CHIR-99021 (CT99021)

For research use only. Not for use in humans.

製品コードS1263

CHIR-99021 (CT99021)化学構造

CAS No. 252917-06-9

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.

サイズ 価格(税別)  
JPY 16900
JPY 30200
JPY 96600
JPY 163000
最寄りの販売代理店を探す

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バルク問合せ

文献中Selleckの製品使用例(489)

製品安全説明書

GSK-3阻害剤の選択性比較

生物活性

製品説明 CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
ターゲット
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
体外試験

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM[1].

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LOUCY NVfyNVA5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\TeZN6UUN3ME2xPFIvPjh6IN88US=> NYjpRVRKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SN12C NHXrbFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVGzN2RyUUN3ME2xPFIvQDJ7IN88US=> NG[1XWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
A427 NF7XN3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPUTWM2OD1zOESuOlMyKM7:TR?= NI\3S4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
DOK NXrCUYc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLyVlVCUUN3ME2xPFQvPjR|IN88US=> Mkm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MDA-MB-415 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDUTWM2OD1zOESuOlUyKM7:TR?= MkDOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MRK-nu-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTF6ND65O|Ih|ryP NFThR3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
LB2241-RCC NG\rZ5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TzPGlEPTB;MUi1MlE1PSEQvF2= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H1563 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofOTWM2OD1zOEWuNlA6KM7:TR?= Mo[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MDA-MB-453 Ml;MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTF6Nj65OFEh|ryP MoDlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MONO-MAC-6 M{foemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDndJlKSzVyPUG4O{4{QTJizszN M1XDWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
CAPAN-1 NYXp[5hFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLZb|ZMUUN3ME2xPFcvPDV|IN88US=> NHzkcZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SK-LU-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHLbnFZUUN3ME2xPFgvPDJ6IN88US=> NX7Lc3FtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1299 NXn1eId6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIW0c4NKSzVyPUG4PE44OTlizszN MkHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
KG-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2T0OmlEPTB;MUi5Mlc6QCEQvF2= NXHkdI1SRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
ETK-1 MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjvc|ZGUUN3ME2xPVAvOThzIN88US=> NHzEWJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NCI-H838 NGf2RWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnm4TWM2OD1zOUCuN|Qh|ryP MlPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HCE-4 NY\2bodKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTF7MD65PVIh|ryP NXfyN2dyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
A2780 NEC0OYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HkfGlEPTB;MUmyMlg6OSEQvF2= NFzTWo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
COLO-668 Mne1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTF7Mz6xOVch|ryP MoDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HGC-27 NVPLWW1UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEe5Z2dKSzVyPUG5OU42OzhizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SW1088 NH23fJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzVTWM2OD1zOUWuOlc4KM7:TR?= NF7zUXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
KOSC-2 MorvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrIRm83UUN3ME2xPVUvQDF6IN88US=> NWPHbpNGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SW780 M3zaNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfR[JJTUUN3ME2xPVYvPzN2IN88US=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
TE-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnSO4hKSzVyPUG5Ok4zPiEQvF2= MnXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
22RV1 M2rXe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4q3c2lEPTB;MUm3MlQzPCEQvF2= Mly2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
IA-LM M{jSZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7YVmJKSzVyPUG5O{45OjJizszN NXK4W4ZORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
TE-5 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFW4dpFKSzVyPUG5PE4xOzNizszN M17J[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
JVM-3 NW\Mb4hJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfUfYlGUUN3ME2xPVgvPDF7IN88US=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
HT55 NFTSNllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDxZVdKSzVyPUG5PU4yQTJizszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
QIMR-WIL MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTJyMD6xNVYh|ryP MoPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SJRH30 NEHONYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;lVYJKSzVyPUKwNE42OTVizszN NHPhNmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
COLO-679 NWjMcYllT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHidHh7UUN3ME2yNFAvQDV7IN88US=> NGf5XGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MFM-223 M3P1V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7xVIZkUUN3ME2yNFEvPTh5IN88US=> MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MDA-MB-231 NUjKOXF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\LTWM2OD1{MECuPVAzKM7:TR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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LAMA-84 MnuxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTJyMj6wO|Mh|ryP M4j0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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NCI-H345 NEnD[oRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjNPWg4UUN3ME2yNFMvODJ5IN88US=> M{THRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
KYSE-70 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LYbGlEPTB;MkCzMlg4QSEQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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NCI-H1651 Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3X4bmlEPTB;MkC2Mlc{KM7:TR?= NFHzeW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HPAF-II NIDKem9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPV[GJDUUN3ME2yNFkvPjdzIN88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NUGC-3 Ml3OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTJyOT62O|gh|ryP Mlr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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