CHIR-99021 (CT99021)

For research use only. Not for use in humans.

製品コードS1263

CHIR-99021 (CT99021)化学構造

CAS No. 252917-06-9

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.

サイズ 価格(税別) 在庫  
JPY 16900 あり
JPY 30200 あり
JPY 96600 あり
JPY 163000 あり
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バルク問合せ

文献中Selleckの製品使用例(459)

製品安全説明書

GSK-3阻害剤の選択性比較

生物活性

製品説明 CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
ターゲット
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
体外試験

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM[1].

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LOUCY NWHydXRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mle2TWM2OD1zOEKuOlg5KM7:TR?= MoXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SN12C MlnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTF6Mj64Nlkh|ryP M4rQWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
A427 NEXFcoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PITGlEPTB;MUi0MlY{OSEQvF2= MmS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
DOK M1LRUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2Kwe2lEPTB;MUi0MlY1OyEQvF2= M372WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MDA-MB-415 Mn6wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3lOYh4UUN3ME2xPFQvPjVzIN88US=> M1\SN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MRK-nu-1 M{PlTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTF6ND65O|Ih|ryP MmfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
LB2241-RCC MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDkbGlKSzVyPUG4OU4yPDVizszN NYfoO3FtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1563 NYHLXWZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3qxNGlEPTB;MUi1MlIxQSEQvF2= MmfaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MDA-MB-453 M2rjc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHOWo1KSzVyPUG4Ok46PDFizszN M1nxV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MONO-MAC-6 M3HoPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LGbGlEPTB;MUi3MlM6OiEQvF2= NGPjZ3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CAPAN-1 NVzvZVB{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojOTWM2OD1zOEeuOFU{KM7:TR?= NGP5dmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SK-LU-1 MkDOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzwfHNvUUN3ME2xPFgvPDJ6IN88US=> NVi4U4R7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1299 NELISnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYj2N5NkUUN3ME2xPFgvPzF7IN88US=> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KG-1 Ml3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvDNHg4UUN3ME2xPFkvPzl6IN88US=> NIDNWYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
ETK-1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3rVG1KSzVyPUG5NE4yQDFizszN NELDbJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NCI-H838 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkizTWM2OD1zOUCuN|Qh|ryP M4DjeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HCE-4 NYT2ZYh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTF7MD65PVIh|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
A2780 NUHNTnA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPxendKSzVyPUG5Nk45QTFizszN M4jUSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
COLO-668 MkXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTF7Mz6xOVch|ryP M4PHOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HGC-27 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLSTWM2OD1zOUWuOVM5KM7:TR?= NVjSN29NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SW1088 M1:1cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnqb4JbUUN3ME2xPVUvPjd5IN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KOSC-2 NHGyR4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXeyU|JPUUN3ME2xPVUvQDF6IN88US=> M{XLfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW780 NHviW5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHsSWtKSzVyPUG5Ok44OzRizszN MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
TE-1 M{X0Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTF7Nj6yOkDPxE1? NVTvRZFMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
22RV1 M2\RV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLL[3VKSzVyPUG5O{41OjRizszN NXr4TYNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
IA-LM MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DLO2lEPTB;MUm3MlgzOiEQvF2= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
TE-5 NYLO[IhvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrLTWM2OD1zOUiuNFM{KM7:TR?= NH3l[o09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
JVM-3 NGjTfFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jCRmlEPTB;MUm4MlQyQSEQvF2= MlrxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HT55 M4HQS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHkTWM2OD1zOUmuNVkzKM7:TR?= M4PwblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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MFM-223 NHG4WZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIf3c5hKSzVyPUKwNU42QDdizszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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LAMA-84 Mn;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPHUlJ1UUN3ME2yNFIvODd|IN88US=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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