Akti-1/2

別名：Akt Inhibitor VIII

製品コード：S7776 純度：99.11%

Akti-1/2 (Akt Inhibitor VIII) is a highly selective Akt1/Akt2 inhibitor with IC50 of 58 nM/210 nM, respectively, about 36-fold selectivity for Akt1 over Akt3. Akti-1/2 induces apoptosis.

CAS No. 612847-09-3

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 29500	国内在庫あり
5mg	JPY 22000	国内在庫あり
25mg	JPY 74500	国内在庫あり
100mg	JPY 145500	国内在庫なし(納期7~10日)
1g	JPY 598500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：sales@selleck.co.jp よく尋ねられる質問

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製品安全説明書

現在のバッチを見る：純度： 99.11%

99.11

Akti-1/2関連製品

関連ターゲット	Akt1 Akt2 Akt3	もっと見る
関連製品	MK-2206 Dihydrochloride Perifosine SC79 Capivasertib (AZD5363) GSK690693 Ipatasertib (GDC-0068) Triciribine (API-2) CCT128930 Afuresertib (GSK2110183) A-674563 HCl AT7867 Oridonin PHT-427 TIC10 (ONC201) Miransertib (ARQ 092) HCl AT13148 Uprosertib (GSK2141795) Miransertib (ARQ-092) SC66 Deguelin ML-9 HCl	もっと見る
関連化合物ライブラリー	Kinase Inhibitor Library PI3K/Akt Inhibitor Library Apoptosis Compound Library Cell Cycle compound library NF-κB Signaling Compound Library	もっと見る

シグナル伝達経路

Aktシグナル伝達経路

Akt阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	活性情報
ME-180 cell	Growth inhibition assay	Inhibition of human ME-180 cell growth in a cell viability assay, IC50=3.15011 Μm
NCI-H526 cells	Growth inhibition assay	Inhibition of Akt-mediated antiapoptotic activity in human NCI-H526 cells assessed as disruption of protein-Apaf-1 complex at 50 uM after 24 hrs by Western blot analysis
MDA-MB-415 cell	Growth inhibition assay	Inhibition of human MDA-MB-415 cell growth in a cell viability assay, IC50=1.73449 μM
MDA-MB-468 cell	Growth inhibition assay	Inhibition of human MDA-MB-468 cell growth in a cell viability assay, IC50=3.70773 μM
IGROV-1 cell	Growth inhibition assay	Inhibition of human IGROV-1 cell growth in a cell viability assay, IC50=0.72328 Μm
MDA-MB-361 cell	Growth inhibition assay	Inhibition of human MDA-MB-361 cell growth in a cell viability assay, IC50=0.48995 μM
HCC70 cell	Growth inhibition assay	Inhibition of human HCC70 cell growth in a cell viability assay, IC50=0.75078 μM
HGC-27 cell	Growth inhibition assay	Inhibition of human HGC-27 cell growth in a cell viability assay, IC50=0.15033 μM
ETK-1	Growth inhibition assay	Inhibition of human ETK-1 cell growth in a cell viability assay, IC50=4.57383 μM
EFM-19 cell	Growth inhibition assay	Inhibition of human EFM-19 cell growth in a cell viability assay, IC50=0.56408 μM
EFO-21 cell	Growth inhibition assay	Inhibition of human EFO-21 cell growth in a cell viability assay, IC50=4.43468 μM
ECC12 cell	Growth inhibition assay	Inhibition of human ECC12 cell growth in a cell viability assay, IC50=8.88569 μM
DSH1 cell	Growth inhibition assay	Inhibition of human DSH1 cell growth in a cell viability assay, IC50=0.98722 μM
DOK cell	Growth inhibition assay	Inhibition of human DOK cell growth in a cell viability assay, IC50=4.15134 μM
DMS-273 cell	Growth inhibition assay	Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=4.15134 μM
Calu-3 cell	Growth inhibition assay	Inhibition of human Calu-3 cell growth in a cell viability assay, IC50=3.65321 μM
Calu-1 cell	Growth inhibition assay	Inhibition of human Calu-1 cell growth in a cell viability assay, IC50=7.25613 μM
Ca9-22 cell	Growth inhibition assay	Inhibition of human Ca9-22 cell growth in a cell viability assay, IC50=5.97333 μM
Ca-Ski cell	Growth inhibition assay	Inhibition of human Ca-Ski cell growth in a cell viability assay, IC50=5.3411 μM
CTB-1 cell	Growth inhibition assay	Inhibition of human CTB-1 cell growth in a cell viability assay, IC50=7.59751 μM
COR-L279 cell	Growth inhibition assay	Inhibition of human COR-L279 cell growth in a cell viability assay, IC50=1.68064 μM
COLO-205 cell	Growth inhibition assay	Inhibition of human COLO-205 cell growth in a cell viability assay, IC50=1.99706 μM
CHP-212 cell	Growth inhibition assay	Inhibition of human CHP-212 cell growth in a cell viability assay, IC50=4.55867 μM
CESS cell	Growth inhibition assay	Inhibition of human CESS cell growth in a cell viability assay, IC50=2.70089 μM
CCRF-CEM cell	Growth inhibition assay	Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=4.93541 μM
CCF-STTG1 cell	Growth inhibition assay	Inhibition of human CCF-STTG1 cell growth in a cell viability assay, IC50=6.81501 μM
YKG-1 cell	Growth inhibition assay	Inhibition of human YKG-1 cell growth in a cell viability assay, IC50=1.01806 μM
VMRC-RCZ cell	Growth inhibition assay	Inhibition of human VMRC-RCZ cell growth in a cell viability assay, IC50=3.70955 μM
UACC-893 cell	Growth inhibition assay	Inhibition of human UACC-893 cell growth in a cell viability assay, IC50=5.29339 μM
UACC-62 cell	Growth inhibition assay	Inhibition of human UACC-62 cell growth in a cell viability assay, IC50=27.0545 μM
U251 cell	Growth inhibition assay	Inhibition of human U251 cell growth in a cell viability assay, IC50=19.204 μM
U-698-M cell	Growth inhibition assay	Inhibition of human U-698-M cell growth in a cell viability assay, IC50=39.0015 μM
U-118-MG cell	Growth inhibition assay	Inhibition of human U-118-MG cell growth in a cell viability assay, IC50=9.90258 μM
TT cell	Growth inhibition assay	Inhibition of human TT cell growth in a cell viability assay, IC50=5.32724 μM
TE-8 cell	Growth inhibition assay	Inhibition of human TE-8 cell growth in a cell viability assay, IC50=24.5243 μM
TE-5 cell	Growth inhibition assay	Inhibition of human TE-5 cell growth in a cell viability assay, IC50=4.82452 μM
TE-15 cell	Growth inhibition assay	Inhibition of human TE-15 cell growth in a cell viability assay, IC50=6.49393 μM
TE-10 cell	Growth inhibition assay	Inhibition of human TE-10 cell growth in a cell viability assay, IC50=9.04225 μM
CAL-85-1 cell	Growth inhibition assay	Inhibition of human CAL-85-1 cell growth in a cell viability assay, IC50=5.95309 μM
CAL-54 cell	Growth inhibition assay	Inhibition of human CAL-54 cell growth in a cell viability assay, IC50=4.88625 μM
CAL-51 cell	Growth inhibition assay	Inhibition of human CAL-51 cell growth in a cell viability assay, IC50=7.62841 Μm
CAL-33 cell	Growth inhibition assay	Inhibition of human CAL-33 cell growth in a cell viability assay, IC50=3.43125 μM
C3A cell	Growth inhibition assay	Inhibition of human C3A cell growth in a cell viability assay, IC50=4.42192 μM
C2BBe1 cell	Growth inhibition assay	Inhibition of human C2BBe1 cell growth in a cell viability assay, IC50=13.6679 μM
C-33-A cell	Growth inhibition assay	Inhibition of human C-33-A cell growth in a cell viability assay, IC50=12.4783 μM
BxPC-3 cell	Growth inhibition assay	Inhibition of human BxPC-3 cell growth in a cell viability assay, IC50=2.22442 μM
BB30-HNC cell	Growth inhibition assay	Inhibition of human BB30-HNC cell growth in a cell viability assay, IC50=2.86449 μM
AU565 cell	Growth inhibition assay	Inhibition of human AU565 cell growth in a cell viability assay, IC50=1.23917 μM
AN3-CA cell	Growth inhibition assay	Inhibition of human AN3-CA cell growth in a cell viability assay, IC50=1.65611 μM
A498 cell	Growth inhibition assay	Inhibition of human A498 cell growth in a cell viability assay, IC50=1.08134 μM
A427 cell	Growth inhibition assay	Inhibition of human A427 cell growth in a cell viability assay, IC50=3.07693 μM
A4-Fuk cell	Growth inhibition assay	Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=1.8583 μM
8-MG-BA cell	Growth inhibition assay	Inhibition of human 8-MG-BA cell growth in a cell viability assay, IC50=8.63401 μM
769-P cell	Growth inhibition assay	Inhibition of human 769-P cell growth in a cell viability assay, IC50=1.10483 μM
697 cell	Growth inhibition assay	Inhibition of human 697 cell growth in a cell viability assay, IC50=7.17746 μM
639-V cell	Growth inhibition assay	Inhibition of human 639-V cell growth in a cell viability assay, IC50=5.8773 μM
23132-87 cell	Growth inhibition assay	Inhibition of human 23132-87 cell growth in a cell viability assay, IC50=0.75938 μM
22RV1 cell	Growth inhibition assay	Inhibition of human 22RV1 cell growth in a cell viability assay, IC50=6.61424 μM
T47D cell	Growth inhibition assay	Inhibition of human T47D cell growth in a cell viability assay, IC50=0.5162 μM
SW954 cell	Growth inhibition assay	Inhibition of human SW954 cell growth in a cell viability assay, IC50=5.85783 μM
SW872 cell	Growth inhibition assay	Inhibition of human SW872 cell growth in a cell viability assay, IC50=14.1435 μM
SW780 cell	Growth inhibition assay	Inhibition of human SW780 cell growth in a cell viability assay, IC50=4.89358 μM
SW1710 cell	Growth inhibition assay	Inhibition of human SW1710 cell growth in a cell viability assay, IC50=1.38892 μM
SU-DHL-1 cell	Growth inhibition assay	Inhibition of human SU-DHL-1 cell growth in a cell viability assay, IC50=6.81543 μM
SR cell	Growth inhibition assay	Inhibition of human SR cell growth in a cell viability assay, IC50=13.0746 μM
SNU-C2B cell	Growth inhibition assay	Inhibition of human SNU-C2B cell growth in a cell viability assay, IC50=22.2673 μM
SNU-C1 cell	Growth inhibition assay	Inhibition of human SNU-C1 cell growth in a cell viability assay, IC50=2.00948 μM
SNU-423 cell	Growth inhibition assay	Inhibition of human SNU-423 cell growth in a cell viability assay, IC50=5.94933 μM
SNU-387 cell	Growth inhibition assay	Inhibition of human SNU-387 cell growth in a cell viability assay, IC50=4.19544 μM
SNB75 cell	Growth inhibition assay	Inhibition of human SNB75 cell growth in a cell viability assay, IC50=7.8297 μM
human SN12C cell	Growth inhibition assay	Inhibition of human SN12C cell growth in a cell viability assay, IC50=17.1967 μM
SK-MES-1 cell	Growth inhibition assay	Inhibition of human SK-MES-1 cell growth in a cell viability assay, IC50=2.10096 μM
SK-MEL-3 cell	Growth inhibition assay	Inhibition of human SK-MEL-3 cell growth in a cell viability assay, IC50=21.4696 μM
SK-MEL-24 cell	Growth inhibition assay	Inhibition of human SK-MEL-24 cell growth in a cell viability assay, IC50=13.1675 μM
SK-LU-1 cell	Growth inhibition assay	Inhibition of human SK-LU-1 cell growth in a cell viability assay, IC50=42.043 μM
SK-LMS-1 cell	Growth inhibition assay	Inhibition of human SK-LMS-1 cell growth in a cell viability assay, IC50=8.76452 μM
SK-CO-1 cell	Growth inhibition assay	Inhibition of human SK-CO-1 cell growth in a cell viability assay, IC50=10.6394 μM
SJSA-1 cell	Growth inhibition assay	Inhibition of human SJSA-1 cell growth in a cell viability assay, IC50=36.9072 μM
SIG-M5 cell	Growth inhibition assay	Inhibition of human SIG-M5 cell growth in a cell viability assay, IC50=12.6604 μM
SF295 cell	Growth inhibition assay	Inhibition of human SF295 cell growth in a cell viability assay, IC50=13.6183 μM
SF268 cell	Growth inhibition assay	Inhibition of human SF268 cell growth in a cell viability assay, IC50=15.1387 μM
SF126 cell	Growth inhibition assay	Inhibition of human SF126 cell growth in a cell viability assay, IC50=10.6746 μM
SCC-9 cell	Growth inhibition assay	Inhibition of human SCC-9 cell growth in a cell viability assay, IC50=3.08536 μM
SCC-4 cell	Growth inhibition assay	Inhibition of human SCC-4 cell growth in a cell viability assay, IC50=9.59283 μM
SCC-25 cell	Growth inhibition assay	Inhibition of human SCC-25 cell growth in a cell viability assay, IC50=15.8179 μM
SBC-5 cell	Growth inhibition assay	Inhibition of human SBC-5 cell growth in a cell viability assay, IC50=20.8325 μM
RXF393 cell	Growth inhibition assay	Inhibition of human RXF393 cell growth in a cell viability assay, IC50=20.5863 μM
RVH-421 cell	Growth inhibition assay	Inhibition of human RVH-421 cell growth in a cell viability assay, IC50=32.3606 μM
RT-112 cell	Growth inhibition assay	Inhibition of human RT-112 cell growth in a cell viability assay, IC50=6.71952 μM
RS4-11 cell	Growth inhibition assay	Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=6.68645 μM
RPMI-8402 cell	Growth inhibition assay	Inhibition of human RPMI-8402 cell growth in a cell viability assay, IC50=16.343 μM
RPMI-2650 cell	Growth inhibition assay	Inhibition of human RPMI-2650 cell growth in a cell viability assay, IC50=5.77947 μM
RO82-W-1 cell	Growth inhibition assay	Inhibition of human RO82-W-1 cell growth in a cell viability assay, IC50=47.2783 μM
RKO cell	Growth inhibition assay	Inhibition of human RKO cell growth in a cell viability assay, IC50=19.4439 μM
RERF-LC-MS cell	Growth inhibition assay	Inhibition of human RERF-LC-MS cell growth in a cell viability assay, IC50=26.1347 μM
REH cell	Growth inhibition assay	Inhibition of human REH cell growth in a cell viability assay, IC50=11.4883 μM
RD cell	Growth inhibition assay	Inhibition of human RD cell growth in a cell viability assay, IC50=20.6036 μM
RCC10RGB cell	Growth inhibition assay	Inhibition of human RCC10RGB cell growth in a cell viability assay, IC50=8.6567 μM
PF-382 cell	Growth inhibition assay	Inhibition of human PF-382 cell growth in a cell viability assay, IC50=11.6828 μM
PC-3 cell	Growth inhibition assay	Inhibition of human PC-3 cell growth in a cell viability assay, IC50=10.5135 μM
PANC-10-05 cell	Growth inhibition assay	Inhibition of human PANC-10-05 cell growth in a cell viability assay, IC50=14.9959 μM
PANC-03-27 cell	Growth inhibition assay	Inhibition of human PANC-03-27 cell growth in a cell viability assay, IC50=8.71082 μM
PA-1 cell	Growth inhibition assay	Inhibition of human PA-1 cell growth in a cell viability assay, IC50=9.07615 μM
P30-OHK cell	Growth inhibition assay	Inhibition of human P30-OHK cell growth in a cell viability assay, IC50=4.536 μM
P12-ICHIKAWA cell	Growth inhibition assay	Inhibition of human P12-ICHIKAWA cell growth in a cell viability assay, IC50=14.7683 μM
OVCAR-4 cell	Growth inhibition assay	Inhibition of human OVCAR-4 cell growth in a cell viability assay, IC50=30.466 μM
OVCAR-3 cell	Growth inhibition assay	Inhibition of human OVCAR-3 cell growth in a cell viability assay, IC50=1.84415 μM
OS-RC-2 cell	Growth inhibition assay	Inhibition of human OS-RC-2 cell growth in a cell viability assay, IC50=8.52307 μM
OMC-1 cell	Growth inhibition assay	Inhibition of human OMC-1 cell growth in a cell viability assay, IC50=9.25098 μM
OE33 cell	Growth inhibition assay	Inhibition of human OE33 cell growth in a cell viability assay, IC50=1.88926 μM
OE19 cell	Growth inhibition assay	Inhibition of human OE19 cell growth in a cell viability assay, IC50=7.76684 μM
OCUB-M cell	Growth inhibition assay	Inhibition of human OCUB-M cell growth in a cell viability assay, IC50=7.72152 μM
OCI-AML2 cell	Growth inhibition assay	Inhibition of human OCI-AML2 cell growth in a cell viability assay, IC50=33.2531 μM
OAW-42 cell	Growth inhibition assay	Inhibition of human OAW-42 cell growth in a cell viability assay, IC50=7.31565 μM
NY cell	Growth inhibition assay	Inhibition of human NY cell growth in a cell viability assay, IC50=31.1864 μM
NUGC-3 cell	Growth inhibition assay	Inhibition of human NUGC-3 cell growth in a cell viability assay, IC50=16.5906 μM
NOS-1 cell	Growth inhibition assay	Inhibition of human NOS-1 cell growth in a cell viability assay, IC50=1.36953 μM
NMC-G1 cell	Growth inhibition assay	Inhibition of human NMC-G1 cell growth in a cell viability assay, IC50=42.6693 μM
NKM-1 cell	Growth inhibition assay	Inhibition of human NKM-1 cell growth in a cell viability assay, IC50=2.75923 μM
NH-12 cell	Growth inhibition assay	Inhibition of human NH-12 cell growth in a cell viability assay, IC50=0.60864 μM
NCI-N87 cell	Growth inhibition assay	Inhibition of human NCI-N87 cell growth in a cell viability assay, IC50=29.3042 μM
NCI-H838 cell	Growth inhibition assay	Inhibition of human NCI-H838 cell growth in a cell viability assay, IC50=31.4118 μM
NCI-H747 cell	Growth inhibition assay	Inhibition of human NCI-H747 cell growth in a cell viability assay, IC50=7.62799 μM
NCI-H661 cell	Growth inhibition assay	Inhibition of human NCI-H661 cell growth in a cell viability assay, IC50=5.42326 μM
NCI-H650 cell	Growth inhibition assay	Inhibition of human NCI-H650 cell growth in a cell viability assay, IC50=7.59877 μM
NCI-H64 cell	Growth inhibition assay	Inhibition of human NCI-H64 cell growth in a cell viability assay, IC50=12.5431 μM
NCI-H630 cell	Growth inhibition assay	Inhibition of human NCI-H630 cell growth in a cell viability assay, IC50=5.51055 μM
NCI-H596	Growth inhibition assay	Inhibition of human NCI-H596 cell growth in a cell viability assay, IC50=17.5708 μM
NCI-H526 cell	Growth inhibition assay	Inhibition of human NCI-H526 cell growth in a cell viability assay, IC50=22.8873μM
NCI-H522 cell	Growth inhibition assay	Inhibition of human NCI-H522 cell growth in a cell viability assay, IC50=11.0874 μM
NCI-H520 cell	Growth inhibition assay	Inhibition of human NCI-H520 cell growth in a cell viability assay, IC50=1.13303 μM
NCI-H460 cell	Growth inhibition assay	Inhibition of human NCI-H460 cell growth in a cell viability assay, IC50=4.62993 μM
NCI-H358 cell	Growth inhibition assay	Inhibition of human NCI-H358 cell growth in a cell viability assay, IC50=19.7233 μM
NCI-H345 cell	Growth inhibition assay	Inhibition of human NCI-H345 cell growth in a cell viability assay, IC50=16.2706 μM
NCI-H292 cell	Growth inhibition assay	Inhibition of human NCI-H292 cell growth in a cell viability assay, IC50=6.04837 μM
NCI-H2452 cell	Growth inhibition assay	Inhibition of human NCI-H2452 cell growth in a cell viability assay, IC50=26.6594 μM
NCI-H2405 cell	Growth inhibition assay	Inhibition of human NCI-H2405 cell growth in a cell viability assay, IC50=10.4212 μM
NCI-H2347 cell	Growth inhibition assay	Inhibition of human NCI-H2347 cell growth in a cell viability assay, IC50=16.1581 μM
NCI-H2342 cell	Growth inhibition assay	Inhibition of human NCI-H2342 cell growth in a cell viability assay, IC50=0.93779 μM
NCI-H2228 cell	Growth inhibition assay	Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=2.28604 μM
NCI-H2196 cell	Growth inhibition assay	Inhibition of human NCI-H2196 cell growth in a cell viability assay, IC50=24.8003 μM
NCI-H2170 cell	Growth inhibition assay	Inhibition of human NCI-H2170 cell growth in a cell viability assay, IC50=18.1028 μM
NCI-H2126 cell	Growth inhibition assay	Inhibition of human NCI-H2126 cell growth in a cell viability assay, IC50=12.3371 μM
NCI-H2122 cell	Growth inhibition assay	Inhibition of human NCI-H2122 cell growth in a cell viability assay, IC50=10.1385 μM
NCI-H2030 cell	Growth inhibition assay	Inhibition of human NCI-H2030 cell growth in a cell viability assay, IC50=1.94483 μM
NCI-H2009 cell	Growth inhibition assay	Inhibition of human NCI-H2009 cell growth in a cell viability assay, IC50=7.39019 μM
NCI-H1993 cell	Growth inhibition assay	Inhibition of human NCI-H1993 cell growth in a cell viability assay, IC50=22.615 μM
NCI-H1963 cell	Growth inhibition assay	Inhibition of human NCI-H1963 cell growth in a cell viability assay, IC50=19.7494 μM
NCI-H1792 cell	Growth inhibition assay	Inhibition of human NCI-H1792 cell growth in a cell viability assay, IC50=29.8379 μM
NCI-H1770 cell	Growth inhibition assay	Inhibition of human NCI-H1770 cell growth in a cell viability assay, IC50=34.1793 μM
NCI-H1755 cell	Growth inhibition assay	Inhibition of human NCI-H1755 cell growth in a cell viability assay, IC50=1.05531 μM
NCI-H1734 cell	Growth inhibition assay	Inhibition of human NCI-H1734 cell growth in a cell viability assay, IC50=10.7788 μM
NCI-H1703 cell	Growth inhibition assay	Inhibition of human NCI-H1703 cell growth in a cell viability assay, IC50=15.577 μM
NCI-H1651 cell	Growth inhibition assay	Inhibition of human NCI-H1651 cell growth in a cell viability assay, IC50=16.4338 μM
NCI-H1650 cell	Growth inhibition assay	Inhibition of human NCI-H1650 cell growth in a cell viability assay, IC50=11.5134 μM
NCI-H1648 cell	Growth inhibition assay	Inhibition of human NCI-H1648 cell growth in a cell viability assay, IC50=5.7551 μM
NCI-H1623 cell	Growth inhibition assay	Inhibition of human NCI-H1623 cell growth in a cell viability assay, IC50=15.0596 μM
NCI-H1618 cel	Growth inhibition assay	Inhibition of human NCI-H1618 cell growth in a cell viability assay, IC50=21.9982 μM
ES7 cell	Growth inhibition assay	Inhibition of human ES7 cell growth in a cell viability assay, IC50=1.34511 μM
EW-3 cell	Growth inhibition assay	Inhibition of human EW-3 cell growth in a cell viability assay, IC50=5.64414 μM
EB2 cell	Growth inhibition assay	Inhibition of human EB2 cell growth in a cell viability assay, IC50=7.75468 μM
DoTc2-4510 cell	Growth inhibition assay	Inhibition of human DoTc2-4510 cell growth in a cell viability assay, IC50=3.43202 μM
Detroit562 cell	Growth inhibition assay	Inhibition of human Detroit562 cell growth in a cell viability assay, IC50=4.79465 μM
DV-90 cell	Growth inhibition assay	Inhibition of human DV-90 cell growth in a cell viability assay, IC50=6.03122 μM
NCI-H1563 cell	Growth inhibition assay	Inhibition of human NCI-H1563 cell growth in a cell viability assay, IC50=0.54246 μM
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生物活性

製品説明

Akti-1/2 (Akt Inhibitor VIII) is a highly selective Akt1/Akt2 inhibitor with IC50 of 58 nM/210 nM, respectively, about 36-fold selectivity for Akt1 over Akt3. Akti-1/2 induces apoptosis.

Targets

Akt1 ^[1] (Cell-free assay)	Akt2 ^[1] (Cell-free assay)	Akt3 ^[1] (Cell-free assay)
58 nM	210 nM	2119 nM

In Vitro
In vitro	In a cell-based IPKA (C33A) assay, Akti-1/2 inhibits Akt1 and Akt2 with IC50 of 305 nM and 2086 nM, respectively. In HT29, MCF7, and A2780 cells, this compound induces cell apoptosis by dramatically increasing caspase-3 activity. ^[1] In liver cells, this chemical blocks regulation of PEPCK, G6Pase expression, and FOXO1 activity. ^[2] It also strongly potentiates PAR-1-mediated platelet aggregation by blocking PKB. ^[3] In HCC827, NCI-H522, NCI-1651, and PC-9 cells, this inhibitor inhibits cell growth with IC50 of 4.7 μM, 7.25 μM, and 9.5 μM, when in combination, it causes enhanced inhibition of cell growth and apoptosis. ^[4]
Kinase Assay	Kinase screen
Kinase Assay	Briefly, all assays (25.5 μl at 21°C for 30 min) are performed using a Biomek 2000 Laboratory Automation Workstation in a 96-well format. Reactions contains 5–20 mU purified kinase along with substrate peptide or protein and are initiated by the addition of 10 mM MgAcetate and 5, 20, or 50 μM ATP ([γ-³³P]-ATP, 800 cpm/pmol).
細胞実験	細胞株	HCC827, NCI-H522, NCI-1651, and PC-9 cells
	濃度	~10 μM
	反応時間	96 h
	実験の流れ	The cell growth inhibitory effect of AKTi-1/2 is studied using the 96 h sulforhodamine B assay (SRB). Drug concentrations that inhibited 50% of cell growth (IC50) are calculated for each compound in GraphPad Prism 6.0 using non-linear regression analysis and sigmoidal dose–response (variable slope) equation.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	p-AKT / AKT / p-Foxo3a / p-GSK3β / p-p70S6K / p-S6 / p-4EBP1 / Cyclin D1 / Cyclin D2 / Cyclin D3 / p-Rb / p27		18725974
	Immunofluorescence	MCM2 / H3K9me2 / CD63		26637368

In Vivo
In Vivo	In mice, Akti-1/2 (50 mg/kg, i.p.) inhibits basal and IGF-stimulated Akt1 and Akt2 phosphorylation in lung. ^[1]
動物実験	動物モデル	C57BL/6 J mice
	投与量	50 mg/kg
	投与経路	i.p.

参考
https://pubmed.ncbi.nlm.nih.gov/15664853/ https://pubmed.ncbi.nlm.nih.gov/17563061/ https://pubmed.ncbi.nlm.nih.gov/18761726/ https://pubmed.ncbi.nlm.nih.gov/24957682/ https://pubmed.ncbi.nlm.nih.gov/34285232/ + 展開

参考

https://pubmed.ncbi.nlm.nih.gov/15664853/
https://pubmed.ncbi.nlm.nih.gov/17563061/
https://pubmed.ncbi.nlm.nih.gov/18761726/

https://pubmed.ncbi.nlm.nih.gov/24957682/
https://pubmed.ncbi.nlm.nih.gov/34285232/

+ 展開

化学情報

分子量	551.64	化学式	C₃₄H₂₉N₇O
CAS No.	612847-09-3	SDF	Download Akti-1/2 SDFをダウンロードする
Smiles	C1CN(CCC1N2C3=CC=CC=C3NC2=O)CC4=CC=C(C=C4)C5=C(N=C6C=C7C(=NC=N7)C=C6N5)C8=CC=CC=C8
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	１ケ月-20°Cin solvent

In vitro
Batch:

DMSO : 22 mg/mL ( (39.88 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo Batch: Add solvents to the product individually and in order.				投与溶液組成計算機
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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Handling Instructions

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C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):