Azacitidine (5-Azacytidine)

For research use only. Not for use in humans.

製品コードS1782 別名：5-AzaC,Ladakamycin, AZA,5-Aza, CC-486

Azacitidine (5-Azacytidine)化学構造

CAS No. 320-67-2

Azacitidine (5-Azacytidine, 5-AzaC, Ladakamycin, AZA, 5-Aza, CC-486) is a nucleoside analogue of cytidine that specifically inhibits DNA methylation by trapping DNA methyltransferases. Azacitidine induces mitochondrial apoptosis and autophagy.

サイズ 価格(税別)  
10mM (1mL in DMSO) JPY 23500
JPY 18100
JPY 40800
最寄りの販売代理店を探す

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バルク問合せ

文献中Selleckの製品使用例(70)

製品安全説明書

DNA Methyltransferase阻害剤の選択性比較

生物活性

製品説明 Azacitidine (5-Azacytidine, 5-AzaC, Ladakamycin, AZA, 5-Aza, CC-486) is a nucleoside analogue of cytidine that specifically inhibits DNA methylation by trapping DNA methyltransferases. Azacitidine induces mitochondrial apoptosis and autophagy.
ターゲット
DNA methyltransferase [1]
(Cell-free assay)
体外試験

Azacitidine is widely used to demonstrate the correlation between loss of methylation in specifc gene regions and activation of the associated genes. After incorporation into DNA, Azacitidine inhibits DNA methyltransferase noncompetitively, causing a block in cytosine methylation in newly replicated DNA but not in resting, nondividing cells. [1] Azacitidine induces differentiation of Friend Erythroleukemia Cell C3H10T1/2 with myotube formation. [2] Azacitidine can be activated to the nucleoside triphosphate and incorporate into both DNA and RNA, leading to inhibition of DNA, RNA and protein synthesis in normal eukaryotic cells and in cancer cell lines, which could finally leads to cell death. Azacitidine also inhibits the incorporation of purine metabolites into macromolecules. Azacitidine inhibits the L1210 cells growth with IC50 and of 0.019 μg/mL. [3]
細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Raji  MnfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkThNE4yNTVyIN88US=> NH;UeYcyOi15MjDo M3\RdYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz NEjEOGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGzN|I1Pid-Mk[xN|MzPDZ:L3G+
Jurkat  M33MZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX6xb|hHOC5zLUWwJO69VQ>? MnXSNVIuPzJiaB?= NXm5S4hEcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? Moq2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzM{OyOFYoRjJ4MUOzNlQ3RC:jPh?=
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Jurkat  MV;GeY5kfGmxbjDBd5NigQ>? M17nRVMvPcLiwsXN NXL6WZZ[PDhiaB?= MYnpcoNz\WG|ZYRCpHBVWExzwrDtVm5CKGW6cILld5Nqd25? NF71cGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGzN|I1Pid-Mk[xN|MzPDZ:L3G+
MDA-MB-231 MVXGeY5kfGmxbjDBd5NigQ>? NFPufWgyNzJwNT:1JO69VQ>? NFXpOlk1QCCq M1;3PIRm[3KnYYPld{B1cGViUGTQUlEzKGW6cILld5Nqd25iYYSgeIhmKGOxbnPldo51emG2aX;uJI9nKDYEoN88UUA1QMLiaB?= NVHYOWNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NVczOjlpPkK1PFE4OjJ7PD;hQi=>
MDA-MB-231 M3TXUmZ2dmO2aX;uJGF{e2G7 MnXxNU8zNjVxNTFOwG0> MnPuOFghcA>? NIS0VG5qdmO{ZXHz[ZMhfGinIHzleoVteyCxZjDFMYNi\GincnnuJI1TVkFiYYSgeIhmKGOxbnPldo51emG2aX;uJI9nKDJwNdMg{txO\m:{IES4xsBp NEXsd3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUixO|IzQSd-MkW4NVczOjl:L3G+
MDA-MB-231 NFzFSFNHfW6ldHnvckBCe3OjeR?= MX[xM|IvPS93IN88US=> MlnrNlQwPDhiaB?= MnXZbY5lfWOnczCgd4lodmmoaXPhcpQhWEGUUDDjcIVifmGpZTDh[pRmeiB2ODDo MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzN{KyPUc,OjV6MUeyNlk9N2F-
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BGC-823 NIDiZlRHfW6ldHnvckBCe3OjeR?= M1;scVXDqM7:TR?= MofaO|IhcA>? MYHk[YNz\WG|ZYOgeIhmKFCUTD2zJJBzd3SnaX6gcIV3\Wxic3nncoln[2GwdHz5 NWjlV3pORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0O|U4OzNpPkK1OFc2PzN|PD;hQi=>
MKN28 NE\YWnNHfW6ldHnvckBCe3OjeR?= NGrhO4I2yqEQvF2= NIrRU5M4OiCq NY\td|Rk\GWlcnXhd4V{KHSqZTDQVmwuOyCycn;0[YlvKGyndnXsJJNq\26rZnPhcpRtgQ>? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR5NUezN{c,OjV2N{W3N|M9N2F-
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MKN45 MlPWSpVv[3Srb36gRZN{[Xl? MYK1xsDPxE1? MWq3NkBp M{KzVYRm[3KnYYPld{B1cGViUGLMMVMheHKxdHXpckBt\X[nbDDzbYdvcW[lYX70cJk> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR5NUezN{c,OjV2N{W3N|M9N2F-
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HepG2  MXnGeY5kfGmxbjDBd5NigQ>? M2fnbFAuOjVizszN MVqyOEBp NVniO2d[\GWlcnXhd4V{KHO3YoTpcIl{cW5xa3X4bY4hfHmyZTC5JEhRS1ONOTmgdJJwfGWrbjDs[ZZmdHNiZH;z[UBl\XCnbnTlcpRtgQ>? NX7VW2ZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVU3PDZpPkK0PFU2PjR4PD;hQi=>
HepG2  MX\GeY5kfGmxbjDBd5NigQ>? NYK1PHpnOC1{NTFOwG0> MVGyOEBp NEPaUo5qdmO{ZXHz[ZMhdG:5IHTlcpNqfHlibHnwc5Bzd3SnaX6gdoVk\XC2b4KgLGxFVFJrIHflcoUh\XiycnXzd4lwdsLi NYS1WGM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVU3PDZpPkK0PFU2PjR4PD;hQi=>
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HepG2  NFHNcXRHfW6ldHnvckBCe3OjeR?= NXXm[5hKOTBizszt MmDzNlQhcA>? M1TZb5Bz\X[nboTzJHNTTUKSIIDyc4Nme3Orbne= MmrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NUW2OFYoRjJ2OEW1OlQ3RC:jPh?=
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CNDT2  NGHuTlNHfW6ldHnvckBCe3OjeR?= NETCOFY2yrWPwrC= NYDHUnMxPCCm M1vEV5Jm\HWlZYOgeIhmKG2ndHj5cIF1cW:wIHzleoVteyCxZjDXTWYyNCCSMU[sJGNZS0xzNDygUmtZOuLCk{OsJGNFUDFuIFzBUWEyNCCjbnSgR3RPVkJz Mnf3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5NkK4NFkoRjJ2N{[yPFA6RC:jPh?=
CNDT2  MkO3SpVv[3Srb36gRZN{[Xl? NVvIfFhkPcL3TdMg MljIOEBl NHHxUoFqdmO{ZXHz[ZMh\2WwZTDlfJBz\XO|aX;uJI9nyqCZSV[xMEBROTZuIFPETFEtyqCOQV3BNUzDqGGwZNMgR3RPVkJz NH;UTY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2NlgxQSd-MkS3OlI5ODl:L3G+
T-cells Ml23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TVN|UwOjBizszN MnPTNE01QCCq MmfObY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> M4HVb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{W3Nlg{Lz5{NEe1O|I5OzxxYU6=
CD3+ T-cells NX;KOZdwTnWwY4Tpc44hSXO|YYm= NHvOemc2NzJyIN88US=> M2HSe|Q5KGh? MlvBeZBz\We3bHH0[ZMheDF3IHX4dJJme3Orb36= NIrIUmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe1O|I5Oyd-MkS3OVczQDN:L3G+
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CD8+ T-cells MXXGeY5kfGmxbjDBd5NigQ>? MWO1M|IxKM7:TR?= NID1ZnY1QCCq NXTXemw6fXC{ZXf1cIF1\XNicEG1JIV5eHKnc4Ppc44> NEHsOIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe1O|I5Oyd-MkS3OVczQDN:L3G+
CD3+ T-cells NEK2RXNHfW6ldHnvckBCe3OjeR?= MVS1M|IxKM7:TR?= NHG0fWk1QCCq MV;1dJJm\3WuYYTld{B1cGViZYjwdoV{e2mxbjDv[kBHV1iSMx?= M2HnSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{W3Nlg{Lz5{NEe1O|I5OzxxYU6=
CD4+ T-cells NFzWW|JHfW6ldHnvckBCe3OjeR?= NEHlVnM2NzJyIN88US=> NXfSfohrPDhiaB?= MW\1dJJm\3WuYYTld{B1cGViZYjwdoV{e2mxbjDv[kBHV1iSMx?= M1LwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{W3Nlg{Lz5{NEe1O|I5OzxxYU6=
CD4+ T-cells NXO2fWxzTnWwY4Tpc44hSXO|YYm= M1:zc|UwOjBizszN MmLzOFghcA>? NUj1SVhTemWmdXPld{BVSkWWMdMgcXJPSSCneIDy[ZN{cW:w M3nSOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{W3Nlg{Lz5{NEe1O|I5OzxxYU6=
CD4+ T-cells NFfyUVZHfW6ldHnvckBCe3OjeR?= MVq1M|IxKM7:TR?= MYe0PEBp NI\XOHd2eHKnZ4XsZZRmeyC2aHWg[ZhxemW|c3nvckBw\sLiUl;S{tN1yqB? NWPibZp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OVczQDNpPkK0O|U4Ojh|PD;hQi=>
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CD8+ T-cells NXi0UGhKTnWwY4Tpc44hSXO|YYm= NEHCfVE2NzJyIN88US=> NVTVTItOPDhiaB?= MYLpcohq[mm2czDt[Y1wenliVD3j[Yxtew>? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd3N{K4N{c,OjR5NUeyPFM9N2F-
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MCF7 MWHGeY5kfGmxbjDBd5NigQ>? NGPYPIU2KM7:TR?= M4nsN|Q5KGkEoB?= MlGx[Il{eGyjeYOgd4Vt\WO2aY\lJJRwgGmlaYT5JJRwf2G{ZDDzeZNx\W6mZXSgUWNHNTdiY3XscJM> M1vrfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkOzN|UxLz5{NE[zN|M2ODxxYU6=
MCF7 M2X0WGZ2dmO2aX;uJGF{e2G7 NVLDOW5QOTBizszN NVv6[21iOjRiaNMg MUnpcoR2[2W|IITo[UBkdGWjdnHn[UBw\iClYYPwZZNmKDdiYX7kJHBCWlEEoB?= NVTYeog1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|M{PTBpPkK0OlM{OzVyPD;hQi=>
MCF7 M1LGbmZ2dmO2aX;uJGF{e2G7 NGrXdZox6oDVMD61JO69VcLi NHvWXJI4KGR? NXzwPIJRcW6qaXLpeJMhfGinIHfyc5d1cCCPQ1[tO{B1fW2xcoPwbIVz\XNiaX6gd5V{eGWwc3nvckBkfWy2dYLld:Kh MlzoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{OzOVAoRjJ2NkOzN|UxRC:jPh?=
MCF7 MlPuSpVv[3Srb36gRZN{[Xl? M3\5elAvPSEQvF5CpC=> M13iRlE1KGR? MlHTdoVlfWOnczD0bIUhe2m8ZTDv[kBOS0ZvNzDjc4xwdmmnczDlcYJm\GSnZDDpckB{d2[2IHHnZZI> MorKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{OzOVAoRjJ2NkOzN|UxRC:jPh?=
MCF7 MXHGeY5kfGmxbjDBd5NigQ>? NIGxU3AxNjB34pETNlAh|ryP Ml;VNUBl NWDRd2hMemWmdXPld{B1cGViY3zvcoFtKHO3co\peoFtKG:oIF3DSk04KGOnbHzzJIlvKG2xbn;sZZlmeiCldXz0eZJmew>? NEHVSVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zN|M2OCd-MkS2N|M{PTB:L3G+
T47D  MWTGeY5kfGmxbjDBd5NigQ>? NVf0UYhiOC53IN88US=> NWK5XFZwPCCm MWfpcohq[mm2czD0eY1wenOyaHXy[UBnd3KvYYTpc44> M1G1e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkOzN|UxLz5{NE[zN|M2ODxxYU6=
MCF7 NULhcHY5TnWwY4Tpc44hSXO|YYm= NGm5Z4QxNjYkgKOxNEDPxE1? M4LIdFQ5KGkEoB?= M1\pXIlvcGmkaYTzJJRp\SCpYYCgZ4xwe3W{ZTDpckB1cGVid3;1coQhcGWjbHnu[{Bie3OjeR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|M{O1NEc,OjR4M{OzOVA9N2F-
MCF7 MVLGeY5kfGmxbjDBd5NigQ>? M{\reVAwOTBizszN M3HEclI1KGh? NXvMZm5RcW6qaXLpeJMhfGinIHHjeIl3cXS7IH;mJG1OWDl? NXnFeXRKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|M{PTBpPkK0OlM{OzVyPD;hQi=>
MDA-MB-231  NXruS|FQTnWwY4Tpc44hSXO|YYm= MnS1NE426oDVMUCg{txO NGm5dJA{PiCq M1rOT4lvcGmkaYTzJJRp\SCvaXfyZZRqd25? NIK1XXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zN|M2OCd-MkS2N|M{PTB:L3G+
SKM1-S NEXGeYJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NFrITZg1QCCqcoO= MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONTUGtV{Bk\WyuczDh[pRmeiB2ODDodpMh[nliWGTUJIF{e2G7LDDJR|UxKD1iMD61JO69VS5? MkPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOUS5N|goRjJ6MEm0PVM5RC:jPh?=
SKM1-S MU\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NGexclQ1QCCqcoO= Mmm4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVS12xMXMh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IFTBVGkue3SjaX7pcocu[mG|ZXSg[oxwfyCleYTvcYV1emmlIH3leIhw\CxiRVO1NEA:KDBwNUGg{txONg>? M4TTcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEm0PVM5Lz5{OEC5OFk{QDxxYU6=
A427 MWTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NIKxcVU6PiCqcoO= M{D0dWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUSyO{Bk\WyuczDh[pRmeiB7NjDodpMh[nliY4L5d5RidCC4aX;s[ZQh[XO|YYmsJGlEPTBiPTCwMlY{KM7:TT6= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR|NEG2N{c,OTh2M{SxOlM9N2F-
KYSE70 M3zsdGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MY[5OkBpenN? NYjsVW9PSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLXXNGPzBiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JINzgXO2YXygeolwdGW2IHHzd4F6NCCLQ{WwJF0hOS53OTFOwG0v M2\QbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEO0NVY{Lz5zOESzOFE3OzxxYU6=
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HT-29 M3H5[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MoHWPVYhcHK| NES0fVNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCzMlgh|ryPLh?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd6NES5K|4zPzd6NES5QE9iRg>?
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MCF7 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4XoTlk3KGi{cx?= NWe3VI9LSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDjdpl{fGGuII\pc4xmfCCjc4PhfUwhUUN3MDC9JFYvPzhizszNMi=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR|NEG2N{c,OTh2M{SxOlM9N2F-
U373-MAGI Mn7BRY51cX[rcnHsJIF{e2G7 MYGyOUB1dyB2MECgeW0> NWTFWldyOiC2bzC3NkBpenN? M4DyN2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZUXi2JIIDz[ZVld3S7cHXkJGhKXi1zIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKFV|N{OtUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[4FoKGyndnXsJIF1KDJ3IITvJFQxOCC3TTDh[pRmeiB{IITvJFczKGi{czDifUByWEOUIH3leIhw\A>? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI M1HYeWFvfGm4aYLhcEBie3OjeR?= MYWyOUB1dyB2MECgeW0> NEHrVVkzKHSxIEeyJIhzew>? NEHrW3dCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBXW1ZvRzDwd4V2\G:2eYDl[EBJUVZvMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJHU2NWejZzDs[ZZmdCCjdDCyOUB1dyB2MECgeW0h[W[2ZYKgNkB1dyB5MjDodpMh[nlicWDDVkBu\XSqb3S= Mn7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
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TC32 MlHhdWhVWyCjc4PhfS=> M4To[5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| M4\rPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 NVzzXGJpeUiWUzDhd5NigQ>? NVf3PYJmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= NGficpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-37 MVHxTHRUKGG|c3H5 NUfkR5VbeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz M3y4eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD NV7JcW9[eUiWUzDhd5NigQ>? M17jOJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? NWTiSGNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-12 MoLPdWhVWyCjc4PhfS=> NHHQ[mRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 NX:xTnRbeUiWUzDhd5NigQ>? MnTGdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= MmDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 MmTHdWhVWyCjc4PhfS=> M2LDcJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= M4jiW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC NUDxTZB5eUiWUzDhd5NigQ>? NIHqTGFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> Mnn0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SKM1-S Mn3HRZBweHSxc3nzJIF{e2G7 MlnoNUB2VQ>? NXPl[|hUUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDTT20yNVNiY3XscJMh[XO|ZYPz[YQh[XNiY3HzdIF{\SB|IHPs[YF3[WenIHH0JFEhfU1iYomgW4V{fGW{bjDicI91KG2ndHjv[C=> Mof2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOUS5N|goRjJ6MEm0PVM5RC:jPh?=
MCF7 M{LoNmZ2dmO2aX;uJIF{e2G7 MljHNVUhfU1? Mn\0O|IhcHK| NHLLXplKdmirYnn0bY9vKG:oIGXIVmYyKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hdWW2aInsZZRqd25iYYSgVmFTKGKndHGg[ZhwdiCjdDCxOUB2VSCjZoTldkA4OiCqcoOgZpkhdWW2aInsZZRqd25ic4DlZ4lncWNvUFPSJI1mfGixZB?= NHS3eIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C0PVU4Pyd-MkewOFk2Pzd:L3G+

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western blot
DNMT1 ; 

PubMed: 28210112     


AZA and DAC cause DNMT1 depletion in NSCLC cell lines. A549 and H1299 cells were treated with AZA or DAC (0–5 µM) for 20 hours and DNMT1 protein was detected by Western blotting of cell extracts. Alpha-tubulin was used as a loading control.

c-PARP / p-H2AX / H2AX; 

PubMed: 28210112     


AZA, but not DAC, induces markers of DNA damage and apoptosis in NSCLC cell lines. A549 and H1299 cells were treated with AZA or DAC (0–10 µM) for 48 hours and Western blotting of cell extracts was used to detect DNMT1, cleaved-PARP, phospho-histone-H2AX(䲧疝Ỵ疞㧀疜膉痘瘿뙠ෆᾰƌෆĀ㺣痖帉痖Ѐ瑖堘

28210112
Immunofluorescence
HMGB1 ; 

PubMed: 29097772     


U2OS cells stably expressing a GFP-HMGB1 fusion were maintained in control conditions (Ctrl), suberoylanilide hydroxamic acid (SAHA), azacitidine (AZA) for 24 h or 48 h, followed by assessment of cytoplasmic GFP-HMGB1 fluorescence intensity (A,B) or endog䲧疝Ỵ疞㧀疜膉痘瘿뙠ෆᾰƌෆĀ㺣痖帉痖Ѐ瑖堘𢡄빢᎒ෆĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ⟸෕䐺痖暼瘿⟸෕ᾰƌ ⟸෕Ð㺣痖⟸෕€𢡄⟼෕€䀷痗⟼෕౴⟼෕㵶痗⟼෕뺖᎒泌Itemｾ᎒

29097772
Growth inhibition assay
Cell viability ; 

PubMed: 28210112     


AZA and DAC differentially affect cell viability in a panel of NSCLC cell lines. Viability of A549, H460, and H1299 cells was assessed after 72 hours of treatment with AZA or DAC (0–25 µM). Error bars represent the standard error of mean of 3 independent 䲧疝Ỵ疞㧀疜膉痘瘿뙠ෆᾰƌෆĀ㺣痖

28210112
体内試験 Azacitidine inhibits polynucleotide synthesis in leukemic BDF1 mice. [3] Azacitidine (3 mg/kg, i.p.) increases the mean survival time in leukemic BDF1 mice inoculated with Ll210 ascites tumor cells. Azacitidine markedly suppresses all enzymes activity in the polyamine-biosynthetic pathway, including ornithine decarboxylase activity. putrescine-dependent S-adenosyl-L-methionine decarboxylase activity, and spermidine-dependent S-adenosyl-L-methionine decarboxylase activity. Azacitidine also inhibits the accumulations of polyamines in leukemic mice. [4]

お薦めの試験操作（参考用のみ）

細胞試験： [3]
- 合併
  • 細胞株： Leukemia L1210 cell
  • 濃度： 0.15 μg/mL
  • 反応時間： 3 days
  • 実験の流れ： 5 mL of L1210 cells (5 × 103 cells/mL) are incubated with Azacitidine at 37 ℃ for 3 days. Cell number is determined twice a day for 3 days by means of a Model A Coulter counter.
    (参考用のみ)
動物試験：[4]
- 合併
  • 動物モデル： BDF1 mice bearing lymphoid leukemia L1210
  • 投薬量： 3 mg/kg
  • 投与方法： Daily i.p. injection
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 48 mg/mL (196.56 mM) warming
Water Insoluble
Ethanol Insoluble
体内 左から（NMPから）右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5% DMSO+30% PEG 300+ddH2O
混合させたのち直ちに使用することを推奨します。 		10mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 244.2
化学式

C8H12N4O5
CAS No. 320-67-2
Storage powder
in solvent
別名 5-AzaC,Ladakamycin, AZA,5-Aza, CC-486
Smiles C1=NC(=NC(=O)N1C2C(C(C(O2)CO)O)O)N

投与溶媒組成計算器(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）
投与量 mg/kg 動物平均体重 g 投与体積（動物毎） ul 動物数 匹
ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとSDS/COA（製品ページで利用可能な）。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます：

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます：C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとSDS/COA（オンラインで利用できる）で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください：

総分子量：g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05155709 Not yet recruiting Drug: siremadlin|Drug: venetoclax|Drug: azacitidine Acute Myeloid Leukemia Novartis Pharmaceuticals|Novartis February 24 2022 Phase 1|Phase 2
NCT05140811 Not yet recruiting Drug: IMM01|Drug: Azacitidine Acute Myeloid Leukemia|Myelodysplastic Syndromes ImmuneOnco Biopharmaceuticals (Shanghai) Co. Ltd|Institute of Hematology & Blood Diseases Hospital January 1 2022 Phase 1|Phase 2
NCT05141682 Not yet recruiting Drug: Oral Azacitidine Recurrent T-Cell Large Granular Lymphocyte Leukemia|Refractory T-Cell Large Granular Lymphocyte Leukemia|T-Cell Large Granular Lymphocyte Leukemia Jonathan Brammer|Bristol-Myers Squibb|Ohio State University Comprehensive Cancer Center December 1 2021 Phase 1|Phase 2
NCT05074355 Not yet recruiting Drug: Azacitidine|Drug: Venetoclax Myeloproliferative Neoplasm|BCR-ABL Negative University Health Network Toronto December 6 2021 Phase 2
NCT04891068 Recruiting Drug: Azacitidine Breast Cancer Female|Breast Cancer Invasive University of Illinois at Chicago December 31 2021 Phase 2

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問（FAQ）

  • 質問1：

    Is the vehicle (30% Propylene glycol, 5% Tween 80, 65% D5W) for Azacitidine (Catalog No.S1782) safe for subcutaneous dosing?

  • 回答：

    S1782 in 30% Propylene glycol+5% Tween 80+65% D5W at 30mg/ml is a suspension. If you are going to administrate this compound for oral gavage, it is fine. But if you administrate the drug via injection, you need a clear solution and S1782 can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 10mg/ml clearly.

selleck catalog

DNA Methyltransferaseシグナル伝達経路
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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID