Irinotecan HCl Trihydrate

For research use only. Not for use in humans.

製品コードS2217 別名:CPT-11 HCl Trihydrate

Irinotecan HCl Trihydrate化学構造

分子量(MW):677.18

Irinotecan prevents DNA from unwinding by inhibition of topoisomerase 1.

サイズ 価格(税別) 在庫  
10mM (1mL in DMSO) JPY 33500 あり
JPY 13600 あり
JPY 36800 あり
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バルク問合せ

文献中Selleckの製品使用例(39)

製品安全説明書

Topoisomerase阻害剤の選択性比較

生物活性

製品説明 Irinotecan prevents DNA from unwinding by inhibition of topoisomerase 1.
特性 Irinotecan is a prodrug that is used to treat metastatic colorectal cancer.
ターゲット
Topo I [1]
体外試験

Irinotecan is activated to SN-38 by carboxylesterases to become able to interact with its target, topoisomerase I. Irinotecan induces similar amounts of cleavable complexes at its IC50 in LoVo cells and HT-29 cell lines. SN-38 induces a concentration-dependent formation of cleavable complexes, which is not significantly different in LoVo cells and HT-29 cell lines. Cell accumulation of Irinotecan is markedly different, reaching consistently higher levels in HT-29 cells than in LoVo cells. [1] The lactone E-ring of Irinotecan and SN-38 hydrolyses reversibly in aqueous solutions, and the interconversion between the lactone and carboxylate forms is dependent on pH and temperature. Liver is primarily responsible for the activation of Irinotecan to SN-38. At equal concentrations of Irinotecan and SN-38 glucuronide, the rate of beta-glucuronidase-mediated SN-38 production is higher than that formed from Irinotecan in both tumour and normal tissue. [2] Irinotecan is also converted to SN-38 in intestines, plasma and tumor tissues. [3] Irinotecan is significantly more active in SCLC than in NSCLC cell lines, whereas no significant difference between histological types is observed with SN-38. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse bone marrow cell MYnDfZRwfG:6aXPpeJkh[XO|YYm= M4jGcWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJIJwdmVibXHydo94KGOnbHygZpkhS0[XLVfNJIF{e2G7LjDJR|UxRTBwMECxJO69VQ>? Mm[zNlE{PDF4N{S=
PC3 cells NFXuSmpEgXSxdH;4bYNqfHliYYPzZZk> NVnnN4htPzJiaB?= M33aXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmuJGlEPTB;MD6wOEDPxE1? M2S2V|IyOzRzNke0
A375 cells Moq5R5l1d3SxeHnjbZR6KGG|c3H5 M1vzZVczKGh? M1;MTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MkBKSzVyPUCuNFQh|ryP MUSyNVM1OTZ5NB?=
LNCAP cells M4LLbmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkDGO|IhcA>? M1fnR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfU4hUUN3ME2wMlA6KM7:TR?= MUOyNVM1OTZ5NB?=
MESSA cells NILucIlEgXSxdH;4bYNqfHliYYPzZZk> NV[2NZloPzJiaB?= NHL0U|ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVOVQTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZkvKEmFNUC9NE4xOSEQvF2= NXHVW5JvOjF|NEG2O|Q>
H460 cells NIruToJEgXSxdH;4bYNqfHliYYPzZZk> MlXLO|IhcA>? NWK5UJg2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDR4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZkvKEmFNUC9NE4xOTVizszN Ml;nNlE{PDF4N{S=
MES-SA/Dx5 cells M13TNWN6fG:2b4jpZ4l1gSCjc4PhfS=> NVKxbIVGPzJiaB?= MoLjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWVUNVODL1T4OUBk\WyuczDveoVz\XiycnXzd4lv\yCPRGKxJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeT6gTWM2OD1yLkCyNkDPxE1? M2PmT|IyOzRzNke0
H69 cells NX\kOHRMS3m2b4TvfIlkcXS7IHHzd4F6 NW\5[3p[PzJiaB?= NXPNSIFMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDZ7IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfU4hUUN3ME2wMlAzOiEQvF2= M3rSc|IyOzRzNke0
IGROV1 cells NGPabVZEgXSxdH;4bYNqfHliYYPzZZk> MYe3NkBp MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDJS3JQXjFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MkBKSzVyPUCuNFMh|ryP M3rOVVIyOzRzNke0
SK-MEL-2 cells NXvTe4N5S3m2b4TvfIlkcXS7IHHzd4F6 MnLtO|IhcA>? NWLVXlc1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LjDJR|UxRTBwMTFOwG0> NHi2TpUzOTN2MU[3OC=>
MALME-3M cells NILibY5EgXSxdH;4bYNqfHliYYPzZZk> MVi3NkBp MoXGR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWFNVUVvM12gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NiCLQ{WwQVAvOiEQvF2= M{LQSFIyOzRzNke0
DU145 cells NV7HWIVMS3m2b4TvfIlkcXS7IHHzd4F6 MXW3NkBp NUi0bWc6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NiCLQ{WwQVAvOiEQvF2= Moe3NlE{PDF4N{S=
HT-29 cells NH3YdHREgXSxdH;4bYNqfHliYYPzZZk> MVq3NkBp Mn:4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MkBKSzVyPUCuNlIh|ryP MYiyNVM1OTZ5NB?=
H69AR cells MVvDfZRwfG:6aXPpeJkh[XO|YYm= MkXpO|IhcA>? MYXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIOllCWiClZXzsd{BwfmW{ZYjwdoV{e2mwZzDNSHIyKGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfU4hUUN3ME2wMlI2KM7:TR?= MVOyNVM1OTZ5NB?=
MCF7 cells M4PERWN6fG:2b4jpZ4l1gSCjc4PhfS=> MUOyOEBp Mnz1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhOjRiaILzJIJ6KE2WUzDhd5NigS5iSVO1NF0xNjVizszN NXO5fHh[OjZ6NEGxOlg>
HCT116 M37uRmZ2dmO2aX;uJIF{e2G7 MUnDc41xd3WwZDD3ZZMhfGW|dHXkJIlvKH[rdILvJIZweiCleYTveI95cWOrdImgZYdicW6|dDDIR3QyOTZuIHj1cYFvKGOxbH;uJINidmOncjDj[YxteyBqdHH4c4wuemW|aYP0ZY51MSCjdDDhJIRzfWdiY3;uZ4VvfHKjdHnvckBxem:mdXPpcochPTBnIHnubIljcXSrb36gc4Yh[2:ub375JIZwem2jdHnvck4hUUN3ME2wMlU1KM7:TR?= NVm3Z3dKOTF7NkWzOlI>
RPMI8402 MVzDfZRwfG:6aXPpeJkh[XO|YYm= NInteog1KGSjeYO= MorVR5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5ibIntdIhw[myjc4SgeJVud3JiY3XscEBtcW6nIGLQUWk5PDB{IHHmeIVzKDRiZHH5d{Bw\iC2cnXheI1mdnRwIFnDOVA:OC53NzFOwG0> NIHxSpEyOjd2N{e5PC=>
KB3-1 cells NXXQVIZRS3m2b4TvfIlkcXS7IHHzd4F6 MnXQR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2I{NTFiY3XscJMh[nliTWTUJI1mfGixZD6gTWM2OD1yLk[4JO69VQ>? MoTJNVg4PzF7M{C=
PC3 cells NFq0fFNRem:uaX\ldoF1cW:wIHHzd4F6 NHmweIw4OiCq NEX6Zo9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDhd5Nme3OnZDDhd{Bk\WyudXzhdkBFVkFiY3;ueIVvfCCjZoTldkA4OiCqcoOgZpkhS3mTVVHOWEBPTiCobIXvdoV{[2WwY3WgZZN{[XlwIFnDOVA:OC56IN88US=> MV[yOlc{OTNyMB?=
A549 cells NGLseINEgXSxdH;4bYNqfHliYYPzZZk> MXeyOEBp MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkAzPCCqcoOgZpkhVVSVIHHzd4F6NiCLQ{WwQVAvQCEQvF2= NYHoc5dqOjZ6NEGxOlg>
MCF7 cells NX31enBFWHKxbHnm[ZJifGmxbjDhd5NigQ>? Mm\WPVYhcA>? MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdHWuYYKgSG5CKGOxboTlcpQh[W[2ZYKgPVYhcHK|IHL5JGN6WVWDTmSgUmYh\my3b4Lld4NmdmOnIHHzd4F6NiCLQ{WwQVAvQSEQvF2= MoO0NlY4OzF|MEC=
HepG2 cells NWnRTHpkS3m2b4TvfIlkcXS7IHHzd4F6 NGi2PHUzPCCq NHjpPXREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiMkSgbJJ{KGK7IF3UV{Bie3OjeT6gTWM2OD1yLkmg{txO Moi4NlY5PDFzNki=
MDA-MB-435 cells MkTlR5l1d3SxeHnjbZR6KGG|c3H5 M4LGPVczKGh? MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XlwIFnDOVA:OS5zNDFOwG0> MYCyNFM4OTF6Mx?=
LS174T cells MUXDfZRwfG:6aXPpeJkh[XO|YYm= MoTLPVYhcA>? Ml;jR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUHMyPzSWIHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZkvKEmFNUC9NU4yPiEQvF2= MnP4NVg2OTN7N{[=
T84 cells M2LiUXBzd2yrZnXyZZRqd25iYYPzZZk> NIC5fYY6PiCq M4HYTmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVEi0JINmdGy|IHHzd4V{e2WmIHHzJINmdGy3bHHyJGRPSSClb370[Y51KGGodHXyJFk3KGi{czDifUBEgVGXQV7UJG5HKG[udX;y[ZNk\W6lZTDhd5NigS5iSVO1NF0yNjJizszN MUmyOlc{OTNyMB?=
SW480 cells NUnpW4hUS3m2b4TvfIlkcXS7IHHzd4F6 NE\TOnQzPCCq MnLuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV3c1QDBiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XlwIFnDOVA:OS52IN88US=> MkKwNlY5PDFzNki=
K562 cells NWDRcVJsWHKxbHnm[ZJifGmxbjDhd5NigQ>? NUnGWoJ5PzJiaB?= NUnJVWd{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIFnfGW{IEeyJIhzey5iSVO1NF0yNjlizszN M2GySVI2PDJyMUe1
HT-29 cells NFTDSo1Rem:uaX\ldoF1cW:wIHHzd4F6 MX[3NkBp MkfuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVD2yPUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyudXzhdkBFVkFiY3;ueIVvfCCjZoTldkA4OiCqcoOgZpkhS3mTVVHOWEBPTiCobIXvdoV{[2WwY3WgZZN{[XlwIFnDOVA:OS57IN88US=> M4D0VFI3PzNzM{Cw
HCT116 cells M1\pR3Bzd2yrZnXyZZRqd25iYYPzZZk> M{e4NVI1NTd{IHi= MlzjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiMkSgeI8hPzJiaILzJIJ6KFOUQjDhd5NigS5iSVO1NF0zKM7:TR?= MlXZNlY2QTV6N{W=
Hep3B cells NUeyPZo6WHKxbHnm[ZJifGmxbjDhd5NigQ>? NXXWOYVqPzJiaB?= MmXDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYCzRkBk\WyuczDh[pRmeiB5MjDodpMh[nliWGTUJIF{e2G7LjDJR|UxRTRwN{Og{txO M1[xOlE6Pzl4OUW2
Hep3B cells MnLyS5Jwf3SqIHnubIljcXSxbjDhd5NigQ>? MWK3NkBp MoHVS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTIVxO0JiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHhVXCCjc4PhfU4hUUN3ME20Mlc{KM7:TR?= NUf3b5IyOjJyN{myOVQ>
LoVo cells MX;DfZRwfG:6aXPpeJkh[XO|YYm= M2jZTlczKGh? NWj4RmlXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVG:YbzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NiCLQ{WwQVQvQTlizszN NVXsTGduOjB|N{GxPFM>
KBH5.0 cells NYPyWXA1S3m2b4TvfIlkcXS7IHHzd4F6 M2jWdGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDUDVwMDDj[YxteyCkeTDNWHQhdWW2aH;kMkBKSzVyPUeuOEDPxE1? NFfaeXQyQDd5MUmzNC=>
H3347 cells Ml\RR5l1d3SxeHnjbZR6KGG|c3H5 MUK3NkBp NXXPOJcxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDN|NEegZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IFHsZY1ieiCEbIXlJIF{e2G7LjDJR|UxRTdwNUOg{txO MYiyOFU5OzN3NR?=
HCT15 cells MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 MmHiNlQuPzJiaB?= M{Py[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVUh[2WubIOgZYZ1\XJiMkSgeI8hPzJiaILzJIJ6KFOUQjDhd5NigS5iSVO1NF05NjVizszN NUn1e4VDOjZ3OUW4O|U>

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western blot
AMPK / p-AMPK / mTOR / p-mTOR / p70S6K / p-p70S6K; 

PubMed: 25973791     


Western blot showing the protein levels of AMPK, p-AMPK, mTOR, p-mTOR, p70S6K, and p-p70S6K in LoVo and LoVo-R8 with or without irinotecan. 

NFκB p65 / phospho-NFκB p65 / NFκB p50 / IκBα / p27Kip1; 

PubMed: 22206574     


For Western blot analysis (B), cytoplasmic proteins were analyzed using antibodies against NFκB p65 and p50, phospho-NFκB p65, IκBα and p27Kip1. In the presence of irinotecan, there was a loss of cytoplasmic NF-κBp65, but in the presence of sorafenib, this loss was greatly reduced, corresponding to a decrease in phosphorylation of NF-κBp65. In addition, compared to treatment with sorafenib or irinotecan alone, there was increased expression of IκBα following treatment with sorafenib and irinotecan. Lastly, following treatment with irinotecan and sorafenib irinotecan combination, there was decreased expression of p27Kip1 compared to sorafenib treatment alone.

TopI / pAKT / pMEK / pERK / p-p38 MAPK / pJNK2; 

PubMed: 29237470     


a Gimatecan significantly inhibited the expression of TopI, pAKT, pMEK, and pERK, and activated the expression of p-p38 MAPK and pJNK2 in SNU-1 cells. b Gimatecan significantly inhibited the expression of pAKT and pERK in NCI-N87 cells. Cells were starved in serum-free medium overnight, exposed to gimatecan or irinotecan for 48 h and harvested at 70–80% confluence. Total protein of SNU-1 and NCI-N87 was extracted and the expression of TopI, pAKT, pMEK, pERK, p-p38 MAPK and pJNK2 were assessed by western-blotting 

25973791 22206574 29237470
Immunofluorescence
NFκB; 

PubMed: 22206574     


BT12 cells were incubated with sorafenib or vehicle for 30 minutes followed by treatment with irinotecan for an additional 2 hours. For indirect immunofluorescence (A), cells were fixed and stained with antibodies to NF-κB followed by fluorescent labeled secondary antibodies. Concurrent DAPI stain was used to localize the nuclei (lower panel). Slides were visualized using a fluorescent microscope and random fields were photographed. The cytoplasmic staining seen in untreated and sorafenib treated cells was significantly reduced following treatment with irinotecan. However, the addition of sorafenib enabled the cells to maintain cytoplasmic staining in the presence of irinotecan. 

22206574
Growth inhibition assay
Cell viability; 

PubMed: 25973791     


The sensitivity of eight colon cancer cell lines to irinotecan was measured using the CCK-8 assay. For the CCK-8 assay, cells were exposed to irinotecan at given concentrations for 72 h before measurement. The cell viability was presented as the percentage relative to untreated cells.

25973791
体内試験 In COLO 320 xenografts, Irinotecan induces a maximum growth inhibition of 92%. [5] A single dose of Irinotecan significantly increases amounts of topoisomerase I covalently bound to DNA in stomach, duodenum, colon and liver. Concomitantly, the Irinotecan-treated group shows significantly higher amounts of DNA strand breaks in colon mucosa cells compared to the control group. [6]

お薦めの試験操作(参考用のみ)

細胞試験:

[1]

- 合併
  • 細胞株: LoVo and HT-29 cells
  • 濃度: 0 μM -100 μM
  • 反応時間: 48 hours
  • 実験の流れ:

    Exponentially growing cells (LoVo and HT-29 cells) are seeded in 20 cm2 Petri dishes with an optimal cell number for each cell line (2 × 104 for LoVo cells, 105 for HT-29 cells). They are treated 2 days later with increasing concentrations of Irinotecan or SN-38 for one cell doubling time (24 hours for LoVo cells, 40 hours for HT-29 cells). After washing with 0.15 M NaCl, the cells are further grown for two doubling times in normal medium, detached from the support with trypsin-EDTA and counted in a hemocytometer. The IC50 values are then estimated as the Irinotecan or SN-38 concentrations responsible for 50% growth inhibition as compared with cells incubated without Irinotecan or SN-38.


    (参考用のみ)
動物試験:

[5]

- 合併
  • 動物モデル: Female nude mice with COLO 320 and WiDr xenografts.
  • 投薬量: 20 mg/kg
  • 投与方法: Intraperitoneal injection
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 100 mg/mL (147.67 mM)
Water 1 mg/mL (1.47 mM)
Ethanol '7 mg/mL '7
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5% DMSO+saline
混合させたのち直ちに使用することを推奨します。
20mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 677.18
化学式

C33H38N4O6.HCl.3H2O

CAS No. 136572-09-3
Storage powder
in solvent
別名 CPT-11 HCl Trihydrate
Smiles O.O.O.Cl.CCC1=C2CN3C(=O)C4=C(C=C3C2=NC5=CC=C(OC(=O)N6CCC(CC6)N7CCCCC7)C=C15)C(O)(CC)C(=O)OC4

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04238819 Not yet recruiting Drug: Abemaciclib|Drug: Irinotecan|Drug: Temozolomide Relapsed Solid Tumor|Refractory Solid Tumor Eli Lilly and Company July 31 2020 Phase 1
NCT04158349 Not yet recruiting Drug: Oxaliplatin|Drug: mFOLFIRI Colorectal Cancer|Appendiceal Cancer|Peritoneal Carcinoma University of Utah May 2020 Phase 1
NCT04164979 Recruiting Drug: Cabozantinib|Drug: Pembrolizumab Gastric Adenocarcinoma|GastroEsophageal Cancer University of California Irvine|Exelixis February 4 2020 Phase 2

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

Topoisomeraseシグナル伝達経路

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID