levels of GLUT-1 mRNA did not vary significantly with increasing treatment duration (P>0.05). Following treatment with 40 and 160 µM apigenin, the expression levels of GLUT-1 mRNA were significantly reduced with increasing treatment duration. *P<0.05 vs. control. GLUT-1, glucose transporter-1.

    Mol Med Rep, 2015, 12(5):6461-6. Apigenin purchased from Selleck.

製品安全説明書

P450 (e.g. CYP17)阻害剤の選択性比較

生物活性

製品説明 Apigenin is a potent P450 inhibitor for CYP2C9 with Ki of 2 μM.
特性 Much more potent than kaempferol and myricetin in CT-L inhibition.
ターゲット
CYP2C9 [5]
2 μM(Ki)
体外試験

Apigenin inhibits PKC by competing with adenosine triphosphate (ATP). Apigenin also reduces the level of TPA-stimulated phosphorylation of cellular proteins and inhibits TPA-induced c-jun and c-fos expression. Apigenin exhibits the reverting effect on the transformed morphology of v-H-ras transformed NIH3T3 cells. [1] Apigenin has been shown to possess anti-mutagenic properties in a setting of nitropyrene-induced genotoxicity in Chinese hamster ovary cells. Apigenin suppresses of LPS-induced cyclooxygenase-2 and nitric oxide synthase-2 activity and expression in mouse macrophages. Apigenin has been reported to inhibit protein kinase C activity, mitogen-activated protein kinase (MAPK), transformation of C3HI mouse embryonic fibroblasts and downstream oncogenes in v-Ha-ras-transformed NIH3T3 cells. Apigenin blocks peroxisome proliferation-regulated kinase (ERK), a MAPK in isolated hepatocytes. Apigenin has further been shown to down-regulate the expression of the Na+/Ca2+-exchanger, a protein important for calcium extrusion in neonatal rat cardiac myocytes. Apigenin induces a reversible G2/M and G0/G1 arrest by inhibiting p34 (cdc2) kinase activity, accompanied by increased p53 protein stability in epidermal cells and fibroblasts. Apigenin is also effective in inhibiting TNFα-induced intracellular adhesion molecule-1 upregulation in cultured human endothelial cells. Apigenin inhibits the expression of HIF-1α and VEGF via the PI3K/Akt/p70S6K1 and HDM2/p53 pathways in human ovarian cancer cells. [2] Apigenin inhibits differentiation by suppressing MAPK signal transduction and reducing API transcription factor level in human keratinocytes. Apigenin also inhibits proliferating of human keratinocytes. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells MV3GeY5kfGmxbjDhd5NigQ>? MVrJcohq[mm2aX;uJI9nKEORWEKgdJJwfGWrbjDlfJBz\XO|aX;uJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|LDDJR|UxRTBwNTFOwG0> MmrvNVYxOzh3M{[=
human H295R cells M4O5RWZ2dmO2aX;uJIF{e2G7 NXz3O|lSUW6qaXLpeIlwdiCxZjDhdo9u[XSjc3Wg[ZhxemW|c3XkJIlvKGi3bXHuJGgzQTWUIHPlcIx{NCCLQ{WwQVEh|ryP NVr6[3VXOTh5N{i5OFQ>
HEK293 FS cells NEC1S45HfW6ldHnvckBie3OjeR?= MXzJcohq[mm2aX;uJI9nKE6RWESg[ZhxemW|c3XkJIlvKEiHS{K5N{BHWyClZXzsd{Bie3Onc4Pl[EBieyCKMl:yJJBzd2S3Y4Tpc44h[nliSELPNk9VgXJxTGDPJIF{e2G7LDDJR|UxRTFwMUOg{txO NFvVR3ozODd|MUO1Oy=>
human HeLa cells NGLMPZBHfW6ldHnvckBie3OjeR?= MnXvTY5pcWKrdHnvckBw\iCPUmCxJJRz[W6|ZnXjeIVlKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCdM1jdUHREPCC2cnHud5BwenRiYomgdoFxcWRiZnnseJJifGmxbjDhd5NigSxiS3m9Nk41KM7:TR?= NF;2WWEyQTd{NUW3PC=>
human MV4-11 cells NI\wR2xEgXSxdH;4bYNqfHliYYPzZZk> NVvzVIhYPzJiaB?= M4O1UGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1XPC1zMTDj[YxteyCqYYLic5JqdmdiRlzUN{BufXSjdHnvckBi\nSncjC3NkBpenNiYomgeIV1emG8b3zpeY0h[mG|ZXSgSZohS3mWb4igZ4VtdCC4aXHibYxqfHliYYPzZZktKEeLNUC9Nk45OSEQvF2= MVqyN|QyOTB5Mx?=
human mast cells M{DNfGZ2dmO2aX;uJIF{e2G7 NXvzNHFiUW6qaXLpeIlwdiCxZjDTXWshcW5iaIXtZY4hdWG|dDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hdWG|dDDj[YxtKGSnZ4LhcpVt[XSrb36sJGVEPTB;MzFOwG0> MUWyNVM2PDhyMB?=
MDCK cells MkjNSpVv[3Srb36gZZN{[Xl? M{nuTmlvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhcW5iTVTDT{Bk\WyuczD1d4lv\yCKb3XjbJN1KDN|M{SyJJN1[WmwaX7nMEBKSzVyPUOuNUDPxE1? MU[yNVM2PDhyMB?=
human MDA-kb2 cells NFfuemJHfW6ldHnvckBie3OjeR?= MoDtRY51[WexbnnzeEBi[3Srdnn0fUBifCCjbnTyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCPRFGtb4IzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gSGhVNWmwZIXj[YQhdHWlaX\ldoF{\SCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9OU4zKM7:TR?= Ml3sNVk2QTJ{NEW=
MCF-7 MX cells NHrNZW9HfW6ldHnvckBie3OjeR?= MWLJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJIlvKE2FRj23JG1ZKGOnbHzzJJV{cW6pIFjv[YNpe3RiM{OzOFIhe3SjaX7pcoctKEmFNUC9OU46KM7:TR?= MX2yNVM2PDhyMB?=
mouse RAW264.7 cells M4\hcGZ2dmO2aX;uJIF{e2G7 MXiyOEBp NG\JepFCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCwaYTybZRmKGGlY4XteYxifGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQhdWW2aH;kMEBKSzVyPU[uO{DPxE1? Mk[wNVk4PzhyOE[=
human H9 cells MorTSpVv[3Srb36gZZN{[Xl? M2rRRVMh\GG7cx?= NHzGOIFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IICyOEBidnSrZ3XuJINieHS3cnWgZZN{[XluIFXDOVA:QSEQvF2= MUG4NVU5OTZ2
HEK293 cells NGW3cWRHfW6ldHnvckBie3OjeR?= NVnBRlB7OjRiaB?= M3HmW2Fod26rc4SgZYN1cX[rdImgZZQhdW:3c3WgVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ48u\XiycnXzd4lv\yC5aYToJGdidDRicnXwc5J1\XJidnXjeI9zKGGodHXyJFI1KGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFXDOVA:OjRwOTFOwG0> MkPSNlQ6PTV6OEm=
mouse 26-L5 cells NWjvOFRJWHKxbHnm[ZJifGmxbjDhd5NigQ>? NUDp[oxyPzJiaB?= Ml3qRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SB{Nj3MOUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTJ3IN88US=> NUCzSWgzOTJyMke3N|k>
human RS4:11 cells NH:5SYpEgXSxdH;4bYNqfHliYYPzZZk> NILISlE4OiCq MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSV|Q7OTFiY3XscJMhcGG{Yn;ybY5oKHerbHSgeJlx\SCITGSzJIFnfGW{IEeyJIhzeyCkeTD0[ZRz[XqxbHn1cUBj[XOnZDDFfkBEgVSxeDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhT0l3ME2yO{46KM7:TR?= NF\ORoIzOzRzMUC3Ny=>
mouse B16-BL6 cells NUPt[HM{WHKxbHnm[ZJifGmxbjDhd5NigQ>? NHi0S3Y4OiCq MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEJzNj3CUFYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD1|MT62JO69VQ>? MmmxNVIxOjd5M{m=
human H9 cells NYfwZpQ3S3m2b4TvfIlkcXS7IHHzd4F6 MWOzJIRigXN? NHHUOoVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJQSClZXzsd{Bi\nSncjCzJIRigXNuIFnDOVA:OzVizszN NXjISWtXQDF3OEG2OC=>
human HT1080 cells MoHpVJJwdGmoZYLheIlwdiCjc4PhfS=> NX;kT|ZvPzJiaB?= NVrxPGQ6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWFExQDBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN? MXuxNlAzPzd|OR?=
MDCK cells M1TmbWN6fG:2b4jpZ4l1gSCjc4PhfS=> NXfYeGF1S3m2b4TvfIlkcXS7IHHnZYlve3RiTVTDT{Bk\WyuczDifUBOXFRiYYPzZZktKEOFNUC9N|kvPTlizszN M4n4eVE5PjRyMESy
mouse L929 cells Mn7USpVv[3Srb36gZZN{[Xl? NWnmRoh3OTVibXnudy=> NXPvSWpyWG:2ZX70bYF1cW:wIH;mJJJm[2:vYnnuZY51KGi3bXHuJHRPTi2jbIDoZU1qdmS3Y3XkJIN6fG:2b4jpZ4l1gSCxZjDtc5V{\SCOOUK5JINmdGy|IHHzd4V{e2WmIHHzJJN2en[rdnHsbZR6KHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bj\W[xcnWgWG5H[WyyaHGgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IHPyfZN1[WxidnnvcIV1KHO2YXnubY5o M3\FdlkzQDd2MUW=
human THP1 cells NILvR3NHfW6ldHnvckBie3OjeR?= MmPWNlAhfU1? M4LyZVEhcA>? NFm3dWVKdmirYnn0bY9vKG:oIF7PXFIhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDkc5dvemWpdXzheIlwdiCxZjDUVGEucW6mdXPl[EBETDN4IH3SUmEh\XiycnXzd4lwdiCjdDCyNEB2VSCrbnP1ZoF1\WRiZn;yJFEhcHJicILpc5IhfG9iVGDBJINp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| NIiyeHkzOzd6NkWyNC=>
MDA-MB-231 cells M4TQV2Z2dmO2aX;uJIF{e2G7 M17WZVUhfU1? M4DubGlvcGmkaYTpc44hd2ZiUF3BMZN1cW23bHH0[YQhVkZva3HwdIFDKHOrZ37hcIlv\yBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDNSGEuVUJvMkOxJINmdGy|IHH0JFUhfU1iaX7jeYJifGWmIH\vdkAyPiCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7 Ml;MNlUyQTB2Nk[=

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western blot
MHC / MHC2A / MHC2B / MyoD ; 

PubMed: 29108230     


Apigenin increases total MHC, MHC2A, MHC2B and myoD expression in C2C12 cells. 

CXCR4; 

PubMed: 23743303     


(C) Apigenin suppressed CXCR4 levels in a dose-dependent manner. Cells were treated with the indicated concentrations of apigenin for 24 hours. CXCR4 expressions were indicated by anti-CXCR4 and anti-GAPDH western blotting analyses. (D) Apigenin suppressed CXCR4 levels in a time-dependent manner. Cells were treated with 40 μM apigenin for the indicated times, after which western blotting was performed as described above.

CDK1 / Cyclin B1 / p21 ; 

PubMed: 24009741     


Immunoblot analysis of G2/M phase regulators in cells treated with apigenin for 24 hr. CT: control.

29108230 23743303 24009741
Immunofluorescence
E-caherin / Vimentin ; 

PubMed: 27203387     


Typical images of immunofluorescent double staining for E-cadherin and Vimentin in Bel-7402 and PLC/PRF/5 cells. Each experiment was performed in triplicate.

Snail; 

PubMed: 27203387     


Typical immunofluorescence images of Snail in Bel-7402 and PLC/PRF/5 cells. Each experiment was performed in triplicate.

27203387
Growth inhibition assay
Cell viability; 

PubMed: 23224239     


Effect of apigenin on cell viability. Cells were treated with various concentrations of apigenin (2–30 μg/ml) for 24 h and cell viability was measured by MTT assay. Statistical significance: *p < 0.001.

23224239
体内試験 Apigenin down-regulates production of IL-4 in ovalbumin-immunized BALB/C mice. Apigenin inhibits melanoma lung metastases by impairing interaction of tumor cells with endothelium. Apigenin is shown to cause a significant increase in uterine weight and overall uterine concentration of estrogen receptor (ER)-α in female mice (64) and also suppresses prostate and breast cancer cell growth through estrogen receptor β1. Apigenin suppresses the levels of IGF-I in prostate tumor xenografts and increases levels of IGFBP-3, a binding protein that sequesters IGF-I in vascular circulation. [2] Apigenin (12.5 mg/kg) increases cell proliferation in the dentate gyrus of hippocampus of adult mice. [4]

お薦めの試験操作(参考用のみ)

細胞試験:

[5]

- 合併
  • 細胞株: WI-38, T-24, HT-1376 and PC-3 cells
  • 濃度: 0, 1, 5, 10, 20, 30, 40, and 50 μg/ml
  • 反応時間: 24 h
  • 実験の流れ:

    To measure the effect of apigenin on cell viability, the WI-38, T-24, HT-1376 and PC-3 cells were seeded in 24-well plates (1 × 105 cells/well) for 16-18 h. The cells were then treated with or without various concentrations (0, 1, 5, 10, 20, 30, 40, and 50 μg/ml) of apigenin for 24 h. Each treatment was repeated 3 times. After the exposure period, the medium was removed and followed by washing the cells with PBS. The medium was then changed and incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (5 mg/ml)/well for 4 h. The medium was removed, and formazan was solubilised in isopropanol and measured spectrophotometrically at 563 nm. The percentage of viable cells was estimated by comparing them with the untreated control cells.


    (参考用のみ)
動物試験:

[6]

- 合併
  • 動物モデル: heterozygous C57BL/TGN TRAMP mice
  • 投薬量: 20 and 50 μg/mouse/day
  • 投与方法: p.o.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 270.24
化学式

C15H10O5

CAS No. 520-36-5
保管
in solvent
別名 NSC 83244, LY 080400
Smiles OC1=CC=C(C=C1)C2=CC(=O)C3=C(O2)C=C(O)C=C3O

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(余分な消耗を考慮し動物一匹分の量を用意することをお勧めします。)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロットごとに組成が異なるため、セレックから完全に溶解できる組成をお求めください。)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

P450 (e.g. CYP17)シグナル伝達経路

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID