Etoposide

For research use only. Not for use in humans.

製品コードS1225 別名:VP-16, VP-16213

Etoposide化学構造

CAS No. 33419-42-0

Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.

サイズ 価格(税別) 在庫  
10mM (1mL in DMSO) JPY 17100 あり
JPY 13600 あり
JPY 48400 あり
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バルク問合せ

文献中Selleckの製品使用例(160)

製品安全説明書

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

生物活性

製品説明 Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.
ターゲット
Topo II [2]
(Cell-free assay)
体外試験

Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA, which induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle. [1] Etoposide inhibits the growth of murine angiosarcoma cell line (ISOS-1) in a 5 days-period with IC50 of 0.25 μg/mL. Cell growth of normal murine microvascular endothelial cells (mECs) is less sensitive to Etoposide with IC50 of 10 μg/mL). [2] Etoposide treated for 6 hr inhibits colonies of tetraploid variant of the human leukemic lymphoblast line CCRF-CEM with IC50 of 0.6 μM. [3] Etoposide treated for 2 hr inhibits growth of human pancreatic cancer cell line Y1, Y3, Y5, Y19, YM. YS, and YT with IC50s of 300 μg/mL, 300 μg/mL, 300 μg/mL, 91 μg/mL, 0.68 μg/mL, 300 μg/mL, 300 μg/mL, and 260 μg/mL, respectively. [4] Etoposide exposed for 1 hr inhibits growth of human glioma cell lines CL5, G142, G152, G111, and G5 with IC50 of 8, 9, 9.8, 10, and 15.8 μg/mL respectively for 12 days. Under same condition, the IC90 value is attained in cell lines CL5, G152, G142, and G111 at 26, 27, 32, and 33 μg/mL. Etoposide inhibition of topoisomerase II is homogeneous for each cell. The average inhibition rates are 15%, 21.8%, 31.8%, 41.5%, and 49.5% for 1, 2, 4, 8, and 16 μg Etoposide, respectively. [5]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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U-266 NGnTVIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mom0NlQhcA>? NF3vU29KSzVyPUi2MlLDqM7:TR?= M1nROFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MES4NlM3Lz5{NUC0PFI{PjxxYU6=
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Kelly NHvCdG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfBTow1OC1zMDFOwG0> MYS3NuKhcA>? M{fGNWlEPTB;MT61NVjDqM7:TR?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOEmwNEc,OjVyMEi5NFA9N2F-
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HL-60  NIS3W2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXWO|LDqGh? NYGwU2ZNUUN3ME2wMlEz6oDHzszN NHLsO489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5N|AyPCd-MkS5PVMxOTR:L3G+
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MO59K  MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDqO{Bl NHTXSVdKSzVyPUCuNVfjiIYQvF2= M2TreFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUWzOVYyLz5{NEm1N|U3OTxxYU6=
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HL60/MX2 M1fPSmN6fG:2b4jpZ4l1gSCjc4PhfS=> MXO3NkBpenN? NE\5XoFEgXSxdH;4bYNqfHliYXfhbY5{fCCvaYTvfIFvfHKxbnWtdoV{cXO2YX70JIh2dWGwIFjMOlAwVVh{IHPlcIx{KGGodHXyJFczKGi{czygTWM2OCB;IES4MlUh|ryPLh?= NELOUXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEWwO|M3QCd-MUi1NFc{Pjh:L3G+
HL60 MorSR5l1d3SxeHnjbZR6KGG|c3H5 NVzmRpFSPzJiaILz MmrIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= Mn7zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MEe0O|IoRjF6NUC3OFczRC:jPh?=
HL60 NVXFOZoxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEKwdIY1QCCqcoO= MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwM{G1JO69VS5? NYfrUZZ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVM6PzRpPkG4OVE{QTd2PD;hQi=>
K562 MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MXW0PEBpenN? MmCyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNNU[yJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkK3JO69VS5? NVj1fHJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVQ6PzJpPkG4OVE1QTd{PD;hQi=>
KB MV\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXriU3hTPDhiaILz NH\RdZRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFvCJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkSyJO69VS5? M{nISlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUG0PVczLz5zOEWxOFk4OjxxYU6=
L02 NVH3SGR4SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M2rkZVQ5KGi{cx?= NEThTo9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFywNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMUOg{txONg>? NXnsRnBFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVQ6PzJpPkG4OVE1QTd{PD;hQi=>
DU145 ATCC HTB 81 MnHQR5l1d3SxeHnjbZR6KGG|c3H5 MlfzO|IhcHK| NHP1S4tEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDBWGNEKEiWQjC4NUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT61JO69VS5? NUL5TJllRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OVg1QTBpPkG4OVU5PDlyPD;hQi=>
K562 ATCC CCL 243 MkDoR5l1d3SxeHnjbZR6KGG|c3H5 MmXrO|IhcHK| NHT3WWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IFHUR2MhS0OOIEK0N{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iND65JO69VS5? M{HJUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
MCF7 ATCC HTB 22 M1;nPWN6fG:2b4jpZ4l1gSCjc4PhfS=> NFHZW2s4OiCqcoO= MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KEGWQ1OgTHRDKDJ{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyOi5{IN88UU4> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3OES5NEc,OTh3NUi0PVA9N2F-
U251 RCB 0641 NXy1W5NwS3m2b4TvfIlkcXS7IHHzd4F6 NFLOT4o4OiCqcoO= NELjd3VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWOjVzIGLDRkAxPjRzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjVizszNMi=> M{fHW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
3LL CRL 1642 Mn3KR5l1d3SxeHnjbZR6KGG|c3H5 M{HGPVQ5KGi{cx?= MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTCzUGwhS1KOIEG2OFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IESg{txONg>? M2joN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
L1210 ATCC CCL 219 NXPvWVJzS3m2b4TvfIlkcXS7IHHzd4F6 NITpemg1QCCqcoO= MmjyR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFEzOTBiQWTDR{BES0xiMkG5JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkWg{txONg>? NY\pNI5bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OVg1QTBpPkG4OVU5PDlyPD;hQi=>
SK-Hep1 NVPqNHM3S3m2b4TvfIlkcXS7IHHzd4F6 NFHKdWFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2KZYCxJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQ6NjZizszNMi=> NWL3U4ZzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2N|c3QDhpPkG4OlM4Pjh6PD;hQi=>
HL60 MWnDfZRwfG:6aXPpeJkh[XO|YYm= MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwMjFOwG0v M3To[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkWxO|cyLz5zOE[1NVc4OTxxYU6=
A549 M{LremN6fG:2b4jpZ4l1gSCjc4PhfS=> MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCxZjDj[YxtKGe{b4f0bEBjgSCVUlKgcYV1cG:mLDDJR|UxKD1iOT65PEDPxE1w Mmq0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5OEK2OlgoRjF6N{iyOlY5RC:jPh?=
KB M3nKPWN6fG:2b4jpZ4l1gSCjc4PhfS=> NYPub|FZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IGPSRkBu\XSqb3SsJGlEPTBiPTCxOE4zKM7:TT6= MoTmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5OEK2OlgoRjF6N{iyOlY5RC:jPh?=
A549 MoTTR5l1d3SxeHnjbZR6KGG|c3H5 NUHhOY82PzJiaILz M4TvV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFgvOzdizszNMi=> NHLw[mY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEi0OFQzOid-MUi4OFQ1OjJ:L3G+
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AGS NVOwZZV4S3m2b4TvfIlkcXS7IHHzd4F6 Ml\jN{Bl[Xm| M3y3OmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFIWyClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4{PiEQvF2u M4f2eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEGzNFc1Lz5zOUCxN|A4PDxxYU6=
HL-60 NF74W|VEgXSxdH;4bYNqfHliYYPzZZk> MWWzJIRigXN? MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUE03OCClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE45KM7:TT6= MnrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
J82 MoD5R5l1d3SxeHnjbZR6KGG|c3H5 M4rZRVMh\GG7cx?= M2T0cmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGo5OiClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk45KM7:TT6= NHPUeZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCxN|A4PCd-MUmwNVMxPzR:L3G+
MRC5 M4fRPGN6fG:2b4jpZ4l1gSCjc4PhfS=> MYqzJIRigXN? NGD2UWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUA{NjlizszNMi=> NXyw[FJsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNVMxPzRpPkG5NFE{ODd2PD;hQi=>
SK-MES-1 NGP1[49EgXSxdH;4bYNqfHliYYPzZZk> NID6W24{KGSjeYO= M1myRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNU2HUz2xJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{LkWg{txONg>? NXPhXI9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNVMxPzRpPkG5NFE{ODd2PD;hQi=>
A375 NXjjbpB6S3m2b4TvfIlkcXS7IHHzd4F6 M1;nbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMz65NkDPxE1w NY\mVoJERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwN|Y2QDRpPkG5NFM3PTh2PD;hQi=>
A549 M17XdGN6fG:2b4jpZ4l1gSCjc4PhfS=> M1zYbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iOT61NUDPxE1w M4rxRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEO2OVg1Lz5zOUCzOlU5PDxxYU6=
HL60 M2jHfmN6fG:2b4jpZ4l1gSCjc4PhfS=> NV2wSpZSS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlg{KM7:TT6= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB|NkW4OEc,OTlyM{[1PFQ9N2F-
THP1 MXLDfZRwfG:6aXPpeJkh[XO|YYm= M4\BeGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHRJWDFiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj6xOkDPxE1w M2rseVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEO2OVg1Lz5zOUCzOlU5PDxxYU6=
A549 NIrnZlBEgXSxdH;4bYNqfHliYYPzZZk> NFPCOYQ4OiCqcoO= M{n6TWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFUvQSEQvF2u MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{WxOEc,OTlyNUO1NVQ9N2F-
HL-60 M{TwdGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mn7SO|IhcHK| MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUE03OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4zPSEQvF2u MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{WxOEc,OTlyNUO1NVQ9N2F-
U937 MXnDfZRwfG:6aXPpeJkh[XO|YYm= NUnwWGRZPzJiaILz NFHGeZREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjl{IN88UU4> M4XYNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzOVE1Lz5zOUC1N|UyPDxxYU6=
ACHN M2LYWmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M2fX[Vk3KGi{cx?= MonsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDQ1jOJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{LkG1JO69VS5? M2P0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO|Y4Lz5zOUC1N|c3PzxxYU6=
CCRF-CEM NY\DfHJMSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M2T4Slk3KGi{cx?= MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEOFUl[tR2VOKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlIh|ryPLh?= NFnVUlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC1N|c3Pyd-MUmwOVM4Pjd:L3G+
CCRF-SB MXHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NHX4RWY6PiCqcoO= MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEOFUl[tV2Ih[2WubIOgZYZ1\XJiOU[gbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNUDPxE1w NYPLSYFnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwOVM4PjdpPkG5NFU{PzZ5PD;hQi=>
HeLa MVrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M3TYWVk3KGi{cx?= NVnJepMxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCzJO69VS5? NVP3XYRrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwOVM4PjdpPkG5NFU{PzZ5PD;hQi=>
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