Etoposide (VP-16)

For research use only. Not for use in humans.

製品コードS1225 別名:VP-16213

Etoposide (VP-16)化学構造

CAS No. 33419-42-0

Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.

サイズ 価格(税別)  
10mM (1mL in DMSO) JPY 17100
JPY 13600
JPY 48400
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文献中Selleckの製品使用例(220)

製品安全説明書

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

生物活性

製品説明 Etoposide (VP-16, VP-16213) is a semisynthetic derivative of podophyllotoxin, which inhibits DNA synthesis via topoisomerase II inhibition activity. Etoposide induces autophagy, mitophagy and apoptosis.
ターゲット
Topo II [2]
(Cell-free assay)
体外試験

Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA, which induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle. [1] Etoposide inhibits the growth of murine angiosarcoma cell line (ISOS-1) in a 5 days-period with IC50 of 0.25 μg/mL. Cell growth of normal murine microvascular endothelial cells (mECs) is less sensitive to Etoposide with IC50 of 10 μg/mL). [2] Etoposide treated for 6 hr inhibits colonies of tetraploid variant of the human leukemic lymphoblast line CCRF-CEM with IC50 of 0.6 μM. [3] Etoposide treated for 2 hr inhibits growth of human pancreatic cancer cell line Y1, Y3, Y5, Y19, YM. YS, and YT with IC50s of 300 μg/mL, 300 μg/mL, 300 μg/mL, 91 μg/mL, 0.68 μg/mL, 300 μg/mL, 300 μg/mL, and 260 μg/mL, respectively. [4] Etoposide exposed for 1 hr inhibits growth of human glioma cell lines CL5, G142, G152, G111, and G5 with IC50 of 8, 9, 9.8, 10, and 15.8 μg/mL respectively for 12 days. Under same condition, the IC90 value is attained in cell lines CL5, G152, G142, and G111 at 26, 27, 32, and 33 μg/mL. Etoposide inhibition of topoisomerase II is homogeneous for each cell. The average inhibition rates are 15%, 21.8%, 31.8%, 41.5%, and 49.5% for 1, 2, 4, 8, and 16 μg Etoposide, respectively. [5]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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KellyCis83 NGDveolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlT4TWM2OD1yLkG25qCKyrIkgJmwMlAzKM7:TR?= M2O4W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OU[wNlgzLz5{NUm2NFI5OjxxYU6=
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HL60 M3XoUWN6fG:2b4jpZ4l1gSCjc4PhfS=> M1ntTlczKGi{cx?= NXzFVI84S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdWm2b4jhcpRzd26nLYPlcpNqfGm4ZTDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyxiSVO1NEA:KDBwNEi2JO69VS5? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDVyN{O2PEc,OTh3MEezOlg9N2F-
HL60/MX2 MkjRR5l1d3SxeHnjbZR6KGG|c3H5 NEXweHc4OiCqcoO= MknjR5l1d3SxeHnjbZR6KGGpYXnud5QhdWm2b4jhcpRzd26nLYLld4l{fGGwdDDoeY1idiCKTE[wM21ZOiClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVAhRSB2OD61JO69VS5? M{\w[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUC3N|Y5Lz5zOEWwO|M3QDxxYU6=
HL60 NYjHNIpKS3m2b4TvfIlkcXS7IHHzd4F6 NUmxVFhxPzJiaILz NVrDSotES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{IN88UU4> Moi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MEe0O|IoRjF6NUC3OFczRC:jPh?=
HL60 MoDVRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M4nQT|Q5KGi{cx?= M37EcGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFy2NEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6zNVUh|ryPLh?= NWfjeWRbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVM6PzRpPkG4OVE{QTd2PD;hQi=>
K562 NXHJXmJDSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MnO3OFghcHK| M2DmZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iS{W2NkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT6yO{DPxE1w MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDVzNEm3Nkc,OTh3MUS5O|I9N2F-
KB NITiRVhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MkW4OFghcHK| NH3HclNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFvCJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkSyJO69VS5? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDVzNEm3Nkc,OTh3MUS5O|I9N2F-
L02 MUTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYHyNYhEPDhiaILz NVXzWIR5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMNFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGzJO69VS5? Mni3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MUS5O|IoRjF6NUG0PVczRC:jPh?=
DU145 ATCC HTB 81 MmDVR5l1d3SxeHnjbZR6KGG|c3H5 MkO2O|IhcHK| MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSCDVFPDJGhVSiB6MTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOS53IN88UU4> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV3OES5NEc,OTh3NUi0PVA9N2F-
K562 ATCC CCL 243 NGjNe5BEgXSxdH;4bYNqfHliYYPzZZk> NW\a[W9EPzJiaILz NFK4e|ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IFHUR2MhS0OOIEK0N{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iND65JO69VS5? MmW4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
MCF7 ATCC HTB 22 M{XWUGN6fG:2b4jpZ4l1gSCjc4PhfS=> Ml\0O|IhcHK| M4nsSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiQWTDR{BJXEJiMkKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF{LkKg{txONg>? MmrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
U251 RCB 0641 NWPxW3VWS3m2b4TvfIlkcXS7IHHzd4F6 NXrMdGpPPzJiaILz M1\PbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHUzPTFiUlPCJFA3PDFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvPSEQvF2u MmWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi0PVAoRjF6NUW4OFkxRC:jPh?=
3LL CRL 1642 NXXOSWhLS3m2b4TvfIlkcXS7IHHzd4F6 MXi0PEBpenN? NHLuVJNEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UA{VExiQ2LMJFE3PDJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQh|ryPLh?= NF:zR|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW1PFQ6OCd-MUi1OVg1QTB:L3G+
L1210 ATCC CCL 219 MljCR5l1d3SxeHnjbZR6KGG|c3H5 NGfyNI81QCCqcoO= MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMNVIyOCCDVFPDJGNEVCB{MUmgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwNTFOwG0v M1m4elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OFkxLz5zOEW1PFQ6ODxxYU6=
SK-Hep1 MV3DfZRwfG:6aXPpeJkh[XO|YYm= M4fNfGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNUincEGgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOFkvPiEQvF2u NWL0bW13RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2N|c3QDhpPkG4OlM4Pjh6PD;hQi=>
HL60 NH[5emtEgXSxdH;4bYNqfHliYYPzZZk> NEXr[5pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGK7IGPSRkBie3OjeTygTWM2OCB;IECuNkDPxE1w Mnm5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NUG3O|EoRjF6NkWxO|cyRC:jPh?=
A549 NFGy[YREgXSxdH;4bYNqfHliYYPzZZk> MkLiR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gZ4VtdCCpcn;3eIgh[nliU2LCJI1mfGixZDygTWM2OCB;IEmuPVgh|ryPLh?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd6Mk[2PEc,OTh5OEK2Olg9N2F-
KB NXvLS49IS3m2b4TvfIlkcXS7IHHzd4F6 MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiY3XscEBoem:5dHigZpkhW1KEIH3leIhw\CxiSVO1NEA:KDF2LkKg{txONg>? NHz2VYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEe4NlY3QCd-MUi3PFI3Pjh:L3G+
A549 NH3DR4ZEgXSxdH;4bYNqfHliYYPzZZk> Ml3MO|IhcHK| NWjKN2VsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hQC5|NzFOwG0v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh2NESyNkc,OTh6NES0NlI9N2F-
KB NGLPW5NEgXSxdH;4bYNqfHliYYPzZZk> MUS3NkBpenN? MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT6yOUDPxE1w M2q4e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OES0OFIzLz5zOEi0OFQzOjxxYU6=
AGS NUTSV2J6S3m2b4TvfIlkcXS7IHHzd4F6 NV\te|FWOyCmYYnz MmXOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRWdUKGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlM3KM7:TT6= MmfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
HL-60 NH\we25EgXSxdH;4bYNqfHliYYPzZZk> MVOzJIRigXN? M2HSWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNNTZyIHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjhizszNMi=> M4fURlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEGzNFc1Lz5zOUCxN|A4PDxxYU6=
J82 M1qxUGN6fG:2b4jpZ4l1gSCjc4PhfS=> M3fr[|Mh\GG7cx?= NX6zdY9{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUjh{IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjhizszNMi=> MoGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMUOwO|QoRjF7MEGzNFc1RC:jPh?=
MRC5 NFP2RldEgXSxdH;4bYNqfHliYYPzZZk> NXfwOnhyOyCmYYnz MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNVmM2KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCzMlkh|ryPLh?= NHPWW5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCxN|A4PCd-MUmwNVMxPzR:L3G+
SK-MES-1 MXnDfZRwfG:6aXPpeJkh[XO|YYm= NIH0NVE{KGSjeYO= NUnUWYkxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXTMVEh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKuOUDPxE1w NF;M[mQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCxN|A4PCd-MUmwNVMxPzR:L3G+
A375 NGm2bI9EgXSxdH;4bYNqfHliYYPzZZk> M3P6PWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMz65NkDPxE1w MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB|NkW4OEc,OTlyM{[1PFQ9N2F-
A549 MmP2R5l1d3SxeHnjbZR6KGG|c3H5 M3LHO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxKD1iOT61NUDPxE1w NHPTR5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCzOlU5PCd-MUmwN|Y2QDR:L3G+
HL60 NVrTNZRjS3m2b4TvfIlkcXS7IHHzd4F6 MmPsR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkizJO69VS5? M3Hsd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEO2OVg1Lz5zOUCzOlU5PDxxYU6=
THP1 M1zHcGN6fG:2b4jpZ4l1gSCjc4PhfS=> NFH6e49EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVUFBzIHPlcIx{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKuNVYh|ryPLh?= MnX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyM{[1PFQoRjF7MEO2OVg1RC:jPh?=
A549 M4L6RmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkjkO|IhcHK| NUCzPFlHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPS57IN88UU4> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{WxOEc,OTlyNUO1NVQ9N2F-
HL-60 M4DXN2N6fG:2b4jpZ4l1gSCjc4PhfS=> NITBd3U4OiCqcoO= M{PLOWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNNTZyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ3IN88UU4> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{WxOEc,OTlyNUO1NVQ9N2F-
U937 NV3VbI9YS3m2b4TvfIlkcXS7IHHzd4F6 MV:3NkBpenN? NVrNeWZtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTl|NzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC57MjFOwG0v NYPZNJpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwOVM2OTRpPkG5NFU{PTF2PD;hQi=>
ACHN Mk\qRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NX;ieY9WQTZiaILz MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEGFSF6gZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDJwMUWg{txONg>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
CCRF-CEM MoHWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NWTGT4hRQTZiaILz NUG5[mMzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDR3JHNUOHTTDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOS5{IN88UU4> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2O{c,OTlyNUO3Olc9N2F-
CCRF-SB NUO2WFRiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NX7qXndCQTZiaILz NGLLbohCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPDVmYuW0JiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOSEQvF2u M1mzS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO|Y4Lz5zOUC1N|c3PzxxYU6=
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Hep2 NHzVbVVCdnSrY3HuZ4VzKGG|c3H5 NH3N[Zg1QCCqcoO= M3;lUmFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZAzKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCwMlkh|ryPLh?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB5NES0OEc,OjFyN{S0OFQ9N2F-
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