Y-27632 2HCl

製品コードS1049

Y-27632 2HCl化学構造

分子量(MW):320.26

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

サイズ 価格(税別)  
JPY 25896.00
JPY 11620.00
JPY 19920.00
JPY 28220.00
JPY 94620.00

文献中Selleckの製品使用例(53)

カスタマーフィードバック(6)

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

製品安全説明書

ROCK阻害剤の選択性比較

生物活性

製品説明 Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
ターゲット
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外試験

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells MnP4SpVv[3Srb36gRZN{[Xl? NXuyUHpYOTBizszN NXj0W|ZOOiCq MWfEUXNQ M4LybGlvcGmkaYTzJJRp\SCjc4PlcYJtgSCxZjDtbYNzd3S3YoXs[ZMh[W6mIHnueIVzdWWmaXH0[UBncWyjbXXueJMhfG9iZn;ycUBmgHSnbnTl[EBxem:lZYPz[ZM> NVW2RWV{QTZ2N{[1OC=>
N1E-115 NF;GcXNHfW6ldHnvckBCe3OjeR?= NWfTXIRbOTBizszN MUSyJIg> Mn70SG1UVw>? MnzoTY5pcWKrdIOgeIhmKGG|c3XtZox6KG:oIH3pZ5JwfHWkdXzld{BidmRiaX70[ZJu\WSrYYTlJIZqdGGvZX70d{B1dyCob4LtJIV5fGWwZHXkJJBzd2Onc4Pldy=> MYW5OlQ4PjV2
HeLa NFr2RZBHfW6ldHnvckBCe3OjeR?= NIizbpAyOCEQvF2= NWW4PFBnOzBibXnu NV7GS5Z5UW6qaXLpeJMhfGinIH\vdo1ifGmxbjDv[kB{fHKnc4Og[olj\XK|IHHu[EB1cGViYYPz[Y1jdHlib3[geolv[3WuaX6tZ49vfGGrbnnu[{Bnd2OjbDDh[Ihme2mxboO= MVe5OlY5ODd{
CCL39 NWS3U3huTnWwY4Tpc44hSXO|YYm= NHzNOlA{OCEQvF2= NV3BSldKOzBibXnu NELRXZNEd22ybHX0[Yx6KGGkb3zpd4hmeyCjY4TpeoF1cW:wIH;mJG5iNUhiZYjjbIFv\2W{IF7ISVEh[nliaX70[YdzcW6| NI\iNHI6Pjl|M{iy
Mesothelial cells from rat mesentery MmO0TY53[XOrdnWgRZN{[Xl? MYezNEDPxE1? Mk\wNlAhcA>? MlXjRoxw[2u|IHnueoF{cX[nIHHjeIl3cXS7 NIfFeXA6QTNyOEey
NIH3T3 M1rtOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfpfJU2OTBizszN MlzWNVgh\A>? MVHEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To Mn61NVAxOjF|OE[=
Dbl-d NGC0SJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXCNVAh|ryP NXHZUJNDOThiZB?= M2HPb3N1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NG\FZWkyODB{MUO4Oi=>
Dbl-e M1jGfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYixNEDPxE1? NXXabYpYOThiZB?= NXnBd4lvVW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= MmPlNVAxOjF|OE[=
mNET1-d M{HEPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWGxNEDPxE1? Mmm4NVgh\A>? M3viZnN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp M4iwflExODJzM{i2
mNET1-e Mm\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXixNEDPxE1? M1q0XFE5KGR? MVPTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MVuxNFAzOTN6Nh?=
Ras-2 NUPvOVRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWCxNEDPxE1? M{fpbVE5KGR? M1GwZXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NHnvNmcyODB{MUO4Oi=>
Ras-4 NGexNGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXnepMyOTBizszN NYfPWHlSOThiZB?= Ml6yV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NGTEeYoyODB{MUO4Oi=>
Src-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWKxNEDPxE1? MornNVgh\A>? Mm[4SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MYCxNFAzOTN6Nh?=
Src-4 MlHTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjjOXcyOCEQvF2= NF7RUIQyQCCm Mkm3SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MXmxNFAzOTN6Nh?=
NIH3T3 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVWxNEDPxE1? MWexPEBl M4HFUGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NH:yT2cyODB{MUO4Oi=>
Src-1 NWfjenlzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInUNY0yOCEQvF2= NEnn[JEyQCCm M4WwVWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MmXzNVAxOjF|OE[=
Src-2 Mo\JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjVO|BYOTBizszN NYXY[2FSOThiZB?= NULTXnZKTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NUPkTms4OTByMkGzPFY>
SW620 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fUfFExKM7:TR?= NVLoO2l7OThiZB?= NG\6SYVFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MlS1NVAxOjF|OE[=
HCT15 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIP5UWkyOCEQvF2= NXHLO|VCOThiZB?= NV[5Rpd2TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MWCxNFAzOTN6Nh?=
HCT116 NVP3PVhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\EeGIyOCEQvF2= NVq5dHUyOThiZB?= MX;TeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NFToRoEyODB{MUO4Oi=>
LS174T Mn\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M375SlExKM7:TR?= MmLLNVgh\A>? NIfLNHFOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq MXmxNFAzOTN6Nh?=
Neonatal rat ventricular myocytes NILUdXlHfW6ldHnvckBCe3OjeR?= NH;RZY0yOCEQvF2= Mn3kOFghcA>? NWDyXGJlUW6qaXLpeJMhTVRvMT3pcoR2[2WmIHnuZ5Jm[XOnczDpckBxem:2ZXnuJJN6dnSqZYPpd{wh[2WubDDzbZpmKGGwZDDtfY9ncWK{aXzsZZIhd3KpYX7pfoF1cW:w MonxNVA{QDZ4MUO=
Stellate Cell MWPGeY5kfGmxbjDBd5NigQ>? NGL1dZozPSEQvF2= MlnqNVUhdWmw NIHT[oZKdmirYnn0d{Bnd3KvYYTpc44hd2ZiRj3hZ5RqdiC|dILld5Mh\mmkZYLzJIFv\CCyaH;zdIhwenmuYYTpc44hd2ZibYnvd4lvKGyrZ3j0JINp[Wmw M2TOfVExPjByNEm2
Rat Vascular Smooth Muscle Cells NInpc45HfW6ldHnvckBCe3OjeR?= M3PWS|ExKM7:TR?= MUSyJIg> Mm[3TY5pcWKrdIOgZY5ocW:2ZX7zbY4hUUlvaX7keYNm\CCqeYDldpRzd3CqeR?= MU[xNFY1OjNzNx?=
PC3 MmThSpVv[3Srb36gRZN{[Xl? M2nuflI2KM7:TR?= Mn\LNUBp NXjFZ|RkUW6mdXPld{Bud3KyaH;sc4dq[2GuIHPoZY5o\XN? NHmzdoQyODd{MES3NS=>
PC3 NXzQTZJJVWmpcnH0bY9vKEG|c3H5 NFHtcWgzPSEQvF2= MnXKNUBp NUnHW2NwUW6qaXLpeJMhfGinIFLNSmIuS01iYX7kJJRp\SCHR1[td5RqdXWuYYTl[EBucWe{YYTpc44> M1HGW|ExPzJyNEex
PC3 NYm4XWFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV2yOUDPxE1? Mk\sNVchcA>? M33LfWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NEOyXHcyODd{MES3NS=>
LNCaP NFOwPYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\LRmYzPSEQvF2= M1;HTFE4KGh? MWjEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NWqw[oFrOTB5MkC0O|E>
Rat hepatic stellate cells MoXrSpVv[3Srb36gRZN{[Xl? NXf4N5RDOzBizszN NFS4OIg1QCCq MVTEbY1qdmm|aHXzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiRYLrNkwh[W6mIHTlZ5Jm[XOnczDu[ZchTE6DIIP5cpRp\XOrcx?= NHfmcIgyODh2NU[2Ny=>
Pancreatic acinar cells NHj6dJlHfW6ldHnvckBCe3OjeR?= NGDme2kyOMLizszN NFvEPFY4OCCvaX6= NW\FblB3WG:2ZX70bYF1\XNiQ1PLMZN1cW23bHH0[YQheGGwY4LlZZRq[yCnbor5cYUhe2WlcnX0bY9v M1TqW|EzPzR3MEiw
C2C12 NEGwdJdHfW6ldHnvckBCe3OjeR?= NIP0fncyOMLizszN MVG2JIg> NVLR[Ic5WHKndnXueJMhfGinIIPldolv\SCyaH;zdIhwenmuYYTpc44hd2ZiSWLTMVEhcW6mdXPl[EBjgSCrboP1cIlvKGGwZD;vdkBVVkZvzsG= MY[xOlI3PzF{NB?=
PC 12 MnfpSpVv[3Srb36gRZN{[Xl? MlTZNVDDqM7:TR?= NFH0SWYzPCCq NGLrfHFCfHSnboXheIV{KGOjdHXjbI9t[W2rbnWgZolwe3mwdHjld4l{ M2XmbVE3OjF7NEK0
Cynomolgus monkey embryonic stem cells MUHDfZRwfG:6aXOgRZN{[Xl? NFr0UJczOCEEtV2= MmHNNlQhcA>? MXrQdo9ud3SnczDjfWVUKGOnbHygd5Vzfmm4YXy= MoeyNVg6PDB6NUW=
TSGH 8301 NUezO4toVWmpcnH0bY9vKEG|c3H5 MYeyNEDDvU1? M2jG[|EhcA>? MUPJcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? MWixPVg6PjR5NR?=
Swiss3T3 NWTrfZlLS2:ub375MYZwem2rbnegRZN{[Xl? Moi3NVAhyrWP NVnVXY83OTNiZB?= MmmyTY5kemWjc3XzJJBzd3O2YYTlJINmdGxiY3;sc456NW[xcn3pcoch[WO2aY\peJk> NIHGTW0zOTR4NEmwNi=>
HT22 MnSyR5l1d3SxeHnjJGF{e2G7 M1[xVVExKML3TR?= MXOxN{Bp NYDNVmRiWHKxdHXjeJMh[WejaX7zeEBodHW2YX3heIUucW6mdXPl[EBv\XW{b37hcEBl\WG2aB?= NFH2eXMzOjhzMEizOS=>
Salivary gland stem cells NE\J[3RHfW6ldHnvckBCe3OjeR?= NV3nbol2OTBiwsXN MWq3JIQ> MWPS[YR2[2W|IGPHV2Mhe2WwZYPj[Y5k\Q>? NEDoO2MzPThyNEW2NC=>

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

体内試験 Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

お薦めの試験操作(参考用のみ)

動物試験:[1] [7]
+ 展開
  • 動物モデル: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • 製剤: Dissolved in DMSO, and diluted in saline (Rat); 0.9% NaCl (Mice)
  • 投薬量: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • 投与方法: Orally (Rat); i.p. (Mice)
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 64 mg/mL (199.83 mM) warming
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
saline
混合させたのち直ちに使用することを推奨します。
10mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 320.26
化学式

C14H21N3O.2HCl

CAS No. 129830-38-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問(FAQ)

  • 質問1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 質問2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID