Torin 1

製品コード:S2827

For research use only.

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

Torin 1化学構造

CAS No. 1222998-36-8

サイズ 価格(税別)
JPY 30200
JPY 113200
JPY 271200

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(174)

製品安全説明書

mTOR阻害剤の選択性比較

相関mTOR製品

生物活性

製品説明 Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
ターゲット
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
もっとクリックする
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
体外試験

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITyeJNKSzVyPUCuNFAxOzB4IN88US=> MoHFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
OCUB-M NV;RTZdST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojRTWM2OD1yLkCwNVk1KM7:TR?= M2rxdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
DOHH-2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTBwMECyNlUh|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
NOS-1 NYjaNIN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PmNWlEPTB;MD6wNFI1OiEQvF2= NI\5OlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
L-363 M{nMbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMECyPFQh|ryP Mo\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
RL95-2 NHPBSHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTBwMECzNkDPxE1? Mnv0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
BL-70 NV;ocGVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLUW5JKSzVyPUCuNFA{OzdizszN NGfWOYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
TGBC1TKB MojDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mof0TWM2OD1yLkCwN|c6KM7:TR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
697 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\XUJVKSzVyPUCuNFA3OzRizszN MmPyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
RPMI-8226 NFfGZm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\PTWM2OD1yLkCwOlUyKM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
BL-41 NXPWT3psT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfTTWM2OD1yLkCwOlk3KM7:TR?= NYHOOnNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MRK-nu-1 M2fpZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTBwMEC3NFch|ryP NFrmXG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
EW-1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwMEC3OFkh|ryP NHe0Umo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
EVSA-T NWL0NYgzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;lTWM2OD1yLkCwPFkzKM7:TR?= M{jHO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
HUTU-80 MlrnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13oVmlEPTB;MD6wNFkzQSEQvF2= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
VA-ES-BJ MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmP4TWM2OD1yLkCwPVg1KM7:TR?= MmLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
GCIY NIPzb2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTBwMEGwNFch|ryP Mn7nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
ES6 NWnoWHNVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTBwMEGwPFMh|ryP MmDhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
NALM-6 M4K5eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vGOWlEPTB;MD6wNVExOiEQvF2= M3ewdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
LB996-RCC M3e3Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1z3OGlEPTB;MD6wNVE{PyEQvF2= NHHLe489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
EW-16 NFHj[XNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDBSZJYUUN3ME2wMlAyOTl{IN88US=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
KALS-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXuTWM2OD1yLkCxNVk5KM7:TR?= NH;3b|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
OPM-2 NYfNfVM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3xRYlKSzVyPUCuNFEzQSEQvF2= NVLpOnlTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
KU812 MoKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3X0VmlEPTB;MD6wNVI6PiEQvF2= M1LBdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
A4-Fuk M4fyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTBwMEGzNVkh|ryP NELNVGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
KGN MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\2cmlEPTB;MD6wNVM2QSEQvF2= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
KS-1 NUPnO2xbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MomxTWM2OD1yLkCxN|kzKM7:TR?= NGf1UWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
no-11 NEfLb3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3CTWM2OD1yLkCxN|k2KM7:TR?= NFLC[Wo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
PSN1 MlnGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nNfmlEPTB;MD6wNVQxQSEQvF2= M1rlOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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MHH-PREB-1 M3r5d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTBwMEG4NVgh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
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KURAMOCHI NV\hR41qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTBwMEK0NVQh|ryP MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
HD-MY-Z NGLyfWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHNemtZUUN3ME2wMlAzPDJ4IN88US=> M3LLeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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LB1047-RCC NWTOeWNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTBwMEK0PFgh|ryP NUfWfYszRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
TE-12 M{HOWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;VR|JKSzVyPUCuNFI2PSEQvF2= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
MZ7-mel M1K3e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTBwMEK2NFkh|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
MMAC-SF MoXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTBwMEK2N|Mh|ryP M2LWZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
A101D NGm5e|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTBwMEK2OUDPxE1? M3XDe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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MC116 NIK4U|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTBwMEK2PEDPxE1? NFLFemU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10 NEDXN5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfCdGI{UUN3ME2wMlAzPzVizszN MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
ATN-1 M1TJZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTBwMEK3PFkh|ryP NWjobnBFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MFH-ino MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;6OnNnUUN3ME2wMlAzQDF{IN88US=> MkmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
DEL NYL2cHpmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13E[GlEPTB;MD6wNlgyPSEQvF2= NHK1bI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
OCI-AML2 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LVV2lEPTB;MD6wNlg4OSEQvF2= M4XNSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
LXF-289 NWLRNHJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTBwMEK4O|Ih|ryP NVLrSJJSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
IA-LM MnPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjWUoxKSzVyPUCuNFI6OTdizszN NV3P[ZZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
EoL-1-cell MkTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPDb2VOUUN3ME2wMlAzQTl4IN88US=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
OVCAR-4 NFPLOIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTlTWM2OD1yLkCzNFk1KM7:TR?= M3v3RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
ES1 M{XOe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX2zXFVVUUN3ME2wMlA{ODl5IN88US=> MmPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
CESS MnPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGq0eYJKSzVyPUCuNFMxQTlizszN NUn0b5JCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
GAK M3TRb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFu2eIdKSzVyPUCuNFMyQDZizszN NWixV2RYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
NTERA-S-cl-D1 NVLONW94T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1mzbmlEPTB;MD6wN|IzPiEQvF2= NYjLTGo2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
TE-10 MoLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoWyTWM2OD1yLkCzN|EyKM7:TR?= NUPzSXlkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
CCRF-CEM MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnLbFBKSzVyPUCuNFM{PzFizszN M3TkelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
NMC-G1 NXvuUmdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnUTWM2OD1yLkCzOFQ5KM7:TR?= NV\hdldqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
HOP-62 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7zdmJKSzVyPUCuNFM2PTZizszN MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
SH-4 MkfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYm2Zm9wUUN3ME2wMlA{PTh{IN88US=> NHfObIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NCI-H1648 NHjSOmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XoWWlEPTB;MD6wN|YzOiEQvF2= NXzxVJZ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
EB-3 M1fXUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TTRWlEPTB;MD6wN|Y1OyEQvF2= NGrLW2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
BB65-RCC MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnniTWM2OD1yLkCzOlkyKM7:TR?= MkPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
BE-13 NF7uPVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TLWWlEPTB;MD6wN|c2QSEQvF2= NIKxUGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
CP67-MEL NYnGS3doT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLaOnFPUUN3ME2wMlA{PzV7IN88US=> Mlj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
SW954 NVztUnB7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTBwMEO3PUDPxE1? M4nlTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
TE-5 Mn7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXZTlgzUUN3ME2wMlA{QDV2IN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
HCE-4 MlntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfvcGlKSzVyPUCuNFM5PTVizszN NVzFZXlIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
LC-1F NFHhUFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnhbIRKSzVyPUCuNFM6QDlizszN MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
TK10 MlPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfPeZJbUUN3ME2wMlA1ODF7IN88US=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
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アッセイ
Methods Test Index PMID
Western blot ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b ; LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ; p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 ; Vinculin / p-S6 28494858 24126619 21965657 28757207
Immunofluorescence lipin-1 28347999
Growth inhibition assay Cell viability 28757207
体内試験 Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

お薦めの試験操作(参考用のみ)

キナーゼ試験:[1]
  • mTORC1 and mTORC2 in Vitro Kinase Assays:

    To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.

細胞試験:[1]
  • 細胞株:MEFs
  • 濃度: ~250 nM
  • 反応時間:4 days
  • 実験の流れ:Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
動物試験:[2]
  • 動物モデル:U87MG xenograft model
  • 投薬量:20 mg/kg
  • 投与方法:Administered via i.p. once daily

溶解度 (25°C)

体外

化学情報

分子量 607.62
化学式

C35H28F3N5O2

CAS No. 1222998-36-8
Storage 3年 -20°C
2年 -80°C in solvent
Smiles CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

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よくある質問(FAQ)

質問1:
How to re-constitute the inhibitor for i.p. administration?

回答
S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

Tags: Torin 1を買う | Torin 1 ic50 | Torin 1供給者 | Torin 1を購入する | Torin 1費用 | Torin 1生産者 | オーダーTorin 1 | Torin 1化学構造 | Torin 1分子量 | Torin 1代理店