Y-27632 2HCl

For research use only. Not for use in humans.

製品コードS1049

Y-27632 2HCl化学構造

CAS No. 129830-38-2

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

サイズ 価格(税別)  
10mM (1mL in DMSO) JPY 27800
JPY 13600
JPY 21900
JPY 30200
JPY 96600
最寄りの販売代理店を探す

お探しのディーラーが見当たらない場合は直接こちらのメールアドレスまでお問い合わせください:[email protected]

バルク問合せ

文献中Selleckの製品使用例(622)

製品安全説明書

ROCK阻害剤の選択性比較

生物活性

製品説明 Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
ターゲット
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外試験

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells MmH0SpVv[3Srb36gRZN{[Xl? NGPF[FAyOCEEtV2= MmT0O{Bl MX;S[YR2[2W|IGPHV2Mhe2WwZYPj[Y5k\Q>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThyNEW2NEc,OjV6MES1OlA9N2F-
HT22 NY\Jd2pxS3m2b4TvfIlkKEG|c3H5 NXTFcXg6OTBiwsXN NXvnbIE4OTNiaB?= MmrrVJJwfGWldIOgZYdicW6|dDDncJV1[W2jdHWtbY5lfWOnZDDu[ZVzd26jbDDk[YF1cA>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhzMEizOUc,OjJ6MUC4N|U9N2F-
Swiss3T3 NXLkSYZNS2:ub375MYZwem2rbnegRZN{[Xl? MUOxNEDDvU1? MnPjNVMh\A>? MVHJcoNz\WG|ZYOgdJJwe3SjdHWgZ4VtdCClb3zvcpku\m:{bXnu[{Bi[3Srdnn0fS=> NF;EeoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS2OFkxOid-MkG0OlQ6ODJ:L3G+
TSGH 8301 M4PrSW1q\3KjdHnvckBCe3OjeR?= MlnUNlAhyrWP NH3aZlcyKGh? NEDkR4lKdmO{ZXHz[ZMh[2WubDDtbYdz[XSrb36= MkLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OU[0O|UoRjF7OEm2OFc2RC:jPh?=
Cynomolgus monkey embryonic stem cells MVLDfZRwfG:6aXOgRZN{[Xl? MlLaNlAhyrWP MV:yOEBp MUDQdo9ud3SnczDjfWVUKGOnbHygd5Vzfmm4YXy= NV3We4pERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi5OFA5PTVpPkG4PVQxQDV3PD;hQi=>
PC 12 NFzHSJBHfW6ldHnvckBCe3OjeR?= NH3FO5IyOMLizszN NY[0VlVJOjRiaB?= MULBeJRmdnWjdHXzJINifGWlaH;sZY1qdmViYnnvd5lvfGinc3nz NWPjT5VsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yNVk1OjRpPkG2NlE6PDJ2PD;hQi=>
C2C12 Ml74SpVv[3Srb36gRZN{[Xl? NG\6SYQyOMLizszN MoLsOkBp M4jrb3Bz\X[nboTzJJRp\SC|ZYLpcoUheGixc4Doc5J6dGG2aX;uJI9nKEmUUz2xJIlv\HWlZXSgZpkhcW6|dXzpckBidmRxb4KgWG5HNc7z MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ4N{GyOEc,OTZ{NkexNlQ9N2F-
Pancreatic acinar cells M4nkPWZ2dmO2aX;uJGF{e2G7 M3LTdVExyqEQvF2= MnvJO|AhdWmw M17JfXBwfGWwdHnheIV{KEOFSz3zeIlufWyjdHXkJJBidmO{ZXH0bYMh\W68eX3lJJNm[3KndHnvci=> MoH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ5NEWwPFAoRjF{N{S1NFgxRC:jPh?=
Rat hepatic stellate cells NX\4WnB1TnWwY4Tpc44hSXO|YYm= NYLCd2RyOzBizszN M1i0SFQ5KGh? MU\EbY1qdmm|aHXzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiRYLrNkwh[W6mIHTlZ5Jm[XOnczDu[ZchTE6DIIP5cpRp\XOrcx?= M2O2N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOES1OlY{Lz5zMEi0OVY3OzxxYU6=
LNCaP MlSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvSWVdmOjVizszN MVmxO{Bp M2S3UGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
PC3 M3\QVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnGNlUh|ryP NICz[lMyPyCq MWDEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
PC3 NI\HOXROcWe{YYTpc44hSXO|YYm= NEnRS2ozPSEQvF2= MX[xJIg> NUn4c3E1UW6qaXLpeJMhfGinIFLNSmIuS01iYX7kJJRp\SCHR1[td5RqdXWuYYTl[EBucWe{YYTpc44> NEmwbpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEeyNFQ4OSd-MUC3NlA1PzF:L3G+
PC3 MUnGeY5kfGmxbjDBd5NigQ>? M1\5VVI2KM7:TR?= NGHze4gyKGh? NFr3dZVKdmS3Y3XzJI1wenCqb3zv[4lk[WxiY3jhcodmew>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
Rat Vascular Smooth Muscle Cells NXPHXYdKTnWwY4Tpc44hSXO|YYm= NV\EWY9uOTBizszN NGXTfFMzKGh? MX7Jcohq[mm2czDhcodqd3SnboPpckBKUS2rbnT1Z4VlKGi7cHXyeJJweGi7 MoXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB4NEKzNVcoRjFyNkSyN|E4RC:jPh?=
Stellate Cell NHzSTYFHfW6ldHnvckBCe3OjeR?= NUjRTXIzOjVizszN NI\2T5EyPSCvaX6= M{HJcmlvcGmkaYTzJIZwem2jdHnvckBw\iCILXHjeIlvKHO2cnXzd{BncWKncoOgZY5lKHCqb4PwbI9zgWyjdHnvckBw\iCveX;zbY4hdGmpaISgZ4hicW5? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZyMES5Okc,OTB4MEC0PVY9N2F-
Neonatal rat ventricular myocytes NXPBcHlETnWwY4Tpc44hSXO|YYm= M3LReVExKM7:TR?= MUe0PEBp MWLJcohq[mm2czDFWE0yNWmwZIXj[YQhcW6lcnXhd4V{KGmwIIDyc5RmcW5ic4nueIhme2m|LDDj[YxtKHOrenWgZY5lKG27b3\pZpJqdGyjcjDvdodidmm8YYTpc44> NV7ndlh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCzPFY3OTNpPkGwN|g3PjF|PD;hQi=>
LS174T NITHbmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LnR|ExKM7:TR?= NWT1c2FwOThiZB?= MXHNc4RmemG2ZXz5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
HCT116 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHtU5UyOCEQvF2= M4nZR|E5KGR? NXPKZZZ2W3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NFv3b289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
HCT15 NIHxTndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYSxNEDPxE1? MVGxPEBl NWTu[YI3TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NEfucXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
SW620 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmC0NVAh|ryP MoXXNVgh\A>? NIKyZXhFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NWL0OJd2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Src-2 NG\5NIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\WNG4yOCEQvF2= NXfLcVZWOThiZB?= MnXiSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> M1T2VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Src-1 MkLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVuxNEDPxE1? Mk\CNVgh\A>? MWDEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NHfDcIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
NIH3T3 NW\MOpBQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3v1clExKM7:TR?= MVOxPEBl NI\VVmxFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NFy3XYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-4 NUftfY1OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVqxNEDPxE1? MnGyNVgh\A>? MWjEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NWHCUYlKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Src-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjtNVAh|ryP NYfZZ|BmOThiZB?= MVnEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To M2\ONVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Ras-4 MljDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7XWpMyOCEQvF2= M{P2WVE5KGR? MknHV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NGLLbm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Ras-2 NYT1bWNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLHNVAh|ryP NILk[poyQCCm MnfBV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
mNET1-e NUHGPFQ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETDUngyOCEQvF2= M3zsfFE5KGR? NEHPNoVUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M1\jN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
mNET1-d MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYWxNEDPxE1? NHXJRokyQCCm MYXTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NUPzRmpRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Dbl-e NIr0bWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\VR|ExKM7:TR?= MYKxPEBl M2XsOW1w\GW{YYTlcJkhcW6qaXLpeJMh[2WubDDndo94fGh? M1r6cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Dbl-d MmXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUWxTlBxOTBizszN NVjMSHl4OThiZB?= NUfsWJZNW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NYrUNZppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
NIH3T3 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnJRVVFOTBizszN NYPTZo05OThiZB?= M2HUVWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> M4S5RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Mesothelial cells from rat mesentery M2TMWGlvfmG|aY\lJGF{e2G7 NWXBRVI5OzBizszN M1u1dFIxKGh? NV;DUlZNSmyxY3vzJIlvfmG|aY\lJIFkfGm4aYT5 NGi4fXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97OUOwPFczLz57OUOwPFczRC:jPh?=
CCL39 NWiwO5JETnWwY4Tpc44hSXO|YYm= NUXnU3BxOzBizszN NVPRcJU3OzBibXnu MoDZR49ueGyndHXsfUBi[m:uaYPo[ZMh[WO2aY\heIlwdiCxZjDOZU1JKGW6Y3jhcodmeiCQSFWxJIJ6KGmwdHXndolvew>? MlyyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTZ7M{O4Nkc,QTZ7M{O4Nlww[T5?
HeLa Mli0SpVv[3Srb36gRZN{[Xl? NFmx[pkyOCEQvF2= MXqzNEBucW5? MV7Jcohq[mm2czD0bIUh\m:{bXH0bY9vKG:oIIP0doV{eyCoaXLldpMh[W6mIITo[UBie3OnbXLsfUBw\iC4aX7jeYxqdi2lb370ZYlvcW6pIH\vZ4FtKGGmaHXzbY9vew>? M4\Je|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4NkiwO|IoRjl4NkiwO|I9N2F-
N1E-115 NYT4XW5zTnWwY4Tpc44hSXO|YYm= NVTXbFhrOTBizszN NV;IUXp{OiCq MlLZSG1UVw>? MmfSTY5pcWKrdIOgeIhmKGG|c3XtZox6KG:oIH3pZ5JwfHWkdXzld{BidmRiaX70[ZJu\WSrYYTlJIZqdGGvZX70d{B1dyCob4LtJIV5fGWwZHXkJJBzd2Onc4Pldy=> NVHHR3ZURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[0O|Y2PCd-OU[0O|Y2PDxxYU6=
Swiss 3T3 cells NEjOeWxHfW6ldHnvckBCe3OjeR?= NWfGTm9FOTBizszN M3TsT|IhcA>? NV\meII1TE2VTx?= MlzXTY5pcWKrdIOgeIhmKGG|c3XtZox6KG:oIH3pZ5JwfHWkdXzld{BidmRiaX70[ZJu\WSrYYTlJIZqdGGvZX70d{B1dyCob4LtJIV5fGWwZHXkJJBzd2Onc4Pldy=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86PjR5NkW0K|46PjR5NkW0QE9iRg>?

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western Blot
p-LIMK1(Thr508); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-LIMK2(Thr505); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-Cofilin(Ser3); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

CDK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

KRT7; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

ROCK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

E-cadherin; 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

p-MLC2(Ser19); 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

24704720 26555939 27399334
Transwell migration assay
cell migration inhibition ; 

PubMed: 27694793     


Data represent mean ± SEM, n=3. Compared with control, * P<0.05, ** P<0.01. Compared with control, Y-27632, or EGCG, *** P<0.01.

27694793
Immunofluorescence
Neurotoxicity Assay; 

PubMed: 21362567     


Representations of neurodegeneration in H9-, HUF5- and G2019S-iPSC derived neurons when treated with neurotoxins, H2O2 and MG-132, and ROCK inhibitor Y-27632. Scale bar = 50 µm.

E-cadherin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

beta-catenin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

Phalloidin; 

PubMed: 27399334     


Triple IF staining for F-actin (phalloidin, green) E-cadherin (b) (red) and nuclei (DAPI, blue) showed decreased F-actin, increased E-cadherin and altered cell shape and size in SW-480-FOXM1D cells treated with the ROCKs inhibitor Y-27632 or fasudil (both 10 μM, for 24 h). Scale bar, 25 μm. 

21362567 24523903 27399334
Growth inhibition assay
cell proliferation ; 

PubMed: 24523903     


MCF-7 cells and MDA-MB-231 cells were cultured on tissue culture plates for four days with or without Y-27632 (20 µM). Relative cell proliferation rates were determined by the cell number assayed using CCK-8 kit. The data are expressed as the mean ± SD. n = 3 culture dishes. The p-value was less than 0.05 for comparisons between control (Control) and treatment groups (Y-27632) in MCF-7 cells. *, p<0.05.

24523903
ELISA
caspase-9; 

PubMed: 30320378     


Caspase-9 activity was measured via ELISA. Mst1-mediated casapse-9 activation was inhibited by Y-27632 in A549 cells. *P<0.05. Ad, adenovirus; Mst1, mammalian STE20-like kinase 1.

30320378
体内試験 Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

お薦めの試験操作(参考用のみ)

動物試験:[1] [7]
- 合併
  • 動物モデル: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • 投薬量: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • 投与方法: Orally (Rat); i.p. (Mice)
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 64 mg/mL (199.83 mM) warming
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
saline
混合させたのち直ちに使用することを推奨します。
10mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 320.26
化学式

C14H21N3O.2HCl

CAS No. 129830-38-2
Storage powder
in solvent
別名 N/A
Smiles CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問(FAQ)

  • 質問1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 質問2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCKシグナル伝達経路

ROCK Inhibitors with Unique Features

相関ROCK製品